Fluorimetric determination of activity and isoenzyme composition of N-acetyl-β-D-hexosaminidase in seminal plasma of fertile men and infertile patients with secretory azoospermia

Tassi, C; Angelini, Antonio; Beccari, Tommaso; Capodicasa, Enrico

doi:10.1515/cclm.2006.154

Cited by 4 publications

(2 citation statements)

References 22 publications

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“…Sperm contain the enzyme N-acetyl-beta-D hexosaminidase type B or HEX-B, which cleaves the ZP-glycoproteins of the zona pellucida of the ovum. In vitro employment of ZP-glycoprotein analogues to block HEX-B of rodent and bovine semen had 98% efficacy regarding fertilization failure, 108 while in vivo, the corresponding efficiency rates were approximately 90%, 109 without overt adverse effects and restoration of fertility one week after discontinuation of treatment.…”

Section: Disruption Of Sperm Transport In the Epididymismentioning

confidence: 99%

Male contraception: a clinically-oriented review

Kanakis

Goulis

2015

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Despite the variety of available female contraceptive methods, many pregnancies (~50%) are still undesired. Many men (>60%) want to participate equally with their partner in family planning; however, male contraceptive methods (MCMs) account for only 14% of those used worldwide and no pharmaceutical MCM is available so far. The only two MCMs currently available are condoms, which despite protecting against sexually transmitted diseases have high failure rates (~19%), and vasectomy, which though very efficient (99%) is poorly reversible (<50%). Among MCMs under investigation, male hormonal contraceptives (MHCs) are those that have come closest to commercialization. The action of MHCs relies on the disruption of spermatogenesis that exogenous androgen administration evokes by suppressing the hypophyseal-gonadal axis. Various regimens of androgens as monotherapy or in combination with progestins have been tested in clinical trials achieving a Pearl Index <1.0 (equal to that of the female oral contraceptive pill); however, concerns regarding the variable response rates observed (non-responders: 5-20%), the impracticality of parenteral administration and long-term prostate-associated or cardiovascular morbidity have deflected the interest of the pharmaceutical industry from further research. Non-hormonal contraception methods may be, at least theoretically, more specific by selectively disrupting spermatogenesis and sperm transport or fertilizing ability. Nevertheless, only a few have been tested in clinical trials (Reversible Inhibition of Sperm Under Guidance, RISUG, and Intra Vas Plugs); most of them are still in pre-clinical development or have been abandoned due to toxicity (gossypol). Consequently, until a reliable, safe and practical MCM is developed, women will continue to bear most of the contraception burden.

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Section: Disruption Of Sperm Transport In the Epididymismentioning

confidence: 99%

Male contraception: a clinically-oriented review

Kanakis

Goulis

2015

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“…[17][18][19] Different and expensive techniques have been carried out for the evaluation of NAG isoenzymes in urine. [17][18][19][20][21][22][23] Noteworthy, all NAG isoenzymes have activity toward 4-methylumbelliferyl-2-acetamido-2-deoxy-β-D-glucopyranoside (MUG) substrate. The presence of α subunit enables NAG A to hydrolyze the GM2 ganglioside and the fluorogenic substrate 4-methylumbelliferyl-2-acetamido-2-deoxy-β-Dglucopyranoside-6-sulfate (MUGS) while NAG B isoform does not hydrolyze the MUGS substrate.…”

Section: Introductionmentioning

confidence: 99%

Isoenzyme A and UrinaryN-Acetyl-β-D-Glucosaminidase Activity in Normal Pregnancy

2011

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Objectives: Urinary N-acetyl-β-D-glucosaminidase (NAG) activity has been found to increase during normal uncomplicated pregnancy and such behavior could limit the diagnostic value of this enzyme for detection of subclinical tubular injury. The aim of this study was to evaluate urinary NAG activity and isoenzyme A in normal pregnant women at 30th week of pregnancy and in healthy women, to discriminate between physiological and lesional enzymuria. Design and methods: Enzyme activities in first morning fasting urine samples from 20 nonpregnant control and 20 normal pregnant women at 30th gestational week were evaluated by fluorometric methods. Results: Both total and isoenzyme A activity was significantly higher ( p < 0.01) in urines of normal pregnant women compared with control urines, whereas ratio between these two parameters was significantly lower ( p < 0.001). Conclusions: The increase of urinary NAG activity during normal uncomplicated pregnancy appears to be characterized by a prevalent increase in isoenzyme A form, a finding associated with functional (not lesional) enzymuria. The fluorometric assays may represent a simple and rapid method to evaluate whether increase in urinary NAG activity represents a renal physiological adaptation during pregnancy.

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Overview on the clinical presentation and indications: Part B

Erasmus

2021

Herbal Medicine in Andrology

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Fluorimetric determination of activity and isoenzyme composition of N-acetyl-β-D-hexosaminidase in seminal plasma of fertile men and infertile patients with secretory azoospermia

Cited by 4 publications

References 22 publications

Male contraception: a clinically-oriented review

Male contraception: a clinically-oriented review

Isoenzyme A and UrinaryN-Acetyl-β-D-Glucosaminidase Activity in Normal Pregnancy

Overview on the clinical presentation and indications: Part B

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