“…Currently, some Y-STR-specific kits are being improved to provide complete profiles, namely the Yfiler â Plus PCR Amplification Kit (Applied Biosystems, Waltham, MA) (9,11) and the PowerPlex â Y23 System (Promega â , Madison, WI), hereafter called Yfiler â Plus and PowerPlex Y23 â , respectively. The Yfiler â Plus kit simultaneously targets 27 markers on the Y chromosome (e.g., 25 markers, of which two of are referred to as heterozygote markers), and PowerPlex Y-23 â targets 23 markers on the Y chromosome (one of which is a heterozygote marker).…”