Force Measurement Tools to Explore Cadherin Mechanotransduction

Stapleton, Sarah C.; Chopra, Anant; Chen, Christopher

doi:10.3109/15419061.2014.905929

Cited by 15 publications

(11 citation statements)

References 90 publications

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“…E-Cad-based adhesions can alter the physical aspects of cells such as the stiffness4142, spreading43 and migration44 of single cells, and facilitate the propagation of force in the tissue4546. The NSC23766-induced or RhoA-Q63L-induced appearance of E-cad in IDE cells in gerbils likely propagates the intracellular force across intercellular contacts in tissues.…”

Section: Discussionmentioning

confidence: 99%

Fine tuning of Rac1 and RhoA alters cuspal shapes by remolding the cellular geometry

Liu

Tang

Nakamura

et al. 2016

Sci Rep

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The anatomic and functional combinations of cusps and lophs (ridges) define the tooth shape of rodent molars, which distinguishes species. The species-specific cusp patterns result from the spatiotemporal induction of enamel knots (EKs), which require precisely controlled cellular behavior to control the epithelial invagination. Despite the well-defined roles of EK in cusp patterning, the determinants of the ultimate cuspal shapes and involvement of epithelial cellular geometry are unknown. Using two typical tooth patterns, the lophodont in gerbils and the bunodont in mice, we showed that the cuspal shape is determined by the dental epithelium at the cap stage, whereas the cellular geometry in the inner dental epithelium (IDE) is correlated with the cuspal shape. Intriguingly, fine tuning Rac1 and RhoA interconvert cuspal shapes between two species by remolding the cellular geometry. Either inhibition of Rac1 or ectopic expression of RhoA could region-distinctively change the columnar shape of IDE cells in gerbils to drive invagination to produce cusps. Conversely, RhoA reduction in mice inhibited invagination and developed lophs. Furthermore, we found that Rac1 and RhoA modulate the choices of cuspal shape by coordinating adhesion junctions, actin distribution, and fibronectin localization to drive IDE invagination.

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Section: Discussionmentioning

confidence: 99%

Fine tuning of Rac1 and RhoA alters cuspal shapes by remolding the cellular geometry

Liu

Tang

Nakamura

et al. 2016

Sci Rep

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“…In particular, precisely combining two or more ligands within an engineered environment has already helped to unravel the combinatorial interplay of different adhesive pathways. For example, an increasing number of studies scrutinize the crosstalk between integrins and growth factors (Fourel et al, 2016;Lee et al, 2011), as well as between integrins and cadherins (Borghi et al, 2010;Stapleton et al, 2014), by incorporating one factor at a time in order to generate in vitro the essential aspects of in vivo complexity. Because the downstream consequences of environmental sensing most likely originate from the integrated signaling of combined environmental cues, it is important to reconstruct the complexity of the microniche from individually controlled cues.…”

Section: Discussionmentioning

confidence: 99%

How cells respond to environmental cues – insights from bio-functionalized substrates

Ruprecht

Monzo

Ravasio

et al. 2016

Journal of Cell Science

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Biomimetic materials have long been the (he)art of bioengineering. They usually aim at mimicking in vivo conditions to allow in vitro culture, differentiation and expansion of cells. The past decade has witnessed a considerable amount of progress in soft lithography, bioinspired micro-fabrication and biochemistry, allowing the design of sophisticated and physiologically relevant micro-and nanoenvironments. These systems now provide an exquisite toolbox with which we can control a large set of physicochemical environmental parameters that determine cell behavior. Biofunctionalized surfaces have evolved from simple protein-coated solid surfaces or cellular extracts into nano-textured 3D surfaces with controlled rheological and topographical properties. The mechanobiological molecular processes by which cells interact and sense their environment can now be unambiguously understood down to the single-molecule level. This Commentary highlights recent successful examples where bio-functionalized substrates have contributed in raising and answering new questions in the area of extracellular matrix sensing by cells, cell-cell adhesion and cell migration. The use, the availability, the impact and the challenges of such approaches in the field of biology are discussed.

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“…Traction force measurements have become a widely used methodology, but measurements are largely limited to forces applied to the cell-substrate interface. 26,27,[36][37][38] The NMBS can be applied to the apical surface of the cell to provide new information on membrane deformation. To demonstrate this, the NMBS was applied to the apical surface of human skeletal muscle cells (HSMC) grown in culture using the 2 µm x 2 µm mesh to provide subcellular resolution.…”

Section: Biosensormentioning

confidence: 99%

Fibronectin-Based Nanomechanical Biosensors to Map 3D Strains in Live Cells and Tissues

Shiwarski

Tashman

Tsamis

et al. 2020

Preprint

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Mechanical forces are integral to a wide range of cellular processes including migration, differentiation and tissue morphogenesis; however, it has proved challenging to directly measure strain at high spatial resolution and with minimal tissue perturbation. Here, we fabricated, calibrated, and tested a fibronectin (FN)-based nanomechanical biosensor (NMBS) that can be applied to cells and tissues to measure the magnitude, direction, and dynamics of strain from subcellular to tissue length-scales. The NMBS is a fluorescently-labeled, ultrathin square lattice FN mesh with spatial resolution tailored by adjusting the width and spacing of the lattice fibers from 2-100 µm. Time-lapse 3D confocal imaging of the NMBS demonstrated strain tracking in 2D and 3D following mechanical deformation of known materials and was validated with finite element modeling. Imaging and 3D analysis of the NMBS applied to single cells, cell monolayers, and Drosophila ovarioles demonstrated the ability to dynamically track microscopic tensile and compressive strains in various biological applications with minimal tissue perturbation. This fabrication and analysis platform serves as a novel tool for studying cells, tissues, and more complex systems where forces guide structure and function.

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Force Measurement Tools to Explore Cadherin Mechanotransduction

Cited by 15 publications

References 90 publications

Fine tuning of Rac1 and RhoA alters cuspal shapes by remolding the cellular geometry

Fine tuning of Rac1 and RhoA alters cuspal shapes by remolding the cellular geometry

How cells respond to environmental cues – insights from bio-functionalized substrates

Fibronectin-Based Nanomechanical Biosensors to Map 3D Strains in Live Cells and Tissues

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