Form-Deprivation Myopia in Chick Induces Limited Changes in Retinal Gene Expression

McGlinn, Alice M.; Baldwin, Donald; Tobias, John W.; Budak, Murat T.; Khurana, Tejvir S.; Stone, Richard A.

doi:10.1167/iovs.06-1538

Cited by 62 publications

(103 citation statements)

References 66 publications

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“…For example, the combined tissue collection of the RPE and retina in early gene expression studies involving the chick precludes conclusion about the site of observed changes in expression. 83,84 Nonetheless, the report of down-regulation of Bone Morphogenetic Protein (BMP2) gene expression in retina/RPE after form deprivation of chicks for either 6 h or 3 days is consistent with findings in isolated chick RPE with prolonged negative lens treatment. 85 These studies made use of high-throughput gene expression profiling with DNA microarray screening.…”

Section: The Role Of the Rpe In Eye Growth Regulationsupporting

confidence: 75%

RPE and Choroid Mechanisms Underlying Ocular Growth and Myopia

Zhang¹,

Wildsoet²

2015

Progress in Molecular Biology and Translational Science

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Myopia is the most common type of refractive errors and one of the world’s leading causes of blindness. Visual manipulations in animal models have provided convincing evidence for the role of environmental factors in myopia development. These models along with in vitro studies have provided important insights into underlying mechanisms. The key locations of the retinal pigment epithelium (RPE) and choroid make them plausible conduits for relaying growth regulatory signals originating in the retina to the sclera, which ultimately determines eye size and shape. Identifying the key signal molecules and their targets may lead to the development of new myopia control treatments. This section summarizes findings implicating the RPE and choroid in myopia development. For RPE and/or choroid, changes in morphology, activity of ion channels/transporters, as well as in gene and protein expression, have been linked to altered eye growth. Both tissues thus represent potential targets for novel therapies for myopia.

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Section: The Role Of the Rpe In Eye Growth Regulationsupporting

confidence: 75%

RPE and Choroid Mechanisms Underlying Ocular Growth and Myopia

Zhang¹,

Wildsoet²

2015

Progress in Molecular Biology and Translational Science

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“…2a, b). The previously reported myopia-related genes (Ashby et al, 2014, Hawthorne and Young, 2013, Ma et al, 2014, Mathis et al, 2014, McGlinn et al, 2007, Ritchey et al, 2012, Wisard et al, 2011, Yoshikawa et al, 2014) such as Bmp2, Ednrb, Fgf2, Igf1, Il18, Irbp, Lumican, Sfrp1, Tgfb1, Vegfa, Vip, and Wnt2b were not found in the PC1 group in vivo , which indicates that they responded less to violet light. In the PC1 group, only one myopia protective gene (Pardue et al, 2013), EGR1 (ZENK, zif268), was found among the previously reported myopia related genes (Fig.…”

Section: Resultsmentioning

confidence: 74%

Violet Light Exposure Can Be a Preventive Strategy Against Myopia Progression

et al. 2017

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Prevalence of myopia is increasing worldwide. Outdoor activity is one of the most important environmental factors for myopia control. Here we show that violet light (VL, 360–400 nm wavelength) suppresses myopia progression. First, we confirmed that VL suppressed the axial length (AL) elongation in the chick myopia model. Expression microarray analyses revealed that myopia suppressive gene EGR1 was upregulated by VL exposure. VL exposure induced significantly higher upregulation of EGR1 in chick chorioretinal tissues than blue light under the same conditions. Next, we conducted clinical research retrospectively to compare the AL elongation among myopic children who wore eyeglasses (VL blocked) and two types of contact lenses (partially VL blocked and VL transmitting). The data showed the VL transmitting contact lenses suppressed myopia progression most. These results suggest that VL is one of the important outdoor environmental factors for myopia control. Since VL is apt to be excluded from our modern society due to the excessive UV protection, VL exposure can be a preventive strategy against myopia progression.

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“…Previously, the MAPK cascade (also known as the MEK/ERK [mitogen-activated protein kinase/extracellular-regulated kinase] pathway) has been demonstrated to be activated by the insulin receptor, which is involved in stimulating myopia in chickens [23] . MAPK phosphatase 2 is downregulated in the retina of chicks with form-deprivation myopia [24] . MAPK10 may be downregulated by the upregulated miRNAs, affecting the MAPK cascade in myopia.…”

Section: Functional Annotation Resultsmentioning

confidence: 99%

Potentially Important MicroRNAs in Form-Deprivation Myopia Revealed by Bioinformatics Analysis of MicroRNA Profiling

Mei

Wang²,

Chen³

et al. 2017

Ophthalmic Res

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Objective: This study aimed to identify differentially expressed microRNAs (miRNAs) and to explore their potential roles in form-deprivation myopia. Methods: The microarray data set GSE58124 of miRNAs was downloaded from the Gene Expression Omnibus, and form-deprivation myopia was induced in C57BL/6J mice over the right eye; the contralateral, left eyes were used as controls. Differential expression analysis was done using the LIMMA package. miRDB was used to predict targets for miRNAs. The target genes were put into DAVID to identify significant pathways and biological processes of miRNAs. A functionally collaborative network was constructed using Cytoscape. Result: In total, 24 and 20 upregulated miRNAs, respectively, were screened out in retina and whole-eye tissue. However, there was no dramatic expression change of miRNAs in sclera tissue. By taking intersections, 8 common upregulated miRNAs were obtained in both the retina and the whole-eye samples. According to miRDB, 1,805 target genes were screened out for the 8 differentially expressed miRNAs, including MAPK10 (mitogen-activated protein kinase 10). The functionally collaborative network revealed that “regulation of transcription” was significantly enriched. The pathways “Axon guidance” and “TGF-β signaling pathway” were also enriched. Importantly, miR-466h-5p and miR-466j were significantly enriched in some synaptic transmission-related biological processes. Conclusion: This study identified an upregulation of 8 miRNAs, which may function by disturbing their enriched pathways or biological processes in the progression of myopia.

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Form-Deprivation Myopia in Chick Induces Limited Changes in Retinal Gene Expression

Cited by 62 publications

References 66 publications

RPE and Choroid Mechanisms Underlying Ocular Growth and Myopia

RPE and Choroid Mechanisms Underlying Ocular Growth and Myopia

Violet Light Exposure Can Be a Preventive Strategy Against Myopia Progression

Potentially Important MicroRNAs in Form-Deprivation Myopia Revealed by Bioinformatics Analysis of MicroRNA Profiling

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