Follicular fluid hydrogen peroxide and lipid hydroperoxide in bovine antral follicles of various size, atresia, and dominance status

Hennet, Margo L.; Yu, Hope Y.; Combelles, Catherine M.H.

doi:10.1007/s10815-012-9925-5

Cited by 18 publications

(8 citation statements)

References 39 publications

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“…In combination with experiments using a NOX4 blocker they revealed a 36% contribution of NOX4 to overall H 2 O 2 generation. Results are in line with a report on the presence of H 2 O 2 in FF 39 .…”

Section: Discussionsupporting

confidence: 92%

The NADPH oxidase 4 is a major source of hydrogen peroxide in human granulosa-lutein and granulosa tumor cells

Buck

Hack

Berg

et al. 2019

Sci Rep

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H 2 O 2 is a reactive oxygen species (ROS), which can diffuse away from its site of generation and may act as a cell-to-cell signaling factor. The mechanisms responsible for the generation of H 2 O 2 in human ovarian follicles and possible signaling role(s) of H 2 O 2 are not well known. We identified a source of H 2 O 2 , the enzyme NADPH oxidase (NOX) 4, in isolated differentiated, in-vitro fertilisation-derived human granulosa-lutein cells (GCs), in proliferating human granulosa tumour cells (KGN), as well as in situ in cells of growing ovarian follicles. H 2 O 2 was readily detected in the supernatant of cultured GCs and KGN cells. H 2 O 2 levels were significantly lowered by the NOX4 blocker GKT137831, indicating a pronounced contribution of NOX4 to overall H 2 O 2 generation by these cells. We provide evidence that extracellular H 2 O 2 is taken up by GCs, which is facilitated by aquaporins (peroxiporins). We thus conclude that GC-derived H 2 O 2 might act as autocrine/paracrine factor. Addition of H 2 O 2 increased MAPK-phosphorylation in GCs. Moreover, reducing H 2 O 2 production with GKT137831 slowed proliferation of KGN cells. Our results pinpoint NOX4 and H 2 O 2 as physiological players in the regulation of GC functions.

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Section: Discussionsupporting

confidence: 92%

The NADPH oxidase 4 is a major source of hydrogen peroxide in human granulosa-lutein and granulosa tumor cells

Buck

Hack

Berg

et al. 2019

Sci Rep

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“…In this study, we found the increased expression of PRDX4 in mature follicles compared to immature follicles (Figure 3) which was consistent with our previous study [17]. Related to ovulation, ROS concentration increased in large follicles compared to small ones [43], which may explain the increase of PRDX4 with follicular development. When the immature PCOS follicles were cultured in vitro , ROS in the environment stimulated PRDX4 expression to protect GCs from oxidative stress injury.…”

Section: Discussionsupporting

confidence: 92%

Downregulated Expression of Peroxiredoxin 4 in Granulosa Cells from Polycystic Ovary Syndrome

Meng

Qian

et al. 2013

PLoS ONE

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Peroxiredoxin 4 (PRDX4), a member of Peroxiredoxin (PRDX) family, is a typical 2-Cys PRDX. PRDX4 monitors the oxidative burden within cellular compartment and reduces hydrogen peroxide and alkyl hydroperoxide related to oxidative stress and apoptosis. Antioxidant, like PRDX4, may promote follicle development and participate in the pathophysiology of PCOS. In our previous study, we found that PRDX4 was expressed in mice oocyte cumulus oophorus complex, and that PRDX4 could be associated with follicle development. In this study, we explored the expression of PRDX4 in human follicles and possible role of PRDX4 in PCOS pathophysiology. Our data showed that PRDX4 was mainly expressed in granulosa cells in human ovaries. When compared to control group, both PRDX4 mRNA level and protein level decreased in PCOS group. The lowered levels of PRDX4 may relate to oxidative stress in the pathophysiologic progress of PCOS. Furthermore, expression of PRDX4 in the granulosa cells of in vivo or in vitro matured follicles was higher than that in immatured follicles, which suggested that PRDX4 may have a close relationship with follicular development. Altogether, our findings may provide new clues of the pathophysiologic mechanism of PCOS and potential therapeutic strategy using antioxidant, like PRDX4.

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“…Thus, a delicate equilibrium between ROS and antioxidant in the follicle microenvironment is required to counter the potentially harmful effects of ROS and to maintain the cellular functions. Follicular fluid milieu of dominant follicles is characterized by elevated concentrations of H 2 O 2, a component of ROS system [65]. Previous studies show that ovulation rates and ovulatory events are perturbed upon ROS reduction in the mouse and rabbit [66,67].…”

Section: Discussionmentioning

confidence: 99%

Quantitative proteomic profiling of bovine follicular fluid during follicle development†

Ferrazza

García

Schmidt

et al. 2017

Biology of Reproduction

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Bovine follicular fluid (FF) constitutes the microenvironment of follicles and includes various biologically active proteins. We performed a study involving 18 healthy nonlactating Holstein cows to determine the protein expression profile of FF at key stages of follicular development. Follicles were individually aspirated in vivo at predeviation (F1 ∼ 7.0 mm), deviation (F1 ∼ 8.5 mm), postdeviation (F1 ∼ 12.0 mm), and preovulatory stages of follicle development, which were confirmed by measurement of follicular estradiol and progesterone concentrations. The FFs from nine cows were selected for proteomic analysis. After albumin depletion, triplicates of pooled FF were reduced, alkylated, and digested with trypsin. The resulting peptides were labeled with TMTsixplex and quantified using liquid chromatography-mass spectrometry/mass spectrometry. A total of 143 proteins were identified and assigned to a variety of biological processes, including response to stimulus and metabolic processes. Twenty-two differentially (P < 0.05) expressed proteins were found between stages indicating intrafollicular changes over development, with expected deviation time critical to modulate the protein expression. For instance, high concentrations of follistatin, inhibin, serglycin, spondin-1, fibrinogen, and anti-testosterone antibody were found during early stages of follicular development. In contrast, apolipoprotein H, alpha-2-macroglobulin, plasminogen, antithrombin-III, and immunoglobulins were increased after deviation. Among the differentially abundant proteins, 19 were found to be associated with steroidogenesis. Pathway analysis identified proteins that were mainly associated with the acute phase response signaling, coagulation system, complement system, liver/retinoid X receptor activation, and biosynthesis of nitric oxide and reactive oxygen. The differentially expressed proteins provide insights into the size-dependent protein changes in the ovarian follicle microenvironment that could influence follicular function.

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Follicular fluid hydrogen peroxide and lipid hydroperoxide in bovine antral follicles of various size, atresia, and dominance status

Cited by 18 publications

References 39 publications

The NADPH oxidase 4 is a major source of hydrogen peroxide in human granulosa-lutein and granulosa tumor cells

The NADPH oxidase 4 is a major source of hydrogen peroxide in human granulosa-lutein and granulosa tumor cells

Downregulated Expression of Peroxiredoxin 4 in Granulosa Cells from Polycystic Ovary Syndrome

Quantitative proteomic profiling of bovine follicular fluid during follicle development†

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