Fluorescent‐guided surgery for sentinel lymph node detection in gastric cancer and carcinoembryonic antigen targeted fluorescent‐guided surgery in colorectal and pancreatic cancer

Vuijk, Floris A.; Hilling, Denise E.; Mieog, J. Sven D.; Vahrmeijer, Alexander L.

doi:10.1002/jso.25139

Cited by 35 publications

(18 citation statements)

References 85 publications

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“…Особенно эти преимущества вы ражены при лапароскопических операциях, которые все чаще и чаще выполняются в мире при указанных локализациях. Первым основным преимуществом флюоресцентной лимфографии является визуализация лимфоузлов, что, несомненно, улучшает качество лимфодиссекции, как показал ряд работ [1,2].…”

Section: результаты исследований и их обсуждениеunclassified

“…Поэтому требуются более масштабные исследования. В перспективе возможно появление ещё одного преимущества: технология моноклональных антител, связанных флюоресцирующим препаратом [1]. Это откроет перспективу к воплощению концепции сторожевого лимфоузла на качественно новом уровне.…”

Section: результаты исследований и их обсуждениеunclassified

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Первый Опыт Применения Флюоресцентной Лимфографии При Раке Желудка

Malinovsky¹,

Galimon²,

Badion³

2019

ШХ

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Цель работы: оценка результатов первого опыта применения флюоресцентной лимфографии в ходе лимфодиссекции D2 при раке желудка.Материалы и методы. Флюоресцентная лимфография выполнена двум пациентам. Первому пациенту с раком верхней трети желудка без проростания кардии с регионарной лимфаденопатией была выполнена гастрэктомия с лимфодиссекцией D2.Послеоперационных осложнений не было. Начиная с 5-х суток проводилось зондовое питание. На 10 сутки зонд извлечёни начато энтеральное питание. Больной выписан на 12 сутки. Второму пациенту с инфильтративной опухолью антрального отдела со стенозом привратника и регионарной лимфаденопатией выполнена субтотальная резекция с лимфодиссекцией D2. В послеоперационном периоде развился наружный панкреатический свищ, закрывшийся через 14 суток. Зонд извлечен на 10 сутки. Больной выписался на 15 сутки. В обоих случаях в начале операции перитуморозно введено по 10 мл растворённого индоцианина зелёного. Использовалась специальная видеосистема фирмы KarlStorz с оптикой-экзоскопом Vitom II.Результаты исследований и их обсуждение. Через 50–60 минут в обоих случаях при осмотре в свете, близком к инфракрасному, была зафиксирована не только флюоресценция первичной опухоли, но и достаточно яркое свечение регионарных лимфоузлов, причём более активная флюоресценция наблюдалась в лимфоузлах, относящихся к основному пути лимфоотока. Также флюоресценция позволила визуализировать лимфоузлы в толще жировой ткани, которые на первый взгляд не определялись, и позволила проконтролировать их полное отсутствие по завершению систематической лимфодиссекции. После завершения лимфодиссекции флюорисцирующих лимфоузлов не наблюдалось. Таким образом, флюоресцентная лимфография позволила проконтролировать полноту выполненной лимфодиссекции.

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Section: результаты исследований и их обсуждениеunclassified

Первый Опыт Применения Флюоресцентной Лимфографии При Раке Желудка

Malinovsky¹,

Galimon²,

Badion³

2019

ШХ

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“…The emergence of "molecular imaging" have led to a boom in studies regarding tumor diagnosis (6,7), the specificity of antibodies for antigens over expressed or uniquely expressed in tumor cells makes them ideal candidates in the development of bioconjugates for tumor imaging, the molecular probes are being used to detect tumors or neoplastic lesions through fluorescent endoscopy and are being widely applied in surgical navigation (8). The studies had investigated that the carcinoembryonic antigen (CEA) targeted fluorescent imaging was successfully used in both pancreatic and colorectal cancer (9), In 2018, the carcinoembryonic antigen related cell adhesion molecule5 (SGM-101) fluorescent probe was used in the diagnosis of pancreatic cancer and the metastases, with an accuracy of 84% and sensitivity of 98% (10). Nevertheless, similar investigations in EGC diagnosis remain insufficient.…”

Section: Introductionmentioning

confidence: 99%

Carcinoembryonic Antigen Related Cell Adhesion Molecule 6 Promotes Carcinogenesis of Gastric Cancer and Anti-CEACAM6 Fluorescent Probe Can Diagnose the Precancerous Lesions

Zheng

Zhang

et al. 2021

Front. Oncol.

