Focus Floating Microscopy: "Gold Standard" for Cutaneous Borreliosis?

Eisendle, Klaus; Grabner, Tanja; Zelger, Bernhard

doi:10.1309/m274-75u1-6356-w058

Cited by 30 publications

(69 citation statements)

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“…34 We named this procedure focus-floating microscopy (FFM). In cases in which abundant B burgdorferi were detected by FFM were usually positive by PCR and when fewer organisms were detected by FFM, specimens were more likely to be negative by PCR.…”

Section: For Editorial Comment See Page 662mentioning

confidence: 99%

“…34 Briefly, we used a polyclonal rabbit antibody (Acris BP1002, derived from immunization with whole-cell B burgdorferi preparations [strain B31, ATCC 35210; American Type Culture Collection, Manassas, Virginia] reacting with 83-kD and 41-kD flagellin, 32-kD OspB, and 31-kD OspA antigens and their fragments in Western blots, with cross-reaction to Treponema pallidum, Borrelia hermsii, and Borrelia parkeri) at a dilution of 1:2000, with an autoclave antigen retrieval time of 30 minutes in a sodium citrate buffer (pH 6.0-6.1) and an incubation time of 30 minutes at 37°C.…”

Section: Immunohistochemical Analysismentioning

confidence: 99%

“…34 Focus- floating microscopy scans through the sections in 2 planes: horizontally in a serpentinelike pattern, as in routine cytologic examination, and, simultaneously, vertically by focusing through the thickness of the cut (usually 3-4 µm) at an original magnification of ϫ200 to ϫ400. This holoscopic approach allows for the detection of B burgdorferi (diameter of 0.2 µm, compared with 2.0 µm for collagen bundles), which pass through the section at various angles and accordingly may appear as undulated, comma-to dot-shaped forms.…”

Section: Focus-floating Microscopymentioning

confidence: 99%

See 2 more Smart Citations

Possible Role of Borreliaburgdorferi Sensu Lato Infection in Lichen Sclerosus

Eisendle¹,

Grabner²,

Kutzner³

et al. 2008

Arch Dermatol

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Section: For Editorial Comment See Page 662mentioning

confidence: 99%

Section: Immunohistochemical Analysismentioning

confidence: 99%

Section: Focus-floating Microscopymentioning

confidence: 99%

See 1 more Smart Citation

Possible Role of Borreliaburgdorferi Sensu Lato Infection in Lichen Sclerosus

Eisendle¹,

Grabner²,

Kutzner³

et al. 2008

Arch Dermatol

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“…Es fehlen jedoch Vergleichsuntersuchungen mit PCR und Anzucht von Borrelien [13]. Die Ergebnisse konnten bisher von anderen Arbeitsgruppen nicht bestä-tigt werden.…”

Section: Labordiagnostikunclassified

Variabilität der kutanen Lyme-Borreliose

Hofmann

2012

Hautarzt

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Lyme borreliosis can affect almost all human organs. Erythema migrans is the first and most frequent manifestation in 80-90% of patients in the early stage of localized skin infection. Besides the typical clinical appearance, many atypical variants can be observed. The solitary borrelial lymphocytoma is much less common and occurs mostly in children. Due to improvement in the early recognition of Lyme borreliosis, the diagnosis is made in the disseminated and late stage in only 10-20% of patients. Multiple erythemata migrantia indicating the hematogenous dissemination of B. burgdorferi remain frequently unrecognized. Late stages of infection feature chronic plasma-cell rich cutaneous inflammation and acrodermatitis chronica atrophicans in its edematous to atrophic forms. Cultivation or DNA detection of B. burgdorferi in skin biopsies are options to prove unusual skin manifestations. Serological detection of Borrelia-specific IgG- and IgM antibodies should be performed according to the two step protocol with ELISA and immunoassay according to the criteria of the MIQ 12. Serological tests have limited utility for follow-up. Antibiotic therapy is very effective if performed according to evidence-based protocols, such as the AWMF guidelines.

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“…burgdorferi. 67 In Manaus, 22 EM patients were analyzed through the technique described above and the presence of Borrelia burgdorferi was detected in five (22.7%) cases (Figure 3). 26 Although the PCR technique detects nucleic acid sequences of Borrelia, with high specificity, the sensitivity of this method is variable (20-81%).…”

Section: Diagnosismentioning

confidence: 99%

Borreliose de Lyme

Santos

Júnior

Ribeiro‐Rodrigues

et al. 2010

An. Bras. Dermatol.

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Abstract:Borreliosis is an infectious disease caused by spirochetes of the genus Borrelia. Lyme borreliosis, also known as Lyme disease, is a non-contagious infectious disease caused by spirochetes belonging to the complex Borrelia burgdorferi sensu lato and more often transmitted by the bite of infected ticks of the genus Ixodes.The disease is characterized by a varied clinical profile, which can trigger cutaneous, articular, neurological and cardiac manifestations. Key words: Keywords: Borrelia burgdorferi, Lyme disease, chronic Erythema migrans; Ixodes Resumo: As borrelioses constituem um grupo de doenças infecciosas causadas por espiroquetas do gêne-ro Borrelia. A borreliose de Lyme, também denominada doença de Lyme, é uma doença infecciosa, não contagiosa, causada por espiroquetas pertencentes ao complexo Borrelia burgdorferi Sensu Lato e transmitida, mais frequentemente, por picada de carrapatos do gênero Ixodes. A doença apresenta quadro clí-nico variado, podendo desencadear manifestações cutâneas, articulares, neurológicas e cardíacas.

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Focus Floating Microscopy: "Gold Standard" for Cutaneous Borreliosis?

Cited by 30 publications

References 0 publications

Possible Role of Borreliaburgdorferi Sensu Lato Infection in Lichen Sclerosus

Possible Role of Borreliaburgdorferi Sensu Lato Infection in Lichen Sclerosus

Variabilität der kutanen Lyme-Borreliose

Borreliose de Lyme

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