Fluoride: A review of pre-clinical and clinical studies

Sharma, Divya; Singh, Aarti; Verma, Kanika; Paliwal, Sarvesh; Sharma, Swapnil; Dwivedi, Jaya

doi:10.1016/j.etap.2017.10.008

Cited by 79 publications

(40 citation statements)

References 99 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In the current study, NaF produced dilatation and congestion of the pancreatic blood vessels and extravasations of blood cells into the interstitial spaces between the pancreatic lobules. These findings are similar to the results of Sharma et al [11] and Guney et al [29] who found congestion of blood vessels and infiltration of the blood cells in the surrounding area.…”

Section: Discussionsupporting

confidence: 92%

“…The ultrastructural examinations of the NaFtreated animals' pancreas of the current study revealed mitochondrial vacuolation and balloon-shaped ones with ill-defined cristae. These findings are similar to the results of Sharma et al [11] who reported the most significant morphological changes observed in the tissues exposed to NaF-treated animals included damage to mitochondria. Also, the ultrastructural examinations of the NaF-treated animals' pancreas of the current study revealed that the zymogen granules were scanty.…”

Section: Discussionsupporting

confidence: 92%

“…Daily ingestion of about 1-2 ppm of fluoride found in artificially fluoridated water can result in melena, hematemesis, faintness, shallow breathing, stomach cramps, tremors, unusual increase in saliva, watery eyes, weakness, constipation, loss of appetite, skin rash, mouth and lips sores, and white, brown or black teeth discoloration [11,12] .…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Possible Protective Role of Methionine against Sodium Fluoride-Induced Pancreatic Changes in the Adult Male Albino Rat: A Histological, Immunohistochemical and Morphometric Study

Zaghloul

Gad-Elrab

Bushra

et al. 2019

Egyptian Journal of Histology

View full text Add to dashboard Cite

Background: Excess fluorides intake produces histopathological changes of many organs. Methionine is a potential natural antioxidant against oxidative radicals. Aim of the Work: To evaluate the possible protective role of methionine against sodium fluoride (NaF)-induced pancreatic toxicity. Material and Methods: Thirty 3-months (200-250gm) adult male albino rats were divided into three equal groups: group I (control), group II (Fluoride group) and group III (Fluoride+methionine group). Control group; was given 1ml distilled water. Fluoride group; was given 10 mg NaF/kg b.w. Fluoride+methionine group; was given 10 mg NaF/kg b.w. and 2 mg methionine/rat. All the treatment was given orally by gastric tube once daily for 35 days. After anesthesia, all groups were sacrificed. The pancreatic specimens were prepared for light and electron microscopic studies and anti-insulin antibody immunohistochemical staining. The mean numbers of zymogen granules and insulin positive β-cells of all groups were counted. Results: The mean numbers of zymogen granules and insulin positive β-cells of the fluoride group were significantly decreased when compared to control. The pancreatic specimens of the fluoride group revealed congested blood vessels, extravasated blood cells, vacuolated pancreatic acini, loss of the acinar cell architecture, dilated rough endoplasmic reticulum and degenerated mitochondria. By anti-insulin antibodies immunohistochemistry, there was a weak positive reactivity in the fluoride treated group when compared to control. The concomitant administration of NaF and methionine improved these changes. Conclusion: The concurrent administration of NaF and methionine ameliorates the structural alterations developed in the pancreas following excess NaF intake.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Possible Protective Role of Methionine against Sodium Fluoride-Induced Pancreatic Changes in the Adult Male Albino Rat: A Histological, Immunohistochemical and Morphometric Study

Zaghloul

Gad-Elrab

Bushra

et al. 2019

Egyptian Journal of Histology

View full text Add to dashboard Cite

show abstract

“…However, another alternative for caries and periodontal disease treatment is based on bacterial activity inhibition by means of medicinal plants. They provide low cost solutions for the community, are less invasive and don't have toxicity associated problems, as is the case of fluoride [25,26]. Various studies with plant extracts and secondary metabolites report high antimicrobial activity.…”

