Fluorometric Imaging for Early Diagnosis and Prognosis of Rheumatoid Arthritis

Lee, Jeong Heon; Jung, Sang Youn; Park, G. Kate; Bao, Kai; Hyun, Hoon; Fakhri, Georges El; Choi, Hak

doi:10.1002/advs.201902267

Cited by 15 publications

(6 citation statements)

References 37 publications

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“…First, intraoperative NIR fluorescence imaging can differentiate the tumors from nearby normal tissues by visualizing them separately with two different colors. Since the bone-targeted NIR fluorophores are mostly 800 nm emitting, the combination of 700 nm or over 1,000 nm emitting fluorophores targeting other tissues, including cartilage [ 50 ], tendon [ 27 ], nerve [ 51 ], vasculature [ 25 ], and inflammation [ 28 ], enables real-time multispectral imaging and/or the prognostic evaluation of bone metastasis. Second, the photothermal effect of P800SO3-PEG with the features of noninvasiveness and high selectivity makes it useful as a promising adjunct and/or alternative technique to traditional cancer treatments [ 52 , 53 ].…”

Section: Discussionmentioning

confidence: 99%

P800SO3-PEG: a renal clearable bone-targeted fluorophore for theranostic imaging

et al. 2022

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Background Due to the deep tissue penetration and reduced scattering, NIR-II fluorescence imaging is advantageous over conventional visible and NIR-I fluorescence imaging for the detection of bone growth, metabolism, metastasis, and other bone-related diseases. Methods Bone-targeted heptamethine cyanine fluorophores were synthesized by substituting the meso-carbon with a sulfur atom, resulting in a bathochromic shift and increased fluorescence intensity. The physicochemical, optical, and thermal stability of newly synthesized bone-targeted NIR fluorophores was performed in aqueous solvents. Calcium binding, bone-specific targeting, biodistribution, pharmacokinetics, and 2D and 3D NIR imaging were performed in animal models. Results The newly synthesized S-substituted heptamethine fluorophores demonstrated a high affinity for hydroxyapatite and calcium phosphate, which improved bone-specific targeting with signal-background ratios > 3.5. Particularly, P800SO3-PEG showed minimum nonspecific uptake, and most unbound molecules were excreted into the urinary bladder. Histological analyses demonstrated that P800SO3-PEG remained stable in the bone for over two weeks and was incorporated into bone matrices. Interestingly, the flexible thiol ethylene glycol linker on P800SO3-PEG induced a promising photothermal effect upon NIR laser irradiation, demonstrating potential theranostic imaging. Conclusions P800SO3-PEG shows a high affinity for bone tissues, deeper tissue imaging capabilities, minimum nonspecific uptake in the major organs, and photothermal effect upon laser irradiation, making it optimal for bone-targeted theranostic imaging.

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Section: Discussionmentioning

confidence: 99%

P800SO3-PEG: a renal clearable bone-targeted fluorophore for theranostic imaging

et al. 2022

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“…NIR uorescence imaging systems NIR-I images were obtained using the K-FLARE imaging system as described previously in detail [22][23][24][25].…”

Section: Calcium Salts Binding Assaymentioning

confidence: 99%

Renal clearable bone-targeted contrast agents for theranostic imaging

Wang

Kang

Dinh

et al. 2022

Preprint

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Background: Due to the deep tissue penetration and reduced scattering, NIR-II fluorescence imaging is advantageous over conventional visible and NIR-I fluorescence imaging for the detection of bone growth, metabolism, metastasis, and other bone-related diseases. Methods: Here, we designed bone-targeted heptamethine cyanine fluorophores by substituting the meso-carbon with a sulfur atom, resulting in a bathochromic shift and increased fluorescence intensity, which together enables NIR-II tail imaging. Results: The newly synthesized S-substituted heptamethine fluorophores demonstrated a high affinity for hydroxyapatite and calcium phosphate, which improved bone-specific targeting with signal-background ratios >3.5. Particularly, P800SO3-PEG showed minimum nonspecific uptake, and most unbound molecules were excreted into the urinary bladder. Histological analyses demonstrated that P800SO3-PEG remained stable in the bone for over two weeks and was incorporated into bone matrices. Interestingly, the flexible thiol ethylene glycol linker on P800SO3-PEG induced a promising photothermal effect, making this bone-targeted agent a potential theranostic imaging agent.Conclusions: P800SO3-PEG shows a high affinity for bone tissues, minimum nonspecific uptake in the major organs, and photothermal effect upon laser irradiation, making it promising as a targeted NIR theranostic agent for bone diseases.

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“…Recently, nanomaterials have been delicately designed and constructed for highly efficient theranostics of RA and other chronic diseases. − In particular, inherent scavenging properties of specific nanomaterials have the potential to revolutionize therapeutic approaches to RA. , Nanozymes, referring to nanomaterials with enzyme-mimicking capability, have been adopted as ROS scavengers for the anti-inflammation treatment of RA. − Despite the favorable efficiency reported in previous studies, , the ROS scavenging capability of nanozymes might be insufficient to inhibit the OCs activation and potential bone destruction. Alternatively, nanozymes can be incorporated with other functional components to construct nanoplatforms for more efficient and precise theranostics of RA .…”

Section: Introductionmentioning

confidence: 99%

Multifunctional Janus Nanoplatform for Efficiently Synergistic Theranostics of Rheumatoid Arthritis

Liu

Chen

et al. 2023

ACS Nano

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Progress has been made in the application of nanomedicine in rheumatoid arthritis (RA) treatment. However, the whole process of monitoring and treatment of RA remains a formidable challenge due to the complexity of the chronic autoimmune disease. In this study, we develop a Janus nanoplatform (denoted as Janus-CPS) composed of CeO2–Pt nanozyme subunit on one side and periodic mesoporous organosilica (PMO) subunit on another side for simultaneous early diagnosis and synergistic therapy of RA. The Janus nanostructure, which enables more active sites to be exposed, enhances the reactive oxygen species scavenging capability of CeO2–Pt nanozyme subunit as compared to their core–shell counterpart. Furthermore, micheliolide (MCL), an extracted compound from natural plants with anti-osteoclastogenesis effects, is loaded into the mesopores of PMO subunit to synergize with the anti-inflammation effect of nanozymes for efficient RA treatment, which has been demonstrated by in vitro cellular experiments and in vivo collagen-induced arthritis (CIA) model. In addition, by taking advantage of the second near-infrared window (NIR-II) fluorescent imaging, indocyanine green (ICG)-loaded Janus-CPS exhibits desirable effectiveness in detecting RA lesions at a very early stage. It is anticipated that such a Janus nanoplatform may offer an alternative strategy of functional integration for versatile theranostics.

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Fluorometric Imaging for Early Diagnosis and Prognosis of Rheumatoid Arthritis

Cited by 15 publications

References 37 publications

P800SO3-PEG: a renal clearable bone-targeted fluorophore for theranostic imaging

P800SO3-PEG: a renal clearable bone-targeted fluorophore for theranostic imaging

Renal clearable bone-targeted contrast agents for theranostic imaging

Multifunctional Janus Nanoplatform for Efficiently Synergistic Theranostics of Rheumatoid Arthritis

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