FNDC3B and BPGM Are Involved in Human Papillomavirus-Mediated Carcinogenesis of Cervical Cancer

Abstract

Human papillomavirus (HPV)-mediated cervical carcinogenesis is a multistep progressing from persistent infection, precancerous lesion to cervical cancer (CCa). Although molecular alterations driven by viral oncoproteins are necessary in cervical carcinogenesis, the key regulators behind the multistep process remain not well understood. It is pivotal to identify the key genes involved in the process for early diagnosis and treatment of this disease. Here we analyzed the mRNA expression profiles in cervical samples including normal, cervical intraepithelial neoplasia (CIN), and CCa. A co-expression network was constructed using weighted gene co-expression network analysis (WGCNA) to reveal the crucial modules in the dynamic process from HPV infection to CCa development. Furthermore, the differentially expressed genes (DEGs) that could distinguish all stages of progression of CCa were screened. The key genes involved in HPV-CCa were identified. It was found that the genes involved in DNA replication/repair and cell cycle were upregulated in CIN compared with normal control, and sustained in CCa, accompanied by substantial metabolic shifts. We found that upregulated fibronectin type III domain-containing 3B (FNDC3B) and downregulated bisphosphoglycerate mutase (BPGM) could differentiate all stages of CCa progression. In patients with CCa, a higher expression of FNDC3B or lower expression of BPGM was closely correlated with a shorter overall survival (OS) and disease-free survival (DFS). A receiver operating characteristic (ROC) analysis of CIN and CCa showed that FNDC3B had the highest sensitivity and specificity for predicting CCa development. Taken together, the current data showed that FNDC3B and BPGM were key genes involved in HPV-mediated transformation from normal epithelium to precancerous lesions and CCa.

Integrated genomic and transcriptomic analysis reveals the activation of PI3K signaling pathway in HPV-independent cervical cancers

“…Especially, FNDC3B was located and enriched in that area. Previous studies illustrated that FNDC3B abundance was correlated with the development and invasion in CCs [ 37 ], HNSCs [ 38 ] and gastric cancers [ 39 ], and it also predicted a poor prognosis [ 40 ]. Therefore, we hypothesized that FDNC3B may act as a surrogate pathogenic factor in PIK3CA wild-type HPV-ind CCs.…”

Bisphosphoglycerate mutase predicts myocardial dysfunction and adverse outcome in sepsis: an observational cohort study

“…Although BPGM plays a crucial role in modulating 2,3-BPG concentrations, with indirect effects on the glycolytic pathway, current research primarily focuses on its implications in oncology. Notably, in malignancies such as hepatocellular carcinoma and cervical cancer, BPGM espression is higher in tumor cells than in normal cells [ 23 , 24 ]. Recent research by Brendon et al identified BPGM as a potential prognostic marker in sepsis by associating high BPGM expression level with a poor prognosis in patients with the Mars1 molecular phenotype [ 25 ].…”