Formalin-fixed tumor cells effectively induce antitumor immunity both in prophylactic and therapeutic conditions

Obata, Chikage; Zhang, Manxin; Moroi, Yoichi; Hisaeda, Hajime; Tanaka, Keiji; Murata, Shigeo; Furue, Masutaka; Himeno, Kunisuke

doi:10.1016/j.jdermsci.2004.02.003

Cited by 9 publications

(7 citation statements)

References 36 publications

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“…The same finding about this protein has been reported in previous studies (17-20). An alternative name of PSME1 is PA28 alpha that encodes the alpha subunit of the 11S regulator.…”

Section: Discussionsupporting

confidence: 91%

“…The other essential function of the proteasome is the processing of class I major histocompatibility complex peptides, namely immunoprotea-some. The results of previous studies (17-20) have revealed that the noticeable function of PSME1 was immunoregulation, which, to some extent, could enhance anticancer immunological reaction. The relationship between PSME1 and some cancers has been reported recently.…”

Section: Discussionmentioning

confidence: 96%

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Comparative proteomics analysis of chronic atrophic gastritis: changes of protein expression in chronic atrophic gastritis without Helicobacter pylori infection

Zhang

Hou

et al. 2012

Braz J Med Biol Res

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Chronic atrophic gastritis (CAG) is a very common gastritis and one of the major precursor lesions of gastric cancer, one of the most common cancers worldwide. The molecular mechanism underlying CAG is unclear, but its elucidation is essential for the prevention and early detection of gastric cancer and appropriate intervention. A combination of two-dimensional gel electrophoresis and mass spectrometry was used in the present study to analyze the differentially expressed proteins. Samples from 21 patients (9 females and 12 males; mean age: 61.8 years) were used. We identified 18 differentially expressed proteins in CAG compared with matched normal mucosa. Eight proteins were up-regulated and 10 down-regulated in CAG when compared with the same amounts of proteins in individually matched normal gastric mucosa. Two novel proteins, proteasome activator subunit 1 (PSME1), which was down-regulated in CAG, and ribosomal protein S12 (RPS12), which was up-regulated in CAG, were further investigated. Their expression was validated by Western blot and RT-PCR in 15 CAG samples matched with normal mucosa. The expression level of RPS12 was significantly higher in CAG than in matched normal gastric mucosa (P < 0.05). In contrast, the expression level of PSME1 in CAG was significantly lower than in matched normal gastric mucosa (P < 0.05). This study clearly demonstrated that there are some changes in protein expression between CAG and normal mucosa. In these changes, down-regulation of PSME1 and up-regulation of RPS12 could be involved in the development of CAG. Thus, the differentially expressed proteins might play important roles in CAG as functional molecules.

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“…The same finding about this protein has been reported in previous studies (17-20). An alternative name of PSME1 is PA28 alpha that encodes the alpha subunit of the 11S regulator.…”

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 96%

Comparative proteomics analysis of chronic atrophic gastritis: changes of protein expression in chronic atrophic gastritis without Helicobacter pylori infection

Zhang

Hou

et al. 2012

Braz J Med Biol Res

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“…Conformational changes in protein molecules which lead to the appearance of cryptic self-epitopes might be induced either by heat [14] or by chemicals such as formaldehyde [15]. We therefore compared the growth of live B16 cells in vivo, followed by immunization with B16 cells either with irradiation plus heat or with irradiation plus rhododendrol.…”

Section: Resultsmentioning

confidence: 99%

T-Cell Responses to Tyrosinase-Derived Self-Peptides in Patients with Leukoderma Induced by Rhododendrol: Implications for Immunotherapy Targeting Melanoma

Takagi

Kawano²,

Nakamura³

et al. 2015

Dermatology

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Background: Rhododendrol, a phenolic compound contained in lightening/whitening cosmetics, can bind and inhibit tyrosinase and was reported to induce leukoderma in Japan. Only 2% of the cosmetics users are affected, and tacrolimus is effective in treatment of the condition. Objective: To test the hypothesis that the disease is an autoimmune disorder. Methods: Short-term T-cell lines were established using peripheral blood mononuclear cells from 8 patients with human melanoma-associated and tyrosinase-derived synthetic peptides. The effects of rhododendrol on melanoma immunization were also examined. Results: Seven out of 8 patients were positive for HLA-DR4. Both class I- and class II-restricted and tyrosinase peptide-specific T-cell responses were observed. Immunization of mice with rhododendrol-treated and irradiated B16 melanoma cells successfully delayed the growth of melanoma cells in vivo. Conclusion: Rhododendrol-induced leukoderma is an autoimmune disorder, with rhododendrol as an environmental factor and HLA-DR4 as a genetic factor. Rhododendrol might be effective in treating melanomas.

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“…Various methods have been developed for antigen preparation, including the use of γ-irradiated tumor cells, formalin-fixed cells (7,8), glutaraldehyde-fixed cells (9) and frozen/thawed cells (10). As cellular proteins are better preserved in ethanol-fixed tissue (8,11), ethanol-fixed RM-1 cells were selected as a source of tumor antigens for immunotherapy.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of prostate cancer growth by immunization with a GM-CSF-modified mouse prostate cancer RM‑1 cell vaccine in a novel murine model

Xia

Luo

2017

Oncol Lett

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Abstract.Advanced prostate cancer is difficult to treat owing to a lack of effective approaches for disrupting immune tolerance. C57BL/6 male and female mice implanted with viable RM-1 cells represent a novel murine model of advanced prostate cancer for studying antitumor effects following immunization with a granulocyte-macrophage colony-stimulating factor (GM-CSF)-modified RM-1 cell vaccine, which has been described previously. In vitro cytotoxic activity and cytokine secretion experiments were conducted to investigate the antitumor response. The cytotoxicity profile of splenocytes from female mice immunized against RM-1 cells primarily involved cytotoxic T lymphocyte (CTL) lysis and, to a lesser extent, natural killer (NK) cell lysis. NK cell lysis was also observed in males, which exhibited no evidence of CTL lysis. The secretion of interferon-γ in the GM-CSF-modified cell vaccine group was significantly increased compared with the other groups. The level of interleukin-4 was low. To investigate the antitumor immune response further, cluster of differentiation 4 (CD4) T cells and CD8 T cells were analyzed in the spleens and tumors of female mice receiving the GM-CSF-modified RM-1 cell vaccine. Unlike female mice, males exhibited the highest proportion of NK cells in the spleen. NK cells were not detected in the tumor tissue in any of the groups. The difference between the sexes may explain the specificity of the immune response, as females are intolerant to prostate antigens whereas males are. This model is clinically relevant as it translates to human immunology and offers an effective and convenient method for studying immunotherapy for prostate cancer.

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Formalin-fixed tumor cells effectively induce antitumor immunity both in prophylactic and therapeutic conditions

Cited by 9 publications

References 36 publications

Comparative proteomics analysis of chronic atrophic gastritis: changes of protein expression in chronic atrophic gastritis without Helicobacter pylori infection

Comparative proteomics analysis of chronic atrophic gastritis: changes of protein expression in chronic atrophic gastritis without Helicobacter pylori infection

T-Cell Responses to Tyrosinase-Derived Self-Peptides in Patients with Leukoderma Induced by Rhododendrol: Implications for Immunotherapy Targeting Melanoma

Inhibition of prostate cancer growth by immunization with a GM-CSF-modified mouse prostate cancer RM‑1 cell vaccine in a novel murine model

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