Foliar Application of Different Vegetal-Derived Protein Hydrolysates Distinctively Modulates Tomato Root Development and Metabolism

Ceccarelli, Angela Valentina; Miras-Moreno, Begoña; Buffagni, Valentina; Senizza, Biancamaria; Pii, Youry; Cardarelli, Mariateresa; Rouphael, Youssef; Colla, Giuseppe; Lucini, Luigi

doi:10.3390/plants10020326

Cited by 51 publications

(60 citation statements)

References 55 publications

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“…3 The effect of seed priming with aqueous duckweed extracts on a leaf area and b chlorophyll content in maize at 21 DAS. Letters, if different, indicate statistically significant differences for P < 0.05 between treatments (n = 3) line with previous studies on other plant-based biostimulants (Ceccarelli et al 2021).…”

Section: Discussionsupporting

confidence: 89%

Physiological and Biochemical Effects of an Aqueous Extract of Lemna minor L. as a Potential Biostimulant for Maize

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Bartucca

Ballerini

et al. 2021

J Plant Growth Regul

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Biostimulants are receiving increasing attention for their beneficial effects on crops, driving interest in identifying new plant extracts that could exert such stimulatory effects. This work aimed to evaluate the potential of an aqueous extract obtained from duckweed (Lemna minor L.), a freshwater species, to act as a biostimulant in maize. For this purpose, duckweed plants were collected from a natural basin and then transferred, stabilized, and grown under controlled conditions. The duckweed extract was first characterized through untargeted profiling, which revealed an abundance of bioactive phytochemicals. A relatively high amount of low-molecular-weight secondary metabolites such as phenolics (6714.99 mg kg−1) and glucosinolates (4563.74 mg kg−1) were present in the plant extract. Maize seeds were primed with different concentrations of this extract (0.01%, 0.05%, 0.50%, and 1.00%, dry weight/water volume), and some physiological and biochemical traits of the crop were recorded. The duckweed extract improved maize germination, biomass, leaf area, pigment content, and vigor index. The most effective treatment was the 0.50% concentration, which improved the majority of the measured growth traits. The extract at concentrations of 0.05%, 0.50%, and 1.00% stimulated the assimilation of nitrogen (N), phosphorous (P), potassium (K), calcium (Ca), magnesium (Mg), sodium (Na), iron (Fe), and copper (Cu). In summary, this study revealed that duckweed is a promising species that can be cultured and grown under controlled conditions for obtaining extracts with biostimulant properties.

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Section: Discussionsupporting

confidence: 89%

Physiological and Biochemical Effects of an Aqueous Extract of Lemna minor L. as a Potential Biostimulant for Maize

Buono

Bartucca

Ballerini

et al. 2021

J Plant Growth Regul

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“…Three of them were commercial products obtained by thermal-chemical hydrolysis of animal-derived proteins [Siapton ® (I) commercialized by Sumitomo Chemical Italia S.r.l., Milano, Italy] or enzymatic hydrolysis of vegetal-derived proteins [Trainer ® (D), and Vegamin ® (H) commercialized by Hello Nature Inc. (former Italpollina), Anderson, IN, United States]. The other eight PHs were obtained from vegetal proteins by enzymatic hydrolysis as described previously ( Colantoni et al, 2017 ; Ceccarelli et al, 2021 ). Plant biomass from Fabaceae (A, G, O), Malvaceae (C), Brassicaceae (F), Solanaceae (B), and Graminaceae (E, P) were used as protein sources for the other eight PHs.…”

Section: Methodsmentioning

confidence: 99%

Seed Priming With Protein Hydrolysates Improves Arabidopsis Growth and Stress Tolerance to Abiotic Stresses

