2006
DOI: 10.1073/pnas.0607095104
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Flux control and excess capacity in the enzymes of glycolysis and their relationship to flight metabolism inDrosophila melanogaster

Abstract: An important question in evolutionary and physiological genetics is how the control of flux-base phenotypes is distributed across the enzymes in a pathway. This control is often related to enzymespecific levels of activity that are reported to be in excess of that required for demand. In glycolysis, metabolic control is frequently considered vested in classical regulatory enzymes, each strongly displaced from equilibrium. Yet the contribution of individual steps to control is unclear. To assess enzyme-specific… Show more

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Cited by 70 publications
(69 citation statements)
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“…The P-element insertions used to generate the excision allele pairs were: Idh, Idh EP3729 ; Mdh2, Mdh2 EY01940 ; Gdh, Gdh KG00965 ; Men, Men BG02790 ; Hex-A (20817) [EP(X)352]; Hex-C, CG8079 KG09154 . Hex-A, Idh and Gpdh alleles have been described previously [44,46,47]. In each gene, we have an excision allele with impaired activity and an excision allele with normal activity.…”
Section: Materials and Methods (A) Lifespan Designmentioning
confidence: 99%
See 1 more Smart Citation
“…The P-element insertions used to generate the excision allele pairs were: Idh, Idh EP3729 ; Mdh2, Mdh2 EY01940 ; Gdh, Gdh KG00965 ; Men, Men BG02790 ; Hex-A (20817) [EP(X)352]; Hex-C, CG8079 KG09154 . Hex-A, Idh and Gpdh alleles have been described previously [44,46,47]. In each gene, we have an excision allele with impaired activity and an excision allele with normal activity.…”
Section: Materials and Methods (A) Lifespan Designmentioning
confidence: 99%
“…In each gene, we have an excision allele with impaired activity and an excision allele with normal activity. Enzyme activities were assayed as in earlier work [44,[46][47][48][49] and are shown in units of change in optical density per minute (DOD). The Men 9D allele is a perfect excision.…”
Section: Materials and Methods (A) Lifespan Designmentioning
confidence: 99%
“…There is a growing appreciation that this has resulted in geographical selection for many traits, especially those associated with the ability to withstand the stresses associated with overwintering in temperate populations (Schmidt et al, 2005;Schmidt and Paaby, 2008;Reaume and Sokolowski, 2006;Mitrovski and Hoffmann, 2001;Hoffmann et al, 2001;Umina et al, 2005). From an experimental standpoint, the genetic tools of D. melanogaster are unparalleled and methods are available to permanently knockout or modify activity in many genes (Bellen et al, 2004;Venken and Bellen, 2005) and to control metabolic function (Merritt et al, 2005;Merritt et al, 2006;Eanes et al, 2006;Eanes et al, 2008;Merritt et al, 2009). In addition, D. melanogaster has been a focal model in the study of energy partitioning and aging, where differential expression of the energyproducing and energy-consuming pathways has come under intense scrutiny as a response to dietary restriction (Gershman et al, 2007;Baker and Thummel, 2007).…”
Section: Introductionmentioning
confidence: 99%
“…The imprecise excision is frequently accompanied by loss of genomic sequences immediately flanking the starting P element, which became a tool to produce null mutations for genes whose 59 regulatory sequences were tagged with P insertions. In genetic screens to recover deficiencies from P imprecise excision, phenotypes associated with deletions extending into the flanking genes (Tsubota and Schedl 1986;Salz et al 1987), molecular detections of flanking genomic rearrangements (Zhang and Spradling 1993), or a combination of phenotypic selection and molecular detection (Mellerick et al 1992;Eanes et al 2006;Xu et al 2006;Hao et al 2007), were applied. Here, we characterized seven deletions generated by activating a nearby P element.…”
Section: T Ransposon Insertional Mutagenesis Has Beenmentioning
confidence: 99%