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The diagnosis of precancerous lesions or early gastric cancer (EGC) is very important for patient survival. Molecular imaging is a visualized method that can easily and precisely diagnose tumors. However, there are currently few studies about molecular imaging diagnosis of EGC. Here, we studied the expression of carcinoembryonic antigen related cell adhesion molecule 6 (CEACAM6) in the progression of GC. Then, the regulatory roles of CEACAM6 in GC cells were investigated. Furthermore, both the fluorescent-labeled and near infrared molecular-labeled probes were synthesized, and the diagnostic value of anti-CEACAM6 probes in GC was evaluated in vivo using a GC mice model as well as in vitro using fresh dysplastic gastric mucosa obtained from endoscopic submucosal dissection (ESD) operations. Our study showed that CEACAM6 was over expressed in GC tissues compared to adjacent tissues, and the patients with higher CEACAM6 expression had lower survival time. Moreover, the CEACAM6 expression was higher in the dysplastic gastric mucosa than in the adjacent normal mucosa. CEACAM6 accelerated the growth, proliferation, and invasion of GC cells in the in vitro and in vivo studies. Moreover, up regulated CEACAM6 can induce the expression of proteins related to GC progression. Furthermore, the anti-CEACAM6 probes exhibited good affinity with GC cell lines. The probes can track tumors as well as metastases in the mice model in vivo, and can precisely identify the area of dysplastic gastric mucosa using specimens obtained from ESD operations by wide field fluorescent endoscopy. The surface micro features of the mucosa can also be observed using fluorescent micro endoscopy, and the degree of atypia can be distinguished by both the signal intensity and surface micro morphology. CEACAM6 is a key molecular marker in GC progression, and the anti-CEACAM6 probe-assisted fluorescent endoscopy may be a potential option for the diagnosis of precancerous lesions.

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“…The NIRF is able to provide high-contrast images since its scattering and tissue auto-fluorescence is relatively low [9]. Therefore, NIRF has been utilized for different targets such as tumors [10][11][12], sentinel lymph nodes [13][14][15][16], cardiovascular diseases [17,18], and neurological diseases [19,20]. As small molecules, near-infrared (NIR) fluorescent molecules have been used as contrast agents in surgery since they can provide surgical guidance based on the EPR effect of tumor tissues [21].…”

Section: Introductionmentioning

confidence: 99%

Tumor-Targeting Glycol Chitosan Nanoparticles for Image-Guided Surgery of Rabbit Orthotopic VX2 Lung Cancer

Rho

Yoon

et al. 2020

Pharmaceutics

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Theranostic nanoparticles can deliver therapeutic agents as well as diverse imaging agents to tumors. The enhanced permeation and retention (EPR) effect is regarded as a crucial mechanism for the tumor-targeted delivery of nanoparticles. Although a large number of studies of the EPR effect of theranostic nanoparticles have been performed, the effect of the change in the body size of the host on the EPR effect is not fully understood. In this regard, comparative research is needed on the behavior of nanoparticles in large animals for developing the nanoparticles to the clinical stage. In this study, we prepared fluorophore (indocyanine green (ICG) or cyanine 5.5 (Cy5.5))–conjugated glycol chitosan nanoparticles (CNPs) for comparing the tumor-targeting efficacy in VX2 tumor-bearing mouse and rabbit models. As expected, the CNPs formed nano-sized spherical nanoparticles and were stable for 8 days under aqueous conditions. The CNPs also exhibited dose-dependent cellular uptake into VX2 tumor cells without cytotoxicity. The half-life of the near-infrared fluorescence (NIRF) signals in the blood were 3.25 h and 4.73 h when the CNPs were injected into mice and rabbits, respectively. Importantly, the CNPs showed excellent tumor accumulation and prolonged biodistribution profiles in both the VX2 tumor-bearing mouse and rabbit models, wherein the tumor accumulation was maximized at 48 h and 72 h, respectively. Based on the excellent tumor accumulation of the CNPs, finally, the CNPs were used in the image-guided surgery of the rabbit orthotopic VX2 lung tumor model. The lung tumor tissue was successfully removed based on the NIRF signal from the CNPs in the tumor tissue. This study shows that CNPs can be potentially used for tumor theragnosis in small animals and large animals.

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Fluorescent‐guided surgery for sentinel lymph node detection in gastric cancer and carcinoembryonic antigen targeted fluorescent‐guided surgery in colorectal and pancreatic cancer

Cited by 35 publications

References 85 publications

Первый Опыт Применения Флюоресцентной Лимфографии При Раке Желудка

Первый Опыт Применения Флюоресцентной Лимфографии При Раке Желудка

Carcinoembryonic Antigen Related Cell Adhesion Molecule 6 Promotes Carcinogenesis of Gastric Cancer and Anti-CEACAM6 Fluorescent Probe Can Diagnose the Precancerous Lesions

Tumor-Targeting Glycol Chitosan Nanoparticles for Image-Guided Surgery of Rabbit Orthotopic VX2 Lung Cancer

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