Formation and analysis of topographical domains between lipid membranes tethered by DNA hybrids of different lengths

Chung, Minsub; Koo, Bon Jun; Boxer, Steven G.

doi:10.1039/c2fd20108a

Cited by 39 publications

(45 citation statements)

References 30 publications

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“…1b). Among others, this strategy has been explored and optimised by Höök and coworkers [13,[58][59][60] and Boxer and coworkers [61][62][63][64][65][66] for the case of SLBs as well as liposomes. Tested anchor moieties include cholesterol, lipids, tocopherol or carbon chains, as recently reviewed by Lopez and Liu [67].…”

Section: Functionalised Lipid Membranesmentioning

confidence: 99%

Programmable interactions with biomimetic DNA linkers at fluid membranes and interfaces

2019

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At the heart of the structured architecture and complex dynamics of biological systems are specific and timely interactions operated by biomolecules. In many instances, biomolecular agents are spatially confined to flexible lipid membranes where, among other functions, they control cell adhesion, motility and tissue formation. Besides being central to several biological processes, multivalent interactions mediated by reactive linkers confined to deformable substrates underpin the design of syntheticbiological platforms and advanced biomimetic materials. Here we review recent advances on the experimental study and theoretical modelling of a heterogeneous class of biomimetic systems in which synthetic linkers mediate multivalent interactions between fluid and deformable colloidal units, including lipid vesicles and emulsion droplets. Linkers are often prepared from synthetic DNA nanostructures, enabling full programmability of the thermodynamic and kinetic properties of their mutual interactions. The coupling of the statistical effects of multivalent interactions with substrate fluidity and deformability gives rise to a rich emerging phenomenology that, in the context of self-assembled soft materials, has been shown to produce exotic phase behaviour, stimuli-responsiveness, and kinetic programmability of the self-assembly process. Applications to (synthetic) biology will also be reviewed.

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Section: Functionalised Lipid Membranesmentioning

confidence: 99%

Programmable interactions with biomimetic DNA linkers at fluid membranes and interfaces

2019

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“…3,48,52,84,85,90,91 There are two broad foci for the information one would like to obtain in such systems. One focus addresses how the additive resides in the bilayer.…”

Section: Towards More Complex Membranesmentioning

confidence: 99%

Introductory Lecture: Basic quantities in model biomembranes

2013

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One of the many aspects of membrane biophysics dealt with in this Faraday Discussion regards the material moduli that describe energies at a supramolecular level. This introductory lecture first critically reviews differences in reported numerical values of the bending modulus KC, which is a central property for the biologically important flexibility of membranes. It is speculated that there may be a reason that the shape analysis method tends to give larger values of KC than the micromechanical manipulation method or the more recent X-ray method that agree very well with each other. Another theme of membrane biophysics is the use of simulations to provide exquisite detail of structures and processes. This lecture critically reviews the application of atomic level simulations to the quantitative structure of simple single component lipid bilayers and diagnostics are introduced to evaluate simulations. Another theme of this Faraday Discussion was lateral heterogeneity in biomembranes with many different lipids. Coarse grained simulations and analytical theories promise to synergistically enhance experimental studies when their interaction parameters are tuned to agree with experimental data, such as the slopes of experimental tie lines in ternary phase diagrams. Finally, attention is called to contributions that add relevant biological molecules to bilayers and to contributions that study the exciting shape changes and different non-bilayer structures with different lipids.

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“…A second, but quite different, form of domain formation has also been demonstrated within DNA-functionalized membranes [103]. By using different length DNA tethers (24mers combined with either 48mers or 72mers) between a surface-supported lipid bilayer and a second tethered membrane, the laterally mobile DNA strands self-sort into domains of equal DNA length in order to minimise the total curvature energy of the tethered bilayer (Fig.…”

Section: Breaking Symmetry: Phase Separation and Aspherical Structmentioning

confidence: 99%

Application of nucleic acid–lipid conjugates for the programmable organisation of liposomal modules

Beales

Vanderlick

2014

Advances in Colloid and Interface Science

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Formation and analysis of topographical domains between lipid membranes tethered by DNA hybrids of different lengths

Cited by 39 publications

References 30 publications

Programmable interactions with biomimetic DNA linkers at fluid membranes and interfaces

Programmable interactions with biomimetic DNA linkers at fluid membranes and interfaces

Introductory Lecture: Basic quantities in model biomembranes

Application of nucleic acid–lipid conjugates for the programmable organisation of liposomal modules

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