Folate: methods of analysis

Arcot, Jayashree; Shrestha, Ashok K.

doi:10.1016/j.tifs.2005.03.013

Cited by 162 publications

(157 citation statements)

References 90 publications

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“…Despite the speed and convenience of this assay, this method has varied affinity for different forms of folate and is suitable only for foods with the same form of folate. It is less useful for analyzing foods with several types of folate forms and more complex matrices (Arcot and Shrestha 2005;LeBlanc et al 2007).…”

Section: Folate Analysis Methodsmentioning

confidence: 99%

“…In this assay, the growth of microorganisms is dependent on the amount of folate in the medium; therefore, the rate of microorganism growth is measured by change in solution turbidity, presents the amount of folate. The microbiological method has been considered to be one of the most versatile methods for determining food folate, but it has some limitations such as providing inadequate information on the forms of folate, as well as the fact that it needs experts, and it can also be a tedious procedure (Arcot and Shrestha 2005).…”

Section: Folate Analysis Methodsmentioning

confidence: 99%

“…Some of them are hard and expensive to detect, which constitutes a potential major limitation. In addition, HPLC is not able to separate folates based on their structure and size at the same time (Arcot and Shrestha 2005;LeBlanc et al 2007;Lin and Young 2000). However, HPLC has been used to measure folate in several foods including dairy products (Gregory 3rd et al 1984;Holt et al 1988).…”

Section: Folate Analysis Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Folate bio-fortification of yoghurt and fermented milk: a review

Rad

Khosroushahi

Khalili

et al. 2016

Dairy Sci. & Technol.

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Folate, an essential vitamin, plays an important role in human life for the synthesis of nucleotides, vitamins and some amino acid. This vitamin cannot be synthesized by humans and must be obtained exogenously to prevent folate deficiency, neural tube defects (NTDs) and other related diseases. Folic acid, the chemically synthesized form of folate, is used for fortification and supplementation, but it can cause adverse effects such as vitamin B 12 deficiency in high intake. Therefore, novel methods to increase concentrations of naturally occurring folate in foods have grabbed the interest of researchers. The application of bacterial cultures in food fermentation is a novel strategy to increase "natural" folate levels. Dairy products, especially yoghurt, are an appropriate choice for bio-fortification of folate as they contain folate-binding protein which improves folate bioavailability. This study will review the folate production by probiotic bacteria in fermented milk based on various strains and culture conditions.

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Section: Folate Analysis Methodsmentioning

confidence: 99%

Section: Folate Analysis Methodsmentioning

confidence: 99%

Section: Folate Analysis Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Folate bio-fortification of yoghurt and fermented milk: a review

Rad

Khosroushahi

Khalili

et al. 2016

Dairy Sci. & Technol.

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“…Folic acid is susceptible to cleavage under acidic conditions, light, and high temperature [1]. Folic acid can promote the formation of red blood cells and is identified as an anti-anemia and growth factor.…”

Section: Introductionmentioning

confidence: 99%

“…But analysis of folic acid is not easy due to its lower stability, presence in lower concentration in biological systems, and complex extraction and detection techniques. Methods of analysis for folic acid are grouped into microbiological, bio-specific procedures, chromatographic, and chemical methods [1]. Although microbiological assay is the most commonly used method, it is time consuming, needs great care and skill [4].…”

Section: Introductionmentioning

confidence: 99%

Determination of folic acid by chemiluminescence based on peroxomonosulfate-cobalt(II) system

Zhang

Zhao

Lin

2008

Talanta

119

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Based on the chemiluminescence (CL) phenomena of folic acid in peroxomonosulfate-cobalt(II) system, a rapid and sensitive CL method was developed for determination of folic acid in pharmaceutical preparations and its urinary metabolism processes. Under the optimum conditions, the relative CL intensity was linear over the concentration ranging from 10 −9 to 8 × 10 −7 mol L −1 (R 2 = 0.9991) with a detection limit as low as 6 × 10 −10 mol L −1 (S/N = 3) and relative standard deviation was 2.63% for 2 × 10 −8 mol L −1 folic acid (n = 11). This method has been successfully applied to the determination of folic acid in tablets and human urine. The blank CL emission was yielded owing to the formation of singlet oxygen molecular pair from the quenching experiment of 1,4-diazabicyclo[2.2.2]octane, and pterine-6-carboxylic acid might be the degradation intermediate in this system and it also acts an energy acceptor and sensitizes the chemiluminescence based on the studies of the CL and fluorescence spectra.

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