et al. 2021

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The use of plant biostimulants contributes to more sustainable and environmentally friendly farming techniques and offers a sustainable alternative to mitigate the adverse effects of stress. Protein hydrolysate-based biostimulants have been described to promote plant growth and reduce the negative effect of abiotic stresses in different crops. However, limited information is available about their mechanism of action, how plants perceive their application, and which metabolic pathways are activating. Here we used a multi-trait high-throughput screening approach based on simple RGB imaging and combined with untargeted metabolomics to screen and unravel the mode of action/mechanism of protein hydrolysates in Arabidopsis plants grown in optimal and in salt-stress conditions (0, 75, or 150 mM NaCl). Eleven protein hydrolysates from different protein sources were used as priming agents in Arabidopsis seeds in three different concentrations (0.001, 0.01, or 0.1 μl ml–1). Growth and development-related traits as early seedling establishment, growth response under stress and photosynthetic performance of the plants were dynamically scored throughout and at the end of the growth period. To effectively classify the functional properties of the 11 products a Plant Biostimulant Characterization (PBC) index was used, which helped to characterize the activity of a protein hydrolysate based on its ability to promote plant growth and mitigate stress, and to categorize the products as plant growth promoters, growth inhibitors and/or stress alleviator. Out of 11 products, two were identified as highly effective growth regulators and stress alleviators because they showed a PBC index always above 0.51. Using the untargeted metabolomics approach, we showed that plants primed with these best performing biostimulants had reduced contents of stress-related molecules (such as flavonoids and terpenoids, and some degradation/conjugation compounds of phytohormones such as cytokinins, auxins, gibberellins, etc.), which alleviated the salt stress response-related growth inhibition.

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“…Other application of protein hydrolysates refer to plants subjected to stress conditions such as in the study of Koleška et al [ 136 ] who tested the effectiveness of biostimulants in alleviating macronutrient deficiency effects on tomato plants, or Casadesús et al [ 137 ] and Ertani et al [ 138 ] who studied the hormonal effects of plant biostimulants on water-stressed tomato plants. It is suggested that foliar or root applications of protein hydrolysates may improve root development, C and N assimilation and nutrients uptake from plants [ 33 , 139 , 140 ], regulate the metabolic processes through a multi-level signaling that involves auxin-like activities [ 141 , 142 , 143 ], as well as to increase the effectiveness of plant defense mechanisms against abiotic stressors in a sustainable manner [ 144 ]. However, apart from increased tolerance to stressors the application of protein hydrolysates may enhance quality parameters in fruit and leafy vegetables [ 35 , 105 , 145 ].…”

Section: Practical Applications Of Biostimulants and Biostimulatory Products On Horticultural Cropsmentioning

confidence: 99%

Biostimulants Application: A Low Input Cropping Management Tool for Sustainable Farming of Vegetables

et al. 2021

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Biostimulants, are a diverse class of compounds including substances or microorganism which have positive impacts on plant growth, yield and chemical composition as well as boosting effects to biotic and abiotic stress tolerance. The major plant biostimulants are hydrolysates of plant or animal protein and other compounds that contain nitrogen, humic substances, extracts of seaweeds, biopolymers, compounds of microbial origin, phosphite, and silicon, among others. The mechanisms involved in the protective effects of biostimulants are varied depending on the compound and/or crop and mostly related with improved physiological processes and plant morphology aspects such as the enhanced root formation and elongation, increased nutrient uptake, improvement in seed germination rates and better crop establishment, increased cation exchange, decreased leaching, detoxification of heavy metals, mechanisms involved in stomatal conductance and plant transpiration or the stimulation of plant immune systems against stressors. The aim of this review was to provide an overview of the application of plant biostimulants on different crops within the framework of sustainable crop management, aiming to gather critical information regarding their positive effects on plant growth and yield, as well as on the quality of the final product. Moreover, the main limitations of such practice as well as the future prospects of biostimulants research will be presented.

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Foliar Application of Different Vegetal-Derived Protein Hydrolysates Distinctively Modulates Tomato Root Development and Metabolism

Cited by 51 publications

References 55 publications

Physiological and Biochemical Effects of an Aqueous Extract of Lemna minor L. as a Potential Biostimulant for Maize

Physiological and Biochemical Effects of an Aqueous Extract of Lemna minor L. as a Potential Biostimulant for Maize

Seed Priming With Protein Hydrolysates Improves Arabidopsis Growth and Stress Tolerance to Abiotic Stresses

Biostimulants Application: A Low Input Cropping Management Tool for Sustainable Farming of Vegetables

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