Fluorescent Enzymatic Sensor Based Glucose Oxidase Modified Bovine Serum Albumin‐Gold Nanoclusters for Detection of Glucose

Abstract

An enzymatic fluorescent probe is developed for the selective detection of glucose. In this work, a Bovine Serum Albumin stabilized gold nanocluster (BSA‐AuNCs) was synthesized by microwave assisted method, and it is modified with glucose oxidase, thereby a fluorescent enzymatic sensor (BSA‐AuNCs@GoX) was designed for the sensitive detection of glucose with a limit of detection of 0.03 mM. The red fluorescence exhibited by the probe is quenched by the production of H2O2 on addition of glucose via. a static quenching mechanism from UV visible absorption and Fluorescence lifetime results. The developed probe exhibits good selectivity and sensitivity with other coexisting molecular species such as glycine, creatinine, methionine, histidine, uric acid, albumin, and ions such as sodium, potassium, calcium, magnesium, zinc etc. that appear in the body fluid. The practical applicability was studied in paper strip and extended its reproducibility in biological matrixes such as human serum and urine and found a good recovery percentage of 94‐101%. By this way, we have fabricated an effective fluorescent enzymatic “turn‐off” sensing probe for the detection of glucose.

Mo(IV) Ion-Modulated BSA-Protected Gold Nanocluster Probe for Fluorescence Turn-On Detection of Trimethylamine N-Oxide (TMAO)

“…In acidic media, the stability of the AuNCs becomes unstable, because electrons are transferred from the conduction band of BSA@AuNCs to the medium. On the other hand, in an alkaline medium, the luminescence intensity is strong because the OH ions in the protein-protected AuNCs are stabilized. , The fluorescence of BSA@AuNCs was reduced when the concentration of Mo 4+ increased within the range of 0–11.5 mg, as shown in Figure A. To ensure reversibility in the luminescence switching response toward TMAO, the gold nanocluster was accurately quenched with a concentration of Mo 4+ that was ideally restricted to 11.5 mg.…”