Fluorimetric screening assay for protein carbonyl evaluation in biological samples

Stocker, Pierre; Ricquebourg, Emilie; Vidal, Nicolás; Villard, Claude; Lafitte, Daniel; Sellami, Lyna; Pietri, Sylvia

doi:10.1016/j.ab.2015.04.021

Cited by 19 publications

(11 citation statements)

References 50 publications

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“…Our study also detected abundant intracellular and extracellular ROS generation after DVDMS-PDT treatment. The oxidative damage, improving the content of carbonyl protein, occurred in close proximity to the site where ROS were formed 56 57 58 . Since we have demonstrated that DVDMS presented a strong affinity to BSA, the generated ROS during the PDT could damage proteins and eliminate their biological function.…”

Section: Discussionmentioning

confidence: 99%

Interaction and oxidative damage of DVDMS to BSA: a study on the mechanism of photodynamic therapy-induced cell death

Wang

et al. 2017

Sci Rep

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Photodynamic therapy (PDT) is a promising method for neoplastic and nonneoplastic diseases. In this study, we utilized sinoporphyrin sodium (DVDMS) as a sensitizer combined with light to investigate its cytotoxic effect on different cell lines. For this purpose, we chose bovine serum albumin (BSA) as a model to explore the mechanism of PDT-induced cell death at a molecular level. Our findings indicated that the combined treatment significantly suppressed cell survival. Fluorescence spectroscopy revealed a strong interaction between DVDMS and BSA molecules in aqueous solution, affecting DVDMS’ targeting distribution and metabolism. Spectroscopic analysis and carbonyl content detection indicated that DVDMS-PDT significantly enhanced the damage of BSA at a higher extent than Photofrin II-PDT under similar experimental conditions. Our observations were consistent with the cytotoxicity results. Excessive reactive oxygen species (ROS) were induced by the synergy effect of the sensitizer and light, which played an important role in damaging BSA and tumor cells. These results suggested that the interaction and oxidative damage of protein molecules by DVDMS were the main reasons to cell death and constitute a valuable reference for future DVDMS-PDT investigations.

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Section: Discussionmentioning

confidence: 99%

Interaction and oxidative damage of DVDMS to BSA: a study on the mechanism of photodynamic therapy-induced cell death

Wang

et al. 2017

Sci Rep

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“…Glycogen and triglyceride contents were assayed in liver tissues using commercial assay kits (Caymann Chemical). Plasma protein carbonyls were assayed using a fluorimetric method based on derivatization with 7-hydrazino-4nitrobenzo-2,1,3-oxadiazole (NBDH; Sigma−Aldrich) according to Stocker et al (2015). For details see the Supplementary methods section…”

Section: Plasma Glucose Insulin and Lipid Metabolism And Protein Camentioning

confidence: 99%

Antidiabetic, antioxidant and anti inflammatory properties of water and n-butanol soluble extracts from Saharian Anvillea radiata in high-fat-diet fed mice

Kandouli

Cassien

Mercier

et al. 2017

Journal of Ethnopharmacology

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“…-. Rings were incubated for 90 min with excess NADPH (1 mM) and the protective effect of eight PPNs (200 µM) on protein oxidation was evaluated by assaying tissue protein carbonyl levels with an improved procedure [62]. Incubation medium contained diethyldithiocarbamate (DTC; 15 µM) to inactivate intracellular SOD [63].…”

Section: No-mediated Vasorelaxant Potency and Antioxidant Properties mentioning

confidence: 99%

“…Protein carbonyls content was determined in ring tissues homogenates using the probe 7-hydrazino-4-nitrobenzo-2,1,3-oxadiazole (NBDH, Sigma-Aldrich) which forms highly fluorescent derivatives with several aldehydes via hydrazone formation [62]. Homogenates were 38 prepared by quickly homogenizing 0.1 g of fresh aortic tissue powdered in liquid N 2 in nine bulks of a 1.15% KCl ice-cold solution.…”

Section: Formation Of Ppns Spin Adducts and Their Epr Detection In Amentioning

confidence: 99%

On the vasoprotective mechanisms underlying novel β-phosphorylated nitrones: Focus on free radical characterization, scavenging and NO-donation in a biological model of oxidative stress

Cassien

Petrocchi

Thétiot-Laurent

et al. 2016

European Journal of Medicinal Chemistry

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A series of new hybrid 2-(diethoxyphosphoryl)-N-(benzylidene)propan-2-amine oxide derivatives with different aromatic substitution (PPNs) were synthesized. These molecules were evaluated for their EPR spin trapping potential and NO donation properties in vitro, their cytotoxicity and on precontracted rat aortic rings. A subfamily of the new PPNs featured an antioxidant moiety occurring in natural phenolic acids. From the experimental screening of these hydroxyphenyl-and methoxyphenyl-substituted PPNs, biocompatible nitrones 4d, 4g−i deriving from caffeic, gallic, ferulic and sinapic acids, which combined improved EPR probing of ROS formation, vasorelaxant action and antioxidant potency, might be potential drug candidate alternatives to PBN and its analogues.

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Fluorimetric screening assay for protein carbonyl evaluation in biological samples

Cited by 19 publications

References 50 publications

Interaction and oxidative damage of DVDMS to BSA: a study on the mechanism of photodynamic therapy-induced cell death

Interaction and oxidative damage of DVDMS to BSA: a study on the mechanism of photodynamic therapy-induced cell death

Antidiabetic, antioxidant and anti inflammatory properties of water and n-butanol soluble extracts from Saharian Anvillea radiata in high-fat-diet fed mice

On the vasoprotective mechanisms underlying novel β-phosphorylated nitrones: Focus on free radical characterization, scavenging and NO-donation in a biological model of oxidative stress

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