Formalin Fixation for Electron Microscopy: A Re-evaluation

Carson, Freida L.; Martin, J. Holmes; Lynn, Joseph A.

doi:10.1093/ajcp/59.3.365

Cited by 368 publications

(152 citation statements)

References 0 publications

Supporting

Mentioning

144

Contrasting

Unclassified

Order By: Relevance

“…The liver was removed, the liver mass (LM) and liver volume (LV) were measured using immersion method (Scherle 1970). Liver specimens were separated into several minor fragments kept for 48 h at room temperature in a fixative (modified Millonig's phosphate-buffered formalin, pH 7.4) (Carson et al 1973), embedded in paraffin, sectioned at 5µm thickness, and the sections were stained with Masson's trichrome.…”

Section: Methodsmentioning

confidence: 99%

Hepatic stereology of schistosomiasis mansoni infected-mice fed a high-fat diet

Neves

Alencar

Águila

et al. 2006

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

High-fat diets induce weight gain and fatty liver in wild-type mice. Schistosomiasis mansoni infection also promotes hepatic injury. This study was designed to quantify hepatic alterations in schistosomiasis mansoni infected mice fed a high-fat diet (IHFC) or standard chow (ISC), uninfected mice fed a high-fat diet (HFC) or standard chow (SC). Mice were sacrificed during early infection (9 weeks from exposure). The following hepatic biometry and the stereology parameters were determined: volume density (hepatocytes [h], sinusoids [s], steatosis [st] and hepatic fibrosis [hf]); numerical density (hepatocyte nuclei -Nv[h]); absolute number of total hepatocyte N[h], normal hepatocyte N[nh], and binucleated hepatocyte N[bh], percentage of normal hepatocyte P[nh] and binucleated hepatocyte P[bh]. IHFC and HFC groups exhibited TC, HDL-C, LDL-C, and body mass significantly greater (p < 0.05) than control group. No significant differences were found regards liver volume (p = 0.07). Significant differences were observed regards P[nh] (p = 0.0045), P[bh] (p = 0.0045), Nv[h] (p = 0.0006), N[h] (p = 0.0125), N[bh] (p = 0.0164) and N[nh] (p = 0.0078). IHFC mice group presented 29% of binucleated hepatocytes compared to HFC group (19%), ISC group (17%) and SC (6%

show abstract

Section: Methodsmentioning

confidence: 99%

Hepatic stereology of schistosomiasis mansoni infected-mice fed a high-fat diet

Neves

Alencar

Águila

et al. 2006

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

show abstract

“…Histopathological analysis -Liver fragments taken from the right lobule of each animal, was fixed in Carson's modified Millonig's phosphate-buffered formalin, pH 7.4 (Carson's formalin-Millonig) (Carson et al 1973) and embedded in paraffin. Sections (5 µm) were stained with hematoxilin and eosin (H&E); PAS-Alcian Blue, pH = 1.0 and 2.5; Lennert's Giemsa (Lennert 1978); Gomori's reticulin stains, Sirius red pH = 10.2 (eosinophils) (Bogomoletz 1980, Luque & Montes 1989, Vale et al 1997); Masson's trichrome, Alcian blue/safranin (mast cell subpopulation) (Strobel et al 1981) and Picrosirius ± polarization microscopy (Junqueira et al 1979) and Weigert resorcin-fuchsin ± oxidation with potassium peroxymonosulfate (Oxone® monopersulfate compound, Sigma 22,803-6).…”

Section: Methodsmentioning

confidence: 99%

Natural Schistosoma mansoni infection in Nectomys squamipes: histopathological and morphometric analysis in comparison to experimentally infected N. squamipes and C3H/He mice

Costa-Silva

Rodrigues-Silva²,

Hulstijn

et al. 2002

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

show abstract

“…Body weight and spleen weight were registered for each animal, and the relationship between spleen weight and body weight (spleen index) was determined. The various organs were fixed in Milloning formalin 10 and paraffin embedded. Five micrometer thick (5m) sections of the heart, skeletal muscle, liver, spleen and lymph nodes were stained with Hematoxylin and Eosin.…”

Section: Experimental Animalsmentioning

confidence: 99%

Treatment with benznidazole in association with immunosuppressive drugs in mice chronically infected with Trypanosoma cruzi: investigation into the possible development of neoplasias

Andrade

Mesquita

Jambeiro

et al. 2003

Rev. Soc. Bras. Med. Trop.

View full text Add to dashboard Cite

Benznidazole is recommended in Brazil for the treatment of Trypanosoma cruzi infection in acute and early chronic phases of Chagas' disease. Observations by others have indicated a higher incidence of neoplasias in immunosuppressed patients, presenting Chagas' disease reactivation, submitted to treatment with benznidazole. In the present study, we investigated whether there is a potentiation in the generation of lymphomas in chronically infected mice, treated with immunosuppressive drugs and benznidazole. For this, 142 Swiss mice chronically infected with the 21 SF strain of T. cruzi and 72 normal Swiss mice were used. Both infected and normal mice were divided into experimental groups and submitted to one of the following treatment regimens: benznidazole alone; immunosuppressive drugs (azathioprine, betamethasone and cyclosporin); a combination of immunosuppressive drugs and benznidazole; and untreated controls. In the infected group treated with benznidazole, one mouse developed a non-Hodgkin's lymphoma. This finding has been interpreted as a spontaneous tumor of mice. The study of the chronically infected mice treated with the combination of immunosuppressive drugs and benznidazole demonstrated an absence of lymphomas or other neoplasias. These findings support the indication of benznidazole, as the drug of choice, for immunosuppressed patients that develop a reactivation of Chagas' disease.

show abstract

Formalin Fixation for Electron Microscopy: A Re-evaluation

Cited by 368 publications

References 0 publications

Hepatic stereology of schistosomiasis mansoni infected-mice fed a high-fat diet

Hepatic stereology of schistosomiasis mansoni infected-mice fed a high-fat diet

Natural Schistosoma mansoni infection in Nectomys squamipes: histopathological and morphometric analysis in comparison to experimentally infected N. squamipes and C3H/He mice

Treatment with benznidazole in association with immunosuppressive drugs in mice chronically infected with Trypanosoma cruzi: investigation into the possible development of neoplasias

Contact Info

Product

Resources

About