Folate-Functionalized Mesoporous Silica Nanoparticles as a Liver Tumor-Targeted Drug Delivery System to Improve the Antitumor Effect of Paclitaxel

Abstract

The aim of this study was to prepare and characterize an innovative hepatocellular carcinoma-targeted therapeutic drug delivery system based on folate-PEG-mesoporous silica nanoparticles (FA-PEG-MSNs) loaded with paclitaxel (PTX). In vitro cell experiments and an in vivo antitumor efficacy study demonstrated that FA-PEG-MSNs-PTX produced significantly higher tumor inhibition compared with pure PTX and mesoporous silica nanoparticles loaded with paclitaxel (MSNs-PTX). The biodistribution investigation of PTX in nude mice revealed that the FA-PEG-MSNs-PTX could accumulate in tumors. Folic acid functionalized MSNs resulted in a good targeting effect, confirming that FA-PEG-MSNs-PTX is a promising tumor-targeted drug delivery system for liver cancer chemotherapy.

Quercetin loaded folate targeted plasmonic silver nanoparticles for light activated chemo-photothermal therapy of DMBA induced breast cancer in Sprague Dawley rats

“…This accentuated internalization may be a result of better recognition of FA functionalized AgNPs by FRs abundantly expressed on breast cancer cells, thus allowing adequate tumor specic accumulation of QRC-FA-AgNPs without harming the adjacent normal tissues. This observation was in accordance with previous studies involving diversied nanoscale carriers such as silver, 64 mesoporous silica, lignin based hollow nanoparticles, 70 Janus silver/silica based nanoplatforms 24 and so forth that revealed specic internalization and localization of nanoconjugates inside cellular organelles and the perinuclear region in each case. In order to conrm the FR targeted cellular uptake, co-incubation of cells with free folic acid was performed, which then indicated signicant reduction in the green uorescence of FA fabricated QRC-AgNPs, clearly suggesting that owing to competitive blockage of the FRs during FA pre-incubation resulted in diminished endocytosis of the nanoscopic-based cargoes.…”

Preparation of paclitaxel-folic acid functionalized gelatin grafted mesoporous hollow carbon nanospheres for enhancing antitumor effects toward liver cancer (SMMC-7721) cell lines

“…APTES (2 ml) was added to the system. The reaction was carried out for 12 h. 27 The product obtained by centrifugation was washed three times with water and was labeled as MCN-NH2. Folic acid (500 mg) was dissolved in 10 ml DMSO containing triethylamine and then DCC (0.25 g) and NHS (0.26 g) were added to the system overnight.…”

Mesoporous Silica Nanoparticles: Properties and Strategies for Enhancing Clinical Effect

“…Proteins mAbs Specific binding with surface antigens on target cells [190][191][192][193][194] Fabs Specific binding with surface antigens on target cells [195,196] Transferrin Binds to overexpressed transferrin receptor 1 [167,[197][198][199] Affibodies Engineered proteins designed to selectively bind to specific receptor on target cell [61,200] Heparin Anti-angiogenesis agent and ligand-receptor targeting with overexpressed surface heparanase [201] Peptides RGD Overexpressed integrin α V β 3 are selectively bound [128,166,202] pHLIPs Transmembrane insertion resulting from acidic tumor microenvironment [38,62] CPPs Specific or nonspecific interaction with the cell membrane or proteins on its surface [189,[203][204][205][206][207][208][209] Nucleic Acids Aptamers Overexpressed surface receptor proteins (e.g., GLUT1) are targeted by designed nucleic acid chains [60,[210][211][212][213][214] Small Molecules Folate/Folic Acid Ligand-receptor targeting between folate and folate receptor α [202,[215][216][217][218][219][220] Hyaluronic Acid Overexpressed CD44 on tumor cell surfaces binds with HA…”

“…It should be noted that short HA chain lengths should be used when using HA for targeting, as they increase the engulfment efficiency [ 264 ]. Tumor cell overexpression of folate receptor α encourage the use of folate as another small molecule active targeting option for MSNs [ 202 , 215 , 216 , 217 , 218 , 219 , 220 ].…”