Fluorescent Probe ABM for Screening Gastrointestinal Patient’s Immune State

Abstract

The fluorescent probe-aminoderivative of benzanthrone, ABM (developed at Riga Technical University, Riga, Latvia) was used to characterize the membranes of lymphocytes of cancer patients: 46 patients with gastrointestinal diseases, 13 patients having different primary localizations with massive metastases and intoxication. Patients were divided into three groups: (1) with decreased fluorescence intensity, (2) normal fluorescence intensity, (3) increased fluorescence intensity. The lymphocytes distribution among subsets differed between groups, in correspondence to the level of fluorescence intensity. Surgical treatment affected the main immunological parameters and elevated the functional activity of lymphocytes. In the advanced tumors group, fluorescence intensity correlates with the survival rate. Results suggest that determination of lymphocytes functional activity by ABM can aid evaluation of the immune status in cancer patients.

Altered plasma albumin characteristics and lymphocyte populations in gastrointestinal cancer patients: Detection using modified fluorescence responses

“…The choice to examine albumin, among the myriad of constituents in plasma, is that this protein is practically the single source of ABM binding and subsequent fluorescence in plasma (Grizunov and Dobretsov, 1994). Our earlier studies showed that within plasma, albumin is nearly alone in binding with ABM with a very high level of selectivity (Kalnina et al, 1996(Kalnina et al, , 2004(Kalnina et al, , 2007a. The distribution of ABM fluorescence (intensity) within fractions of human plasma was seen to be albumin >>> globulins >> non-specific binding by other components (i.e., 90%, ≈ 5%, < 1%, respectively).…”

“…Investigations in this study, as in earlier studies, were performed using the fluorescent probe ABM (Kalnina and Meirovics, 1999;Kalnina et al, 2006Kalnina et al, , 2007a. This 3-aminobenzanthrone probe ( Figure 1) was synthesized by substituting a bromine atom into 3-bromobenzanthrone using an appropriate amine.…”

“…The chemical structure of the ABM bears a resemblance to the fluorescent probe MBA (3-methoxybenzanthrone), but having a substituted amino group at the 3-position of the benzanthrone molecule. While the use of MBA is limited by two properties [(1) it destroys cells after a short period of time and (2) it fades rapidly in fluorescent light (≈ 80% loss in 6-8 min)], our previous studies have shown that ABM is both photostable and non-toxic to/in cells (Kalnina et al, 2007a).…”

Fluorescent Biomarker in Colorectal Cancer

“…Fluorescence intensity of ABM in lymphocytes suspension tended to decrease with progression of cancer. Shifts in magnitude of ABM fluorescence could reflect modifications in one/more interdependent properties of cells (Kalnina et al, 2007). As seen in the studies mentioned here, at least two parameters are responsible for this phenomenon.…”

“…The choice to examine albumin, among the myriad of constituents in plasma, is that this protein is practically the single source of ABM binding and subsequent fluorescence in plasma Dobretsov, 1994, 1998). Our earlier studies showed that within plasma, albumin is nearly alone in binding with ABM with a very high level of selectivity (Kalnina et al, 1996(Kalnina et al, , 2004(Kalnina et al, , 2007. The distribution of ABM fluorescence (intensity) within fractions of human plasma was seen to be albumin >>> globulins >> non-specific binding by other components (i.e., 90%, ≈ 5%, < 1%, respectively).…”