Fluorescent silver nanoparticle based highly sensitive immunoassay for early detection of HIV infection

Abstract

For the first time, we have engineered streptavidin labeled fluorescent silver nanoparticles for their application in immunosensing of biomolecules which will significantly increase sensitivity without compromising the specificity. Plasma samples that were HIV Àve showed no interference with detection of HIV-1 p24 antigen. This technology can be used in resource limited settings and easily adopted for the detection of other pathogen antigens.

Streptavidin-conjugated gold nanoclusters as ultrasensitive fluorescent sensors for early diagnosis of HIV infection

“…The immunocomplex then attaches to the AuNC-SA, which recognizes and interacts with the above biotinylated sandwich complex. After multiple wash steps, the signal released from the wells is recorded with a SpectraMax M5 multiplate reader ( 27 ).…”

Femtogram Level Sensitivity achieved by Surface Engineered Silica Nanoparticles in the Early Detection of HIV Infection

“… S. No. Method P24 Concentration detected in pg/mL Reference 1 Conventional Elisa 10–15 16 2 Inductively coupled plasma mass spectrometry based gold nanoparticle immunoassay 7.5 35 3 Europium nanoparticle based immunoassay 0.5 15 4 Capacitive Immunosensor 0.12 36 5 Microchip based Europium nanoparticle immunoassay 5 37 6 Single-molecule immunosorbent assay 0.1 38 7 ZnO nanoparticle based immunoassay 25 39 8 Fluorescent Silver nanoparticles immunoassay 10 40 9 P24 antigen detection assay with booster step 0.5 17 10 Immunofluorescent cytometric bead assay 0.4 41 11 Carbon Dots based immunoassay 20 42 12 Silica Nanoparticle based immunoassay 0.02 Current Study …”

Strategies for the Development of Metallic‐Nanoparticle‐Based Label‐Free Biosensors and Their Biomedical Applications

“…Kurdekar and co‐workers observed the early‐stage detection of HIV p24 antigen infection by using fluorescent AgNPs as an immunoassay (FSNIA) . Immunoassay is a method of binding an antigen to an antibody for the recognition of a pathogen .…”

“…[71,72] Kurdekar and co-workerso bserved the early-stage detection of HIV p24 antigen infection by using fluorescent AgNPsa sa n immunoassay (FSNIA). [73] Immunoassay is am ethod of binding an antigen to an antibodyf or the recognition of ap athogen. [74][75][76] As showni nF igure 3, captured antibodies were immobilized on microplates following incubation and washing with blocking buffer to preventthe protein from reaching nonspecific sites.…”

“…Later,b iotinylated antibody caps the p24 antigen following the interaction of fluorescent AgNP (FSN), which fluoresces after exposuretot he excitationsource. Adapted from ref [73]. with permission.…”