Fluorometric enzymatic autoindicating biosensor for H2O2 determination based on modified catalase

Ortega, Estefania; Marcos, Susana de; Galbán, Javier

doi:10.1016/j.bios.2012.08.001

Cited by 23 publications

(19 citation statements)

References 25 publications

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“…The sensor works steadily over 40 days (one daily measurement and storage in deionized water), without exhibiting any loss in sensitivity, recovery or response characteristics. Ortega et al [38] also proposed reversible optical biosensors based on an O2-sensitive ruthenium complex for continuous monitoring of H2O2. Unlike in the previous papers, one bpy ligand of the complex was substituted by 4-methyl-4-carboxy-2,2′-bipyridine to covalently link the complex to catalase (Cat-Ru).…”

Section: Sensors Sensing Membranes and Films For H 2 O 2 Detectionmentioning

confidence: 99%

New Nanomaterials and Luminescent Optical Sensors for Detection of Hydrogen Peroxide

2015

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Accurate methods that can continuously detect low concentrations of hydrogen peroxide (H2O2) have a huge application potential in biological, pharmaceutical, clinical and environmental analysis. Luminescent probes and nanomaterials are used for fabrication of sensors for H2O2 that can be applied for these purposes. In contrast to previous reviews focusing on the chemical design of molecular probes for H2O2, this mini-review highlights the latest luminescent nanoparticular materials and new luminescent optical sensors for H2O2 in terms of the nanomaterial composition and luminescent receptor used in the sensors. The nanomaterial section is subdivided into schemes based on gold nanoparticles, polymeric nanoparticles with embedded enzymes, probes showing aggregation-induced emission enhancement, quantum dots, lanthanide-based nanoparticles and carbon based nanomaterials, respectively. Moreover, the sensors are ordered according to the type of luminescent receptor used within the sensor membranes. Among them are lanthanide complexes, metal-ligand complexes, oxidic nanoparticles and organic dyes. Further, the optical sensors are confined to those that are capable to monitor the concentration of H2O2 in a sample over time or are reusable. Optical sensors responding to gaseous H2O2 are not covered. All nanomaterials and sensors are characterized with respect to the analytical reaction towards H2O2, limit of detection (LOD), analytical range, electrolyte, pH and response time/incubation time. Applications to real samples are given. Finally, we assess OPEN ACCESSChemosensors 2015, 3 254 the suitability of the nanomaterials to be used in membrane-based sensors and discuss future trends and perspectives of these sensors in biomedical research.

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Section: Sensors Sensing Membranes and Films For H 2 O 2 Detectionmentioning

confidence: 99%

New Nanomaterials and Luminescent Optical Sensors for Detection of Hydrogen Peroxide

2015

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“…A decrease or increase in fluorescence intensity or blue or red shifts may be connected with changes on the bioreceptor surface. This technique is commonly used for fluorescence investigations of biosensors containing an enzyme in its bio-sensitive layer [19,[53][54][55][56].…”

Section: Change In Fluorescence Intensitymentioning

confidence: 99%

“…Hydrolases such as acetylcholinesterase (AChE) and organophoshorous hydrolase (OPH) and its fluorescence properties were used in the defense industry to detect toxic warfare agents [19]. The biosensor based on cellobiose dehydrogenase (CDH) is used both in industry and environmental protection [54]. Biosensors containing nitrate reductases (NR), phosphatases, and ureases [15] were used in environmental research, like tyrosinases for detecting phenols and catechols in food and pharmaceuticals, as well as in clinical and environmental samples [16].…”

Section: Enzymes Used In Biosensorsmentioning

confidence: 99%

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Enzyme-Based Fluorescent Biosensors and Their Environmental, Clinical and Industrial Applications

Kłos-Witkowska¹

2015

Pol. J. Environ. Stud.

