Forensic DNA typing by capillary electrophoresis using the ABI Prism 310 and 3100 genetic analyzers for STR analysis

Butler, John M.; Buel, Eric; Crivellente, Federica; McCord, Bruce

doi:10.1002/elps.200305822

Cited by 215 publications

(141 citation statements)

References 63 publications

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“…3,7,8 The use of fluorescence-based approaches allows performing large-scale human genetic tests and significantly reduces contamination because of keeping laboratory microtubes closed after PCR amplification. Accordingly, we checked the possibility of polymorphic Alu allelic discrimination by means of real-time PCR with Sybr Green dye and dissociation curves analysis using two primer pairs from our set.…”

Section: Resultsmentioning

confidence: 99%

“…Many methodological approaches are used at the second stage, most of them involving precise determination of PCR products' length by PAGE or capillary electrophoresis with fluorescent labeling of analyzed DNA fragments, as well as quantitative real-time PCR, MALDI or microarray technology. [8][9][10] Although the latter methods are usually automated and have become a routine procedure, they still remain expensive because of the use of special facilities and fluorescent dyes. In the past decade, progress in human genome sequencing and methods of comparative genomics resulted in the identification of a new type of MGMs based on retroelement (RE) insertion polymorphism.…”

Section: Introductionmentioning

confidence: 99%

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A new set of markers for human identification based on 32 polymorphic Alu insertions

Mamedov

Shagina²,

Kurnikova³

et al. 2010

Eur J Hum Genet

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A number of genetic systems for human genetic identification based on short tandem repeats or single nucleotide polymorphisms are widely used for crime detection, kinship studies and in analysis of victims of mass disasters. Here, we have developed a new set of 32 molecular genetic markers for human genetic identification based on polymorphic retroelement insertions. Allele frequencies were determined in a group of 90 unrelated individuals from four genetically distant populations of the Russian Federation. The mean match probability and probability of paternal exclusion, calculated based on population data, were 5.53Â10 À14 and 99.784%, respectively. The developed system is cheap and easy to use as compared to all previously published methods. The application of fluorescence-based methods for allele discrimination allows to use the human genetic identification set in automatic and high-throughput formats.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A new set of markers for human identification based on 32 polymorphic Alu insertions

Mamedov

Shagina²,

Kurnikova³

et al. 2010

Eur J Hum Genet

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show abstract

“…Butler et al [89] have recently reported on sample preparation, injection, separation, detection, and interpretation of STR results using the widely used ABI prism 310 and 3100 genetic analyzers [89].…”

Section: Forensic Dna and Rna Analysismentioning

confidence: 99%

Recent advances in the applications of CE to forensic sciences (2001–2004)

Tagliaro

Bortolotti

2006

Electrophoresis

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Recent advances in the applications of CE to forensic sciences (2001-2004)The present article reviews the applications of CE in forensic science covering the period from 2001 until the first part of 2005. The overview includes the most relevant examples of analytical applications of capillary electrophoretic and electrokinetic techniques in the following fields: (i) Forensic drugs and poisons, (ii) explosive analysis and gunshot residues, (iii) small ions of forensic interest, (iv) forensic DNA and RNA analysis, (v) proteins of forensic interest, and (vi) ink analysis.

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“…Additionally, electrophoresis-based methods only differentiate genotypes of length polymorphisms, while nucleotide variations such as single nucleotide polymorphisms (SNPs) in the flanking sequences of a repeat motif cannot be detected. Therefore, more potentials of the marker system have been restrained by the approaches to analyze the markers (Butler et al, 2004;Butler, 2007;Pascali et al, 2012). Thus, there is an urgent for new STR genotyping approaches.…”

Section: Introductionmentioning

confidence: 99%

Y-STR genetic screening by high-resolution melting analysis

et al. 2016

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ABSTRACT. Currently, the widely used automated capillary electrophoresis-based short tandem repeat (STR) genotyping method for genetic screening in forensic practice is laborious, time-consuming, expensive, and technically challenging in some cases. Thus, new molecular-based strategies for conclusively identifying forensically relevant biological evidence are required. Here, we used high-resolution melting analysis (HRM) for Y-chromosome STR genotyping for forensic genetic screening. The reproducibility of the melting profile over dilution, sensitivity, discrimination power, and other factors was preliminarily studied in 10 Y-STR loci. The results showed that HRM-based approaches revealed more genotypes (compared to capillary electrophoresis), showed higher uniformity in replicate tests and diluted samples, and enabled successful detection of DNA at concentrations as low as 0.25 ng. For mixed samples, the melting curve profiles discriminated between mixed samples based on reference samples with high efficiency. The triplex Y-chromosome STR HRM assay was performed and provided a foundation for further studies such as a multiplex HRM assay. The HRM approach is a one-step application and the entire procedure can be completed within 2 h at a low cost. In conclusion, our findings demonstrate that the HRM-based Y-STR assay is a useful screening tool that can be used in forensic practice.

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Forensic DNA typing by capillary electrophoresis using the ABI Prism 310 and 3100 genetic analyzers for STR analysis

Cited by 215 publications

References 63 publications

A new set of markers for human identification based on 32 polymorphic Alu insertions

A new set of markers for human identification based on 32 polymorphic Alu insertions

Recent advances in the applications of CE to forensic sciences (2001–2004)

Y-STR genetic screening by high-resolution melting analysis

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