Forensic genetic case study:  Species identification and traceability of sea turtle caught in illegal trade in Bali, Indonesia

Pertiwi, Ni Putu Dian; Suhendro, Maulid Dio; Yusmalinda, Ni Luh Astria; Putra, I Nyoman Giri; Putri, I Gusti Ricca Mahatma; Artiningsih, Enex Yuni; Malik, Muhammad Danie Al; Cahyani, Ni Kadek Dita; Sembiring, Andrianus

doi:10.13057/biodiv/d210945

Cited by 6 publications

(5 citation statements)

References 45 publications

(66 reference statements)

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“…Deer antlers, elephant ivory, shark fins, rhino horns, sea turtles, and cheetahs (Cardeñosa et al., 2017; Ewart et al., 2018; Jiang et al., 2018; Kitpipit et al., 2016; Magliolo et al., 2021; Pertiwi et al., 2020) are some animals and animal products illegally exploited in the literature reviewed. Samples for molecular analysis in wildlife preservation testing differ slightly from the authentication of game meat products.…”

Section: Dna‐based Methods For Wildlife Conservationmentioning

confidence: 99%

A systematic review of DNA‐based methods in authentication of game and less common meat species

Adenuga

Montowska

2023

Comp Rev Food Sci Food Safe

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Despite the numerous studies on food safety and authenticity, especially for meat and meat products, not enough studies have been conducted focusing exclusively on game species and other unusual meat animals. As a result of the European horse scandal, the horse is currently the target of many meat authenticity studies. With this review, we aim to present various DNA-based methods that have been used by researchers to identify, detect, and quantify game, uncommon meat animals, and wildlife species. Polymerase chain reaction (PCR) is considered the standard method for DNA analysis in meat authenticity testing. However, in this paper, we present several other methods that may or may not involve the PCR technique. For this purpose, we systematically reviewed 131 articles selected according to various criteria such as target animal species, method of analysis, year of publication, and so forth. The result of our study shows the most studied game and uncommon meat species, PCR-and non-PCR-based methods for game meat analysis, and DNA-based methods in wildlife conservation. With this study, researchers can find detailed information about frequent game species used as adulterants for regular meat products and the DNA-based techniques to identify them.

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Section: Dna‐based Methods For Wildlife Conservationmentioning

confidence: 99%

A systematic review of DNA‐based methods in authentication of game and less common meat species

Adenuga

Montowska

2023

Comp Rev Food Sci Food Safe

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“…The sequence was then aligned using MEGA X (Kumar et al, 2018). The species identity was determined by comparing sequences to GenBank database (www.ncbi.nlm.nih.gov) enforcing a homology threshold of >99 % (Malik et al, 2020;Pertiwi et al, 2020). Identification using phylogenetic tree was also reconstructed using Bayesian method with BEAST2 package program (Drummond et al, 2007;Bilderbeek and Etienne, 2018).…”

Section: Methodsmentioning

confidence: 99%

Characterization of Some Commercially Important Octopus (Mollusca: Cephalopoda) from Indonesian Waters using Mitochondrial DNA Cytochrome Oxidase Sub-Unit I (Mt-DNA COI)

2021

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As per the FAO data, octopus identification is very limited in the species level at world fishery and also they are cryptic nature. On the other hand, Indonesia is one of the top ten highest octopus exporters. This study therefore aimed to determine the species of octopus based on phylogenetic analysis of mt-DNA COI. Octopuses were collected from nine different locations throughout Indonesia, i.e., Anambas, Bangka-Belitung, Cirebon, Karimunjawa, Tuban, Lombok, Buton, Wakatobi and Jayapura. Samples were mostly in the form of tentacles that were directly collected from fishermen. After being preserved in 96% ethanol, the sample was extracted in 10% chelexÒ, PCR amplification using Folmer’s primer then was further analysed by sequencing in Sanger methods. Of the 24 samples sequenced, the results recognized four species Octopodidae belongs to the three genera, named Amphioctopus aegina, Hapalochlaena fasciata, Octopus laqueus and Octopus cyanea. Mean pair-wise distances of within-species were ranged from 0 to 5.5 % and between-species was ranged from 12.9 to 15.8 %. This study distinctly confirmed the difference between genus Amphioctopus and Hapalochlaena (15.5 %), as also between O. laqueus and O. cyanea (12.9%) which was previously not completely distinguished. Although performing species identification using DNA sequences for shallow-water benthic octopus species is perhaps considered premature, this study indicated the possible application of COI sequences for species identification, thereby providing a preliminary dataset for future DNA barcoding of octopus, in particular for Indonesia waters.

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“…Berbagai pendekatan molekuler dapat digunakan, misalnya dalam kasus forensik satwa liar (Dalton et al, 2020), identifikasi spesies produk perburuan ilegal (Ashrifurrahman et al, 2022;Pertiwi et al, 2020), atau pendekatan kasus identifikasi spesies (Knecht, 2012).…”

Section: Pendahuluanunclassified

Review: Aplikasi Histopatologi Untuk Praktik Forensik

Faulana,

Empra,

Rahmadian

et al. 2024

Borneo J Med Lab Tech

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Forensics plays an important role in investigating crimes because it can reveal everything related to a murder case, including the time and cause of the murder. Histopathology is the study of damage to body tissues and organs which can be used to help investigate crimes. The aim of this literature review is to study the role of histopathology applications for forensic practice. The method used is searching journals and books using Google Scholar, ScienceDirect, NCBI, and PubMed with the keywords histopathology, forensics, diagnosis, death, microscopy, disease, staining, and autopsy. The histology approach helps the pathologist make a clear determination on the cause of death. The aim of histopathology is to perfect the macroscopic diagnosis and act as an additional examination in forensic case management. One of the histopathology methods in the forensic field is the paraffin method with hematoxylin eosin staining and special stains, immunohistochemistry, and MITS. The parameters observed with this method are measurements of pulmonary fat embolism; determining the age of myocardial infarction; assessment of liver steatosis, fibrosis, and cirrhosis; determination of the age of thrombus or thromboembolism; and diagnosis of shock with various diseases. The prospect of forensic histopathology is to complement molecular diagnostic methods, so that the combination of molecular and histology methods can play a maximum role in the field of forensic pathology. The conclusion of this literature review is that histopathology can be used to confirm the diagnosis in forensic cases and has good future prospects if combined with other methods such as molecular.

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Forensic genetic case study: Species identification and traceability of sea turtle caught in illegal trade in Bali, Indonesia

Cited by 6 publications

References 45 publications

A systematic review of DNA‐based methods in authentication of game and less common meat species

A systematic review of DNA‐based methods in authentication of game and less common meat species

Characterization of Some Commercially Important Octopus (Mollusca: Cephalopoda) from Indonesian Waters using Mitochondrial DNA Cytochrome Oxidase Sub-Unit I (Mt-DNA COI)

Review: Aplikasi Histopatologi Untuk Praktik Forensik

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