Focusing and sorting of multiple-sized beads and cells using low-aspect-ratio spiral microchannels

Thanormsridetchai, Attawut; Ketpun, Dettachai; Srituravanich, Werayut; Piyaviriyakul, Prapruddee; Sailasuta, Achariya; Jeamsaksiri, Wutthinan; Sripumkhai, Witsaroot; Pimpin, Alongkorn

doi:10.1007/s12206-017-1034-z

Cited by 15 publications

(16 citation statements)

References 23 publications

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“…At a flow rate of 8 mL/min (for diluted blood) and 800 µL/min (for whole blood); 83% capture efficiency was recorded. Thanormsridetchai et al developed a spiral microfluidic device for capturing of CTCs [131]. The device with five spiral microchannels (500 µm height, 130 µm width, 5.5 mm length) was injected with samples at a flow rate of 1.0 mL/min.…”

Section: Inertial Focusing Microchannel-based Isolationmentioning

confidence: 99%

Recent Advances in Microfluidic Platform for Physical and Immunological Detection and Capture of Circulating Tumor Cells

et al. 2022

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CTCs (circulating tumor cells) are well-known for their use in clinical trials for tumor diagnosis. Capturing and isolating these CTCs from whole blood samples has enormous benefits in cancer diagnosis and treatment. In general, various approaches are being used to separate malignant cells, including immunomagnets, macroscale filters, centrifuges, dielectrophoresis, and immunological approaches. These procedures, on the other hand, are time-consuming and necessitate multiple high-level operational protocols. In addition, considering their low efficiency and throughput, the processes of capturing and isolating CTCs face tremendous challenges. Meanwhile, recent advances in microfluidic devices promise unprecedented advantages for capturing and isolating CTCs with greater efficiency, sensitivity, selectivity and accuracy. In this regard, this review article focuses primarily on the various fabrication methodologies involved in microfluidic devices and techniques specifically used to capture and isolate CTCs using various physical and biological methods as well as their conceptual ideas, advantages and disadvantages.

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Section: Inertial Focusing Microchannel-based Isolationmentioning

confidence: 99%

Recent Advances in Microfluidic Platform for Physical and Immunological Detection and Capture of Circulating Tumor Cells

et al. 2022

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“…Both syringes were simultaneously empowered by a two-port programmable automatic syringe pump (F-100, Chemyx, Stafford, TA, USA). The optimal flow rate used in this study was at 1 mL·min −1 based upon the previous studies [ 19 , 20 , 21 ]. Data acquisitions were done with reverse light microscopy.…”

Section: Methodsmentioning

confidence: 99%

“…Most of the larger cells sized from 10 μm were focused and distributed through the outlets II and III of the microdevice. In contrast to small MCT cells, they were unfocused and dispersed through the outlets I to V. Notably, the sizes of individual MCT cells from each outlet were measured by fluorescence-activated cell sorting (FACS) size-based quantification [ 21 ].…”

Section: Figurementioning

confidence: 99%

“…In addition, our preliminary studies have substantially demonstrated the efficacy of our predesigned Archimedean spiral microchannel in the separation of multiple-sized polystyrene microspheres, intact canine blood cells and feline leukemic cells at the optimal flow rate of 1 mL·min −1 [ 19 , 20 ], and naturally-buoyant canine cutaneous mast cell tumor (MCT) cells harvested by fine-needle aspiration (FNA). In that study, the results indicated that our microdevice could efficiently isolate 15–25 μm terminally differentiated MCT cells from the smaller contaminated cells, including putative MCT cancer stem cells (<7 μm), which were unfocused.…”

Section: Introductory Backgroundmentioning

confidence: 99%

“…The sorted MCT cells were substantially harvested at the outlet III of the microdevice. Meanwhile, unfocused contaminants dispersed throughout the device and could be collected from all outlets [ 21 ].…”

Section: Introductory Backgroundmentioning

confidence: 99%

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The Viability of Single Cancer Cells after Exposure to Hydrodynamic Shear Stresses in a Spiral Microchannel: A Canine Cutaneous Mast Cell Tumor Model

et al. 2017

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Our laboratory has the fundamental responsibility to study cancer stem cells (CSC) in various models of human and animal neoplasms. However, the major impediments that spike our accomplishment are the lack of universal biomarkers and cellular heterogeneity. To cope with these restrictions, we have tried to apply the concept of single cell analysis, which has hitherto been recommended throughout the world as an imperative solution pack for resolving such dilemmas. Accordingly, our first step was to utilize a predesigned spiral microchannel fabricated by our laboratory to perform size-based single cell separation using mast cell tumor (MCT) cells as a model. However, the impact of hydrodynamic shear stresses (HSS) on mechanical cell injury and viability in a spiral microchannel has not been fully investigated so far. Intuitively, our computational fluid dynamics (CFD) simulation has strongly revealed the formations of fluid shear stress (FSS) and extensional fluid stress (EFS) in the sorting system. The panel of biomedical assays has also disclosed cell degeneration and necrosis in the model. Therefore, we have herein reported the combinatorically detrimental effect of FSS and EFS on the viability of MCT cells after sorting in our spiral microchannel, with discussion on the possibly pathogenic mechanisms of HSS-induced cell injury in the study model.

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Computational Models for Optimizing Particle Separation in Spiral Inertial Microfluidics: A Step Toward Enhanced Biosensing and Cell Sorting

Boland,

Yang,

Yin

et al. 2024

Advcd Theory and Sims

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Inertial microfluidics is essential for separating particles and cells, enabling numerous biomedical applications. Despite the simplicity of spiral microchannels, the lack of predictive models hampers real‐world applications, highlighting the need for cost‐effective computational tools. In this study, four novel data fitting models are developed using linear and power regression analyses to investigate how flow conditions influence particle behaviors within spiral microchannels. These models are rigorously tested under two different flow rates, focusing on a smaller particle representing Salmonella Typhimurium and a larger particle representing bacterial aggregates, aiming for effective separation and detection. A critical parameter, the sheath‐to‐sample flow rate ratio, is either interpolated or extrapolated using the microchannel's aspect ratios to predict particle separation. The models show strong agreement with experimental data, underscoring their predictability and efficiency. These insights suggest that further refinement of these models can significantly reduce research and development costs for advanced inertial microfluidic devices in biomedical applications. This work represents a crucial step towards establishing a robust computational framework, advancing inertial microfluidics towards practical biomedical applications.

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Focusing and sorting of multiple-sized beads and cells using low-aspect-ratio spiral microchannels

Cited by 15 publications

References 23 publications

Recent Advances in Microfluidic Platform for Physical and Immunological Detection and Capture of Circulating Tumor Cells

Recent Advances in Microfluidic Platform for Physical and Immunological Detection and Capture of Circulating Tumor Cells

The Viability of Single Cancer Cells after Exposure to Hydrodynamic Shear Stresses in a Spiral Microchannel: A Canine Cutaneous Mast Cell Tumor Model

Computational Models for Optimizing Particle Separation in Spiral Inertial Microfluidics: A Step Toward Enhanced Biosensing and Cell Sorting

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