Formation and Properties Polymer Nanolayers to Enhance Cell Growth in vitro

Abstract

Three-dimensional (3D) cultures models may more accurate representation of the in vivo environment than two-dimensional (2D) cultures while maintaining the cytoarchitecture of in situ tissue that supports cells differentiation or maturation [1, 2]. Cell adhesion is depended upon surface hydrophilicity, surface charge density, surface morpho logy, specific chemical groups present on the surface of the scaffold [3]. Given that surface chemistry is crucial for the biocompatibility of the nanolayers, specific surface modifications are used with different polymers [4, 5]. In particular, there is an increased interest in the polymeric surfaces which can change their affinity towards proteins and cells under external stimuli [6, 7] and therefore have potential applications in biology and medicine. Despite various investigations, specific and complex mechanisms govern the reactions that occur between the biomaterial and the cellular environment are still incomplete understanding. The objective of this study was to establish and comparison cells line B16F10 viability cultured onto different coatings. We used surfaces obtained by grafting APTES, dextran, albumin and their combinations to the surface of the modified glass plates. Materials and Methods Preparation of coatings. Glass plates (2020) were dipped into 0.2 % (w/w) methanolic solution of (3-aminopropyl)triethoxysilane (APTES) for 24 h. After the incubation, loosely-attached silane molecules were removed with methanol in Soxhlet's apparatus. Then the plates functionalized with APTES

Microarchitectonic of Decellularized Bovine Pericardium Matrix

“…However, residual glutaraldehyde can lead to cytotoxicity and calcification of the bioimplant with long-term observation [15]. In this study for the stabilizing the matrix fibers we used the soluble carbodiimide EDC (1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide) and an organic substance derived from pyrrolidine NHS (N-hydroxysuccinimide), which are successfully used in medicine in the treatment of catarrh in vivo [16,17]. EDC activates the carboxyl group in the amino acid by converting it to the intermediate isourea, which in turn binds to lysine residues or other available primary amines.…”

The Effect of Sterilization on the Bovine Pericardium Scaffold Decellularized By the Glutaraldehyde-Free Technology

“…However, residual glutaraldehyde can lead to cytotoxicity, inflammation and calcification of the bioimplant with long-term observation [15]. In this study for the stabilizing the matrix fibers we used the soluble carbodiimide EDC (1-Ethyl-3-(3dimethylaminopropyl) carbodiimide) and an organic substance derived from pyrrolidine NHS (Nhydroxysuccinimide), which are successfully used in medicine in the treatment of catarrh in vivo [16,17]. EDC is the most popular zero-length crosslinker for biochemical conjugations because it can efficiently form conjugates between two protein molecules, between a protein and a peptide, and between proteins and oligonucleotides, and with small molecules.…”

Different mice inbred strains humoral immune response against human prostate-specific antigen

“…From the biotechnological position, the starting point for any serological method is the immune response. And hence one of the most important fundamental grounds of the creation of serological diagnostic kits is the understanding of the regularity and features of the immune response against the certain antigen [8][9][10][11]. The most appropriate is specific monoclonal antibodies (McAbs), as they, unlike polyclonal antibodies are characterized by exceptional specificity, homogeneity and possibility of obtaining in unlimited quantities.…”