Fluoride modulates preosteoblasts viability and matrix metalloproteinases-2 and -9 activities

Abstract

This study evaluated the influence of fluoride on cell viability and activity of matrix metalloproteinases (MMP) -2 and -9 secreted by preosteoblasts. Preosteoblasts (MC3T3-E1 murine cell line) were cultured in MEM medium supplement with 10% Fetal Bovine Serum (FBS) and nucleosides/ribonucleosides without ascorbic acid. Adherent cells were treated with different concentrations of F (as sodium fluoride-NaF) in medium (5 x 10 -6 M, 10 -5 M, 10 -4 M and 10 -3 M) for 24, 48, 72 and 96 h at 37 o C, 5% CO 2 . Control cells were cultivated in MEM only. After each period, preosteoblast viability was assessed by MTT assay. MMP-2 and -9 activities were performed by gel zymography. Also, alkaline phosphatase (ALP) activity was quantified by colorimetry in all experimental groups. It was shown that cultured cells with the highest dose of F (10 -3 M) for 96 h decreased preosteoblast viability while lower doses of F did not alter it, when compared to untreated cells. No differences were observed in ALP activity among groups. Moreover, compared to control, the treatment of cells with F at low dose slightly increased MMP-2 and -9 activities after 24 h. It was concluded that F modulates preosteoblast viability in a dose-dependent manner and also may regulate extracellular matrix remodeling.

Pharmacological and toxicological effects of co-exposure of human gingival fibroblasts to silver nanoparticles and sodium fluoride

“…Several studies have shown that millimolar levels of F can induce apoptosis in many cell types, including hepatic cells, epithelial lung cells, human leukemia HL-60 cells, and ameloblast-lineage cells. [27][28][29][30] We have also shown that F increases apoptosis in hippocampal and osteoblast-like cells. 10 However, other mechanisms can be involved in F-mediated cell death, such as endoplasmic reticulum stress.…”

“…It has also been found that high concentrations of F resulted in the upregulation of MMP-2 and MMP-9 in pro-osteoblast cells. 30 However, it has been also suggested that an oral hygiene regimen based on amine/ stannous fluoride-containing toothpaste is effective in reducing plaque-associated gingivitis and gingival inflammation. 40 Of note, AgNPs cause inflammation in other cell types, such as liver cells both in vivo and in vitro.…”

Efeito do extrato de aroeira no processo de proliferação e mineralização de osteoblastos in vitro

“…Nesse trabalho também avaliamos a expressao gênica da MMP-2 em diversos períodos experimentais frente a utilização do extrato de aroeira. As MMPs contribuem tanto para a remodulação tecidual normal quanto patológica (SLOMPO et al, 2012). Os papéis fisiológicos das MMPs incluem a migração celular, a remodelação do tecido durante organogênese e crescimento, cicatrização de feridas, 6 Discussão 98 a angiogênese e formação do esmalte (HANNAS et al, 2007;TOCCHI & PARKS, 2013).…”

“…As MMPs pertencem à família de proteinases zinco dependentes neutras (SOUZA & LINE, 2002). Entre os representantes das MMPs encontram-se as chamadas gelatinases A ou MMP-2 (72 kDa) tipo colagenase IV e gelatinase B ou MMP-9 (92 kDa) que degrada especialmente o colágeno tipo IV e regula o remodelamento das membranas basais (MAKELA et al, 1994;SLOMPO et al, 2012).…”

“…As MMPs são sintetizadas e secretadas por algumas células como fibroblastos (SLOMPO et al, 2012), osteoblastos e odontoblastos para a matriz extracelular (PAGE-MCCAW et al, 2007 (KOROSTISHEVSKY et al, 2008;KIRSCH, 2012;ZHOU et al, 2012).…”