Fluorescent‐functionalized graphene oxide for selective labeling of tumor cells

Abstract

Fluorescence probe has attracted significant attention for biomedical imaging in recent years due to their high resolution at the cellular level. Organic‐based fluorescent probes with high quantum yield are widely applied in bioimaging, but most of them suffer from a serious obstacle called aggregation‐caused quenching in cellular systems. New fluorophore has been designed through functionalization of graphene oxide which emphatically exhibits aggregation‐induced emission along with pH‐responsive nanoprobe. Significantly higher emission of this material in slightly acidic media helps to detect tumor cell by creating a sharp contrast with the image of normal cells. The reason for pH‐induced enhanced emission phenomenon is revealed through aggregation of sulfonated species in acidic media. Furthermore, the biocompatible nature of the newly developed material is found to be suitable for its application in biomedical imaging for cancer detection with better accuracy at lower cost. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1917–1924, 2019.

Wireless Network of Silver Film Lysozyme Aptasensor Based on Fractal Measurement

“…rombin, cytochrome C, and lysozyme were all purchased from Sigma-Aldrich Co. (USA). GO was synthesized in the laboratory using Hummer's method [34,35]. Polyethylene glycol methacrylate (PEGMA) and 2hydroxyethyl methacrylate (HEMA), monomers for the polymer buffer layer fabrication, were purchased from Sigma-Aldrich.…”

Layered Double Hydroxide Nanoparticles for Efficient Gene Delivery for Cancer Treatment

“…The cell viability assay of LDH samples were measured with MTT assay using both HeLa and NIH-3T3 cells . In brief, 1 × 10 5 cm –2 of both types of cells (HeLa and NIH-3T3) cells were seeded onto the 96 culture plates in DMEM medium.…”

“…The cell viability assay of LDH samples were measured with MTT assay using both HeLa and NIH-3T3 cells. 37 In brief, 1 × 10 5 cm −2 of both types of cells (HeLa and NIH-3T3) cells were seeded onto the 96 culture plates in DMEM medium. After 1 day of culture, the growth media in the wells was replaced with the fresh DMEM containing the samples in the concentrations range of 10−100 μg/mL and were incubated for 1, 2, and 3 days.…”

Isomerization-Induced Excimer Formation of Pyrene-Based Acylhydrazone Controlled by Light- and Solvent-Sensing Aromatic Analytes

“…Fluorescent sensors are attractive and useful tools for chemical sensing due to their low cost, high sensitivity, rapid response, and technical simplicity. − In recent decades, a large number of excellent fluorescent sensor systems have been developed by introducing fluorophore moieties into the main building block. , A good fluorophore should have good luminescent and satisfactory photophysical properties. To date, scientists have synthesized and utilized some known chromophores or their derivatives such as fluorescein, borro-dipyrromethene (BODIPY), tetraphenylethylene, rhodamine, and fluorescent polymers like polythiophene for sensing applications. ,,, As a fluorogenic unit, pyrene is one of the most useful fluorescence probes due to its expanded π electron delocalization, excellent UV–vis absorption and fluorescence properties, high quantum yield, and greater ability to form excimers.…”

“…19−23 In recent decades, a large number of excellent fluorescent sensor systems have been developed by introducing fluorophore moieties into the main building block. 24,25 A good fluorophore should have good luminescent and satisfactory photophysical properties. To date, scientists have synthesized and utilized some known chromophores or their derivatives such as fluorescein, borrodipyrromethene (BODIPY), tetraphenylethylene, rhodamine, and fluorescent polymers like polythiophene for sensing applications.…”