Follicular fluid exosomes regulate OVGP1 secretion in yak oviduct epithelial cells via autophagy in vitro

Wang, Jinglei; Wang, Meng; Xu, Ruihua; Han, Xiaobin; Ma, Rui; Zhao, Ling; Zhang, Tongxiang; Wang, Yaying; Zhao, Tian; Ding, Tianyi; Baloch, Abdul Rasheed; Wang, Yunlong; Cui, Yan; Yu, Sijiu

doi:10.1002/jcp.30988

Cited by 4 publications

(3 citation statements)

References 54 publications

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“…The PI3K/AKT signaling pathway, known for its role in insulin signaling, is closely associated with adipogenesis [ 32 ]. Although previous studies on yaks have primarily focused on fibroblast proliferation [ 33 ], intestinal inflammation [ 34 ], and reproduction [ 35 ], there is limited research on fat metabolism. Numerous studies have indicated that miR-129 is involved in regulating the PI3K/AKT pathway [ 16 , 17 , 18 ].…”

Section: Discussionmentioning

confidence: 99%

miR-129 Regulates Yak Intramuscular Preadipocyte Proliferation and Differentiation through the PI3K/AKT Pathway

Qin,

Wang,

Zhong

et al. 2024

IJMS

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miR-129 plays a crucial role in regulating various cellular processes, including adipogenesis; however, its downstream molecular mechanisms remain unclear. In this study, we demonstrated that miR-129 promotes yak adipogenesis in vitro via the PI3K/AKT pathway. Overexpression and interference of miR-129 in yak intramuscular preadipocytes (YIMAs) enhanced and inhibited cell differentiation, respectively, with corresponding changes in cell proliferation. Further investigation revealed that miR-129 enhances AKT and p-AKT activity in the AKT pathway without affecting cell apoptosis, and a specific inhibitor (LY294002) was used to confirm that miR-129 regulates YIMAs proliferation and differentiation through the PI3K/AKT pathway. Our findings suggest that miR-129 promotes yak adipogenesis by enhancing PI3K/AKT pathway activity. This study provides the foundation to precisely elucidate the molecular mechanism of miR-129 in YIMAs adipogenesis and develop advanced miRNA-based strategies to improve meat nutrition and obesity-related ailments in beef production.

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Section: Discussionmentioning

confidence: 99%

miR-129 Regulates Yak Intramuscular Preadipocyte Proliferation and Differentiation through the PI3K/AKT Pathway

Qin,

Wang,

Zhong

et al. 2024

IJMS

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“…So far, the cells related to the yak that have been isolated and cultured include yak germ cells, precursor adipocytes, rumen epithelial cells, fetal fibroblasts, mammary epithelial cells, etc. [ 9 , 10 , 11 , 12 ]. It is of great significance to construct an important yak population cell line to conserve its genetic resources from the perspective of our germplasm resource conservation.…”

Section: Introductionmentioning

confidence: 99%

Establishment of SV40 Large T-Antigen-Immortalized Yak Rumen Fibroblast Cell Line and the Fibroblast Responses to Lipopolysaccharide

Wang,

Yue,

Che

et al. 2023

Toxins

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The yak lives in harsh alpine environments and the rumen plays a crucial role in the digestive system. Rumen-associated cells have unique adaptations and functions. The yak rumen fibroblast cell line (SV40T-YFB) was immortalized by introducing simian virus 40 large T antigen (SV40T) by lentivirus-mediated transfection. Further, we have reported the effects of lipopolysaccharide (LPS) of different concentrations on cell proliferation, extracellular matrix (ECM), and proinflammatory mediators in SV40T-YFB. The results showed that the immortalized yak rumen fibroblast cell lines were identified as fibroblasts that presented oval nuclei, a fusiform shape, and positive vimentin and SV40T staining after stable passage. Chromosome karyotype analysis showed diploid characteristics of yak (n = 60). LPS at different concentrations inhibited cell viability in a dose-dependent manner. SV40T-YFB treated with LPS increased mRNA expression levels of matrix metalloproteinases (MMP-2 and MMP-9), inflammatory cytokines (TNF-α, IL-1β, IL-6), and urokinase-type plasminogen activator system components (uPA, uPAR). LPS inhibits the expression of tissue inhibitors of metalloproteinases (TIMP-1 and TIMP-2), plasminogen activator inhibitor-2 (PAI-2), fibronectin (FN), anti-inflammatory factor IL-10, and collagen I (COL I) in SV40T-YFB. Overall, these results suggest that LPS inhibits cell proliferation and induces ECM degradation and inflammatory response in SV40T-YFB.

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“…Its fertility could hardly meet the demands of herdsmen and restrict the increase in yak population, as a result of relatively late sexual maturity and short estrous period of 1-2 days even in lush pasture. At present, there have been studies on the uterus (Abudureyimu et al, 2018), oviduct (Wang et al, 2023), lung (Ge et al, 2021), and heart (He et al, 2016) of yaks, but in the transition between luteal phase and follicular phase of yaks, important regulatory proteins in the cycle transition still need to be explored.…”

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confidence: 99%

Label‐free proteomic analysis reveals an estrous cycle transition between the follicular phase and the luteal phase in yak ovary

Chen

Cui

2023

Animal Science Journal

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In order to further study the physiological mechanism about yak reproduction for the improvement of its fertility, differentially expressed proteins (DEGs) of the ovary were screened during the follicular and luteal phases of yak based on label‐free quantitative proteomics. The main research results were as follows: it discovered 2867 proteins during the follicular phase and 2180 proteins during the luteal phase, among which there were 2011 proteins of expression during both phases, 856 during the follicular phase, and 169 during the luteal phase. According to the bioinformatics and Gene Ontology analysis, the screened differentially expressed proteins were mostly located in the cell membrane and extracellular region and primarily acted on the activity of laminin. Kyoto Encyclopedia of Genes and Genomes analysis indicated that the main involvement of differentially expressed proteins included steroid biosynthesis, chemokine signaling pathway, protein processing in the endoplasmic reticulum, glycerol phospholipid metabolism, carbon metabolism, PI3K‐Akt signaling pathways, PPAR signaling pathways, nucleotide oligomerization domain (NOD) receptor signaling pathways, and purine metabolic pathways. Screened proteins related to reproductive function, so as to lay a theoretical foundation for the in‐depth study of the regulation mechanism of yak reproduction and improve its fertility.

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Follicular fluid exosomes regulate OVGP1 secretion in yak oviduct epithelial cells via autophagy in vitro

Cited by 4 publications

References 54 publications

miR-129 Regulates Yak Intramuscular Preadipocyte Proliferation and Differentiation through the PI3K/AKT Pathway

miR-129 Regulates Yak Intramuscular Preadipocyte Proliferation and Differentiation through the PI3K/AKT Pathway

Establishment of SV40 Large T-Antigen-Immortalized Yak Rumen Fibroblast Cell Line and the Fibroblast Responses to Lipopolysaccharide

Label‐free proteomic analysis reveals an estrous cycle transition between the follicular phase and the luteal phase in yak ovary

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