Fluorimetric detector and sensor for flow analysis made of light emitting diodes

Tymecki, Łukasz; Rejnis, Magdalena; Pokrzywnicka, Marta; Strzelak, Kamil; Koncki, Robert

doi:10.1016/j.aca.2012.01.029

Cited by 23 publications

(6 citation statements)

References 27 publications

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“…Koncki et al reported the use of LEDs for both absorption and fluorescence measurement. 25,26 The PEDD has been employed in the analysis of physiological samples, [27][28][29][30][31][32][33][34][35][36][37][38][39][40] drink and food samples [41][42][43][44] and environmental samples. 45 There are also reports of dual analysis employing PEDDs, for example, multicommutated flow (MCFA) system for the determination of activity of acid phosphate and alkaline phosphatase, 46 sequential injection (SI) system for quantitative measurement of sucrose and phosphate, 47 MCFA system for determination of phosphate and calcium, 48 MCFA system for total protein and creatinine analysis 49 and MCFA system for investigation of iron-transferrin and apotransferrin speciation in human serum.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Colorimetric Measurements of Antioxidant Capacity by Flow Injection Analysis with Paired Emitter Detector Diode

et al. 2019

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An effective flow injection analysis (FIA) system employing paired emitter detector diode (PEDD) for simultaneous dual antioxidant assays is proposed. The total antioxidant capacity (TAC) was measured using the 2,2′-azino-bis(3ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay in parallel with the ferric reducing antioxidant power (FRAP) assay. Ascorbic acid was used as the reference antioxidant and the TAC value reported as ascorbic acid equivalent (AAE). A 10port valve furnished with two injection loops allowed the sample from one loop to react with the ABTS reagent and be detected by one PEDD. At the same time, the sample from the second loop reacted with the FRAP reagent and is detected at the second PEDD. A pair of red light emitting diodes (634 nm) was employed for both PEDDs. The linearity range was 10 to 50 μM ascorbic acid, with limit of detection of 3.18 and 4.37 μM, and precision of 2.41 and 2.15% RSD (for 30 μM ascorbic acid, n = 10) for the ABTS and FRAP assay, respectively. Sample throughput of 90 samples/h was achieved. The method was applied to the measurement of TAC of commercial fruit juice, instant tea products and vitamin C tablets. The observed AAE values were in good agreement with those obtained using batch methods with a spectrophotometer.

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Section: Introductionmentioning

confidence: 99%

Simultaneous Colorimetric Measurements of Antioxidant Capacity by Flow Injection Analysis with Paired Emitter Detector Diode

et al. 2019

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“…The design of a fluorometer for the determination of inten sively fluorescent quinine based on three LEDs with out additional optoelectronic components was devel oped in [12], and the application area of the scheme was extended and described in detail in [13]. The com bination of LEDs for excitation and registration of the fluorescence signal allows extreme simplification of the fluorometer scheme eliminating primary and sec ondary filters that decrease the intensity of the excita tion and emission signals, decrease its dimensions, and provide the possibility of an in house detector design [13]. Nevertheless, the use of low power (100-200 mW) LEDs for signal excitation and registration allowed detection only of highly fluorescent com pounds (quinine, riboflavin) at high concentrations.…”

Section: Introductionmentioning

confidence: 99%

A fluorometer on the basis of powerful light emitting diodes for determination of the chlorophyll “a” concentration

Povazhnyi

2014

Oceanology

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A simple fluorometer for determination of the chlorophyll "a" (chl "a") concentration in an extract based on bright light emitting diodes (LEDs) in the blue and IR spectrum range used as a continuous excitation light source and light detector, respectively, has been described. Results of calibration and valida tion of the system's signal with the help of extracts with different chl "a" concentrations are presented.

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“…Such approach is possible because LEDs are found to be partially selective detectors able to detect fluorescence emission with negligible effects from the exciting light. Until now, two and three LED-based fluorimetric detectors have been successfully developed for determination of calcium [ 25 , 26 ] and phosphate [ 14 , 15 ] ions, quinine [ 17 ], vitamin B [ 27 ], and proteins [ 20 ]. Only recently, turbidimetric [ 28 – 31 ] and nephelometric [ 30 , 31 ] detectors operating according to PEDD principle have been reported.…”

Section: Introductionmentioning

confidence: 99%

“…Another example is an optoelectronic flow-through redox sensor based on chemosensitive Prussian blue film, useful for determination of ascorbic acid and hydrogen peroxide [ 36 ]. Finally, also the first prototypes of fluorimetric PEDD-based sensors dedicated for the detection of riboflavin [ 27 ] and oxygen [ 37 ] have been demonstrated.…”

Section: Introductionmentioning

confidence: 99%

Towards optoelectronic urea biosensors

2015

Self Cite

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Integration of immobilized enzymes with light-emitting diodes (LEDs) leads to the development of optoelectronic enzyme-based biosensors. In this work, urease, used as a model enzyme, immobilized in the form of an open-tubular microbioreactor or biosensing membrane that has been integrated with two red LEDs. It forms complete, fiberless, miniaturized, and extremely economic biooptoelectronic devices useful for nonstationary measurements under flow analysis conditions. Both enzyme-based biodevices, operating according to the paired emitter detector diode (PEDD) principle, allow relatively fast, highly sensitive, and well-reproducible urea detection in the millimolar range of concentrations. Potential analytical applications of the developed urea bioPEDDs have been announced. Both presented constructions will be easily adapted for the development of other optoelectronic biosensors exploring various enzyme-based schemes of biodetection.Electronic supplementary materialThe online version of this article (doi:10.1007/s00216-014-8434-z) contains supplementary material, which is available to authorized users.

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Fluorimetric detector and sensor for flow analysis made of light emitting diodes

Cited by 23 publications

References 27 publications

Simultaneous Colorimetric Measurements of Antioxidant Capacity by Flow Injection Analysis with Paired Emitter Detector Diode

Simultaneous Colorimetric Measurements of Antioxidant Capacity by Flow Injection Analysis with Paired Emitter Detector Diode

A fluorometer on the basis of powerful light emitting diodes for determination of the chlorophyll “a” concentration

Towards optoelectronic urea biosensors

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