Forensic Science

Brettell, Thomas A.; Inman, Keith; Rudin, Norah; Saferstein, Richard

doi:10.1021/a1990009c

Cited by 12 publications

(1 citation statement)

References 699 publications

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“…As most kinases are capable of phosphorylating multiple proteins, it is likely that the majority of the plant proteome has the potential to be phosphorylated, if only transiently. Technology and methodology to experimentally catalog the plant phosphoproteome have existed for over a decade (Neubauer and Mann, 1999; Ficarro et al, 2002; Gruhler et al, 2005), and there has been a steady increase in the number of mapped phosphorylation sites collectively from model and crop plants (Nuhse et al, 2004, 2007; Wolschin and Weckwerth, 2005; Benschop et al, 2007; de la Fuente van Bentem et al, 2008; Sugiyama et al, 2008; Whiteman et al, 2008; Hsu et al, 2009; Ito et al, 2009; Jones et al, 2009; Li et al, 2009; Reiland et al, 2009; Wang et al, 2009; Chen et al, 2010; Grimsrud et al, 2010; Kline et al, 2010; Nakagami et al, 2010; Bi et al, 2011; Engelsberger and Schulze, 2012; Meyer et al, 2012). …”

Section: Introductionmentioning

confidence: 99%

P3DB: An Integrated Database for Plant Protein Phosphorylation

Yao¹,

Bollinger²,

Gao³

et al. 2012

Front. Plant Sci.

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Protein phosphorylation is widely recognized as the most widespread, enzyme-catalyzed post-translational modification in eukaryotes. In particular, plants have appropriated this signaling mechanism as evidenced by the twofold higher frequency of protein kinases within the genome compared to other eukaryotes. While all aspects of plant protein phosphorylation research have grown in the past 10 years; phosphorylation site mapping using high-resolution mass spectrometry has grown exponentially. In Arabidopsis alone there are thousands of experimentally determined phosphorylation sites. To archive these events in a user-intuitive format we have developed P3DB, the Plant Protein Phosphorylation Database (). This database is a repository for plant protein phosphorylation site data, currently hosting information on 32,963 non-redundant sites collated from 23 experimental studies from six plant species. These data can be queried for a protein-of-interest using an integrated BLAST module to query similar sequences with known phosphorylation sites among the multiple plants currently investigated. The paper demonstrates how this resource can help identify functionally conserved phosphorylation sites in plants using a multi-system approach.

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Section: Introductionmentioning

confidence: 99%

P3DB: An Integrated Database for Plant Protein Phosphorylation

Yao¹,

Bollinger²,

Gao³

et al. 2012

Front. Plant Sci.

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On-chip chiral and achiral separation of amphetamine and related compounds labeled with 4-fluoro-7-nitrobenzofurazane

et al. 2000

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Amphetamine and analogous compounds have been labeled with 4-fluoro-7-nitrobenzofurazane and analyzed on a microfabricated chip. Separation of norephedrine, ephedrine, cathinone, pseudoephedrine, methcathinone, amphetamine and methamphetamine is demonstrated using micellar electrokinetic capillary chromatography (MEKC) and laser-induced fluorescence (LIF) detection. Chiral separations of individual drugs were studied using neutral and negatively charged cyclodextrins (CDs) with and without the addition of an organic modifier and/or sodium dodecyl sulfate (SDS). The best results were obtained using a highly sulfated gamma-CD (HS-gamm-CD) in combination with a low concentration of SDS. To obtain complete separation of a mixture of (+/-)-norephedrine, (+/-)ephedrine, (+/-)-pseudoephedrine, (+/-)-methcathinone, (+/-)-amphetamine and (+/-)-methamphetamine it was necessary to add a small amount (1.5 mM) of SDS to the separation buffer. Optimized chiral separation was achieved within 7 min using an S-folded separation channel, a separation voltage of 8 kV and a buffer consisting of 50 mM phosphate (pH 7.35), 10 mM HS-gamma-CD and 1.5 mM SDS.

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Capillary Electrophoresis-Mass Spectrometry of Biologically Active Peptides and Proteins

Naylor¹,

Tomlinson²

2001

Clinical and Forensic Applications of Capillary Electrophoresis

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Forensic Science

Cited by 12 publications

References 699 publications

P3DB: An Integrated Database for Plant Protein Phosphorylation

P3DB: An Integrated Database for Plant Protein Phosphorylation

On-chip chiral and achiral separation of amphetamine and related compounds labeled with 4-fluoro-7-nitrobenzofurazane

Capillary Electrophoresis-Mass Spectrometry of Biologically Active Peptides and Proteins

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