As a result of three-year studies, we have established a growth in the yield and feed advantages of variable alfalfa in the variants with inoculation of seeds with virulent active strains of rhizobia. The total yield augmentation of green mass in the experimental variants over the three years of alfalfa life were 6.8–13.7 % compared to the control ones. The positive effect of inoculation with virulent active rhizobia strains on the total collection of dry matter was expressed in its increase in experimental conditions by 12.6–21.7 %. The highest yield of green mass, as well as dry matter was obtained in the variant with the inoculation of alfalfa seeds with the main production strain 425. The researched factor has a positive effect on the collection of feed units, digestible protein and feed protein units from 1 ha. The collection of feed units per hectare in the experimental versions increases by 1.1–1.3 times, the collection of digestible protein – by 1.2–1.4 times. The maximum substance of feed units and digestible protein per hectare was observed in the version with seed inoculation with strain 425a. The provision of a feed unit with digestible protein increases by 10.44–18.18 g or by 6.1–10.6 %.