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The last two decades has seen a significant increase in interest in biosensors. According to the Web of Science database, the number of scientific publications over the past 20 years increased from 309 in 1993 up to 3,467 in 2013 (Fig. 1).The demand for developing new detection methods, increasing interest in visualization techniques [1][2][3], emphasis placed on new drug discovery programs [4], diagnostic test development [5][6][7], and attempts to explain cellular action mechanisms and to trace a cell's metabolism pathway [7,8], and the possibility of using biosensors in numerous fields of application, (for example: medicine [9-11], environmental protection [12-14, 16-17], food industry [18], and defense industry [19]) has results in an increasing number of research projects targeted at biosensor design and fabrication. Biosensors used in environmental monitoring measure the toxicity effect based on the detection of a chemical compound or compound group by selective recognition of a biomolecule in the receptor layer and then by detection of a signal after passing through the transducer layer [16].The application of fluorescence biosensors in environmental protection applications [20][21][22][23][24][25], medical diagnostics [26][27][28][29][30][31][32][33][34][35], and industries [36][37][38][39][40][41][42][43][44][45] also is growing.Pol. J. Environ. Stud. Vol. 24, No. 1 (2015) AbstractEnzyme-based fluorescence biosensors and their applications in environmental protection, medicine, and industry are described. Biosensors used in environmental protection measure toxicity effects. A chemical compound or group of compounds is detected by the recognition of molecules in the receptor layer and then by detecting a signal passing through the transducer layer. Biosensors are classified according to the transduction method. Special emphasis is placed on optical biosensors, especially fluorescent biosensors, and such measurement techniques as FRET (Fröster resonance energy transfer), FLIM (fluorescence lifetime imaging), FCS (fluorescence correlation spectroscopy), and changes in fluorescence intensity. The phenomenon of fluorescence in biosensors and the selection of appropriate methods are described. The use of enzymes in the receptor layer and enzyme classification according to its category and functions used for analyte detection are presented. The fluorescence properties of enzymes resulting from possessing such cofactors as flavin or heme (prosthetic) groups are discussed. Several methods for enzyme immobilization, namely entrapment, adsorption, covalent immobilization, cross linking, and affinity interaction are described, and the use of enzymatic fluorescence biosensors in the detection of analytes is presented.

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“…There are several enzymes that can be used for biosensors development such as catalase [1], cholesterol oxidase [2], myoglobin [3], glucose oxidase [4], bilirubin oxidase [5], laccase [6] and others. Horseradish peroxidase (HRP) is one of enzyme that used in electrochemistry as biosensor [7] and biofuel cell [8], as decolorization agent [10], immunoassay [11], biodegradation [12] and synthesis of polyaniline [13].…”

Section: Introductionmentioning

confidence: 99%

Biosensor H2O2 by Using Immobilized Horseradish Peroxidase Glutaraldehyde on Carbon Polyaniline Nanofiber Composite

Rizarullah¹,

Suryani²,

Ambarsari³

et al. 2016

Enz Eng

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An enzymatic biosensor has been developed for detection of hydrogen peroxide with immobilized Horseradish peroxidase (HRP). HRP was immobilized by using glutaraldehyde (GA) that cross linked with modified polyaniline (PANI) as mediator to improve electron transfer. Modified carbon paste electrodes (MCPE) PANI was more effective during electron transfer compared to carbon paste electrodes (CPE). Cyclic voltammetry method (VC) was used to determine the electrochemical properties of the modified electrode substrate to produce redox reactions. The effect of pH and temperature were analyzed by cyclic voltammetry. The optimum performance of HRP/GA/PANI was at pH 7 and 50°C. Kinetics parameters HRP enzyme were determined in optimum condition. The Michaelis-Menten constant (Km) value and current maximum (I max ) have been obtained as 1.71 mM and 0.29 mA.

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Fluorometric enzymatic autoindicating biosensor for H2O2 determination based on modified catalase

Cited by 23 publications

References 25 publications

New Nanomaterials and Luminescent Optical Sensors for Detection of Hydrogen Peroxide

New Nanomaterials and Luminescent Optical Sensors for Detection of Hydrogen Peroxide

Enzyme-Based Fluorescent Biosensors and Their Environmental, Clinical and Industrial Applications

Biosensor H2O2 by Using Immobilized Horseradish Peroxidase Glutaraldehyde on Carbon Polyaniline Nanofiber Composite

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