Section: Introductionmentioning

confidence: 99%

Preliminary Phytochemical Analysis of Berberis goudotii Triana & Planch. ex Wedd. (Berberidaceae) with Anticariogenic and Antiperiodontal Activities

et al. 2019

View full text Add to dashboard Cite

Berberis goudotii is an endemic Colombian plant found in the paramo ecosystem. It has been used in food preparation and as a medicinal plant for diverse treatments. Additionally, it is used as a mouthwash to strengthen the gums and combat throat irritations and periodontitis. The present research evaluated Berberis goudotii aerial parts extract and fractions antimicrobial activities. Ultrasonic-assisted extraction was used to attain total ethanol-water extract. Solid-liquid fractionation was used to obtain hexane fraction. The residue was dispersed in water and liquid-liquid fractionation was carried-out to acquire dichloromethane, butanol and water fractions. Preliminary phytochemical analysis was performed on total extract and phenol, polyphenol, flavonoid, and proanthocyanidin, while tannin content was quantified. Antimicrobial activity assessment was performed by agar diffusion method using disks and wells employing Ceftazidime as a positive control against Streptococcus mutans, Streptococcus sobrinus, Lactobacillus acidophilus, Lactobacillus casei, Porphyromonas gingivalis, Prevotella intermedia and Fusobacterium nucleatum. Antimicrobial activity was determined as relative percentage inhibition (RPI), minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Phenols (92.5 ± 7.7 mg GA/10 g), polyphenols (87.7 ± 8.1 mg PG/10 g) and tannins (44.1 ± 4.3 mg PG/10 g) were among the highest secondary metabolites observed. Total extract presented an MBC of 1.0 µg/µL against cariogenic bacteria (Streptococcus mutans and Streptococcus sobrinus) and 0.12 µg/µL against bacteria associated with periodontal disease (Porphyromonas gingivalis, Prevotella intermedia and Fusobacterium nucleatum). Butanol and hexane fractions showed antiperiodontal activity with MBC of 0.12 and 1.0 µg/µL, respectively. In conclusion, Berberis goudotii total extract demonstrated antimicrobial activity against cariogenic and periodontal microorganisms, on the other hand, hexane and butanol fractions displayed antiperiodontal activity.

show abstract

“…Fluoride can cross both the blood-brain barrier and the plasma membrane of cells [16], allowing this ion to accumulate in the brain [17], where it can damage neurons [18]. Fluoride injures the central nervous system (CNS) by several mechanisms [18][19][20], in particular by elevating the level of oxidative stress [21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Exposure to fluoride aggravates the impairment in learning and memory and neuropathological lesions in mice carrying the APP/PS1 double-transgenic mutation

et al. 2019

View full text Add to dashboard Cite

Background: Alzheimer's disease (AD) is responsible for 60-70% of all cases of dementia. On the other hand, the tap water consumed by hundreds of millions of people has been fluoridated to prevent tooth decay. However, little is known about the influence of fluoride on the expression of APP and subsequent changes in learning and memory and neuropathological injury. Our aim here was to determine whether exposure to fluoride aggravates the neuropathological lesions in mice carrying the amyloid precursor protein (APP)/presenilin1 (PS1) double mutation. Methods: These transgenic or wide-type (WT) mice received 0.3 ml of a solution of fluoride (0.1 or 1 mg/ml, prepared with NaF) by intragastric administration once each day for 12 weeks. The learning and memory of these animals were assessed with the Morris water maze test. Senile plaques, ionized calcium binding adaptor molecule 1 (Iba-1), and complement component 3 (C3) expression were semi-quantified by immunohistochemical staining; the level of Aβ42 was detected by Aβ42 enzyme-linked immunosorbent assays (ELISAs); the levels of synaptic proteins and enzymes that cleave APP determined by Western blotting; and the malondialdehyde (MDA) content and activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) measured by biochemical procedures. Results: The untreated APP mice exhibited a decline in learning and memory after 12 weeks of fluoride treatment, whereas treatment of these some animals with low or high levels of fluoride led to such declines after only 4 or 8 weeks, respectively. Exposure of APP mice to fluoride elevated the number of senile plaques and level of Aβ42, Iba-1, and BACE1, while reducing the level of ADAM10 in their brains. The lower levels of synaptic proteins and enhanced oxidative stress detected in the hippocampus of APP mice were aggravated to fluoride. Conclusions: These findings indicate that exposure to fluoride, even at lower concentration, can aggravate the deficit in learning and memory and neuropathological lesions of the mice that express the high level of APP.

show abstract

Fluoride: A review of pre-clinical and clinical studies

Cited by 79 publications

References 99 publications

The Possible Protective Role of Methionine against Sodium Fluoride-Induced Pancreatic Changes in the Adult Male Albino Rat: A Histological, Immunohistochemical and Morphometric Study

The Possible Protective Role of Methionine against Sodium Fluoride-Induced Pancreatic Changes in the Adult Male Albino Rat: A Histological, Immunohistochemical and Morphometric Study

Preliminary Phytochemical Analysis of Berberis goudotii Triana & Planch. ex Wedd. (Berberidaceae) with Anticariogenic and Antiperiodontal Activities

Exposure to fluoride aggravates the impairment in learning and memory and neuropathological lesions in mice carrying the APP/PS1 double-transgenic mutation

Contact Info

Product

Resources

About