Formation of a test system and the choice of test criteria when biotesting natural waters

Гончарук, В. В.; Сыроешкин, А. В.; Kovalenko, V. F.; Zlatskiy, I. A.

doi:10.3103/s1063455x16060084

Cited by 16 publications

(9 citation statements)

References 3 publications

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“…However, we can state that the upward transport of bacterial DNA is one explanation of the close similarity. Aerosol transport, including living organisms, from the Earth's surface to the troposphere is well described [ 20 , 21 , 32 – 35 ]. The examples of bacterial genera, which can be attributed to the aerobes of troposphere, indicating the transport mechanisms from the surface of the ocean and land, are presented in the third line of Table 2 .…”

Section: Discussionmentioning

confidence: 99%

The DNA of Bacteria of the World Ocean and the Earth in Cosmic Dust at the International Space Station

Гребенникова

Сыроешкин

Shubralova

et al. 2018

The Scientific World Journal

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Cosmic dust samples from the surface of the illuminator of the International Space Station (ISS) were collected by a crew member during his spacewalk. The sampler with tampon in a vacuum container was delivered to the Earth. Washouts from the tampon's material and the tampon itself were analyzed for the presence of bacterial DNA by the method of nested PCR with primers specific to DNA of the genus Mycobacteria, DNA of the strains of capsular bacteria Bacillus, and DNA encoding 16S ribosomal RNA. The results of amplification followed by sequencing and phylogenetic analysis indicated the presence of the bacteria of the genus Mycobacteria and the extreme bacterium of the genus Delftia in the samples of cosmic dust. It was shown that the DNA sequence of one of the bacteria of the genus Mycobacteria was genetically similar to that previously observed in superficial micro layer at the Barents and Kara seas' coastal zones. The presence of the wild land and marine bacteria DNA on the ISS suggests their possible transfer from the stratosphere into the ionosphere with the ascending branch of the global electric circuit. Alternatively, the wild land and marine bacteria as well as the ISS bacteria may all have an ultimate space origin.

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Section: Discussionmentioning

confidence: 99%

The DNA of Bacteria of the World Ocean and the Earth in Cosmic Dust at the International Space Station

Гребенникова

Сыроешкин

Shubralova

et al. 2018

The Scientific World Journal

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“…D. magna is a standard sensitive species of crustaceans used in bioassay of water [61]. Laboratory culture of D. magna was established from gravid females originally acquired from Laboratory of biotesting and bioindication (Dumanskii Institute of Colloid and Water Chemistry, Ukraine).…”

Section: Daphnia Magna Culture Assaysmentioning

confidence: 99%

“…Laboratory culture of D. magna was established from gravid females originally acquired from Laboratory of biotesting and bioindication (Dumanskii Institute of Colloid and Water Chemistry, Ukraine). The culture was maintained in synthetic laboratory water [61]. D. magna were kept under constant light/dark (16:8 hours) and temperature (20-22ºC) conditions and were fed a slurry of yeast, with water renewal every two days.…”

Section: Daphnia Magna Culture Assaysmentioning

confidence: 99%

“…D. magna were kept under constant light/dark (16:8 hours) and temperature (20-22ºC) conditions and were fed a slurry of yeast, with water renewal every two days. All bioassay procedures were performed according to standard methods [61,62].…”

Section: Daphnia Magna Culture Assaysmentioning

confidence: 99%

“…Classical bioassays were chosen for assessing the effect of HS on living organisms, namely, daphnia (Daphnia magna) [61] -the crustacean object of the invertebrate freshwater fauna, and the representative of monocotyledonous plants -bulb onion (Allium cepa L.) [62].…”

Section: Introductionmentioning

confidence: 99%

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Correlation Biological Activity and Physicochemical Properties of Humic Acids in Aqueous Solutions

Доленко

Kravchenko

Zlatskiy

2020

Pol. J. Environ. Stud.

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Objective. The biological activity of humic acids (HA), considering them as open non-equilibrium systems, in which properties are determined by the state or level of organization of the system.Methods. The dependence of physicochemical properties of HA on their concentration (optical and sorption properties, ζ-potential and electrical conductivity, average size of optical homogeneities) were analyzed by several methods: optical microscopy; low-angle laser light scattering (LALLS) method on a Zetasizer Nano ZS analyzer; absorption on a Shimadzu UV-3600 spectrophotometer; chemical analysis on an ICP-QMS Agilent 7500CE spectrometer. Biological activity of HA was studied on the crustacean Daphnia magna and the monocotyledonous plants Allium cepa L.Results. It was established that the nature of the HA effect on biological objects is non-linear and has an extremum at 1 mg•L -1 . It was shown that the mineralization of solutions affects the biological activity of HA. So in the presence of the addition of mineral salts, for D. magna the effect of HA is insensible, while for A. cepa L. it changes its direction. In the absence of mineral salts at HA concentrations < 5-10 mg•L -1 , a sharp drop in the survival of D. magna and suppression of root growth of A. cepa L. was observed, and in saline solution -stimulation of root growth. It has been established that the concentration range of 5-10 mg•L -1 was also critical for concentration changes in a number of physicochemical parameters of aqueous solutions of HA, which makes it possible to consider it a phase transition characterized by new properties.Findings. It was shown that changes in various properties of aqueous solutions of HA, including biological activity, were characterized by non-linear concentration dependencies with the presence of intermittent changes indicating qualitative rearrangements in the system.

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D/H control of chemical kinetics in water solutions under low deuterium concentrations

Сыроешкин

Pleteneva

Uspenskaya

et al. 2019

Chemical Engineering Journal

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Formation of a test system and the choice of test criteria when biotesting natural waters

Cited by 16 publications

References 3 publications

The DNA of Bacteria of the World Ocean and the Earth in Cosmic Dust at the International Space Station

The DNA of Bacteria of the World Ocean and the Earth in Cosmic Dust at the International Space Station

Correlation Biological Activity and Physicochemical Properties of Humic Acids in Aqueous Solutions

D/H control of chemical kinetics in water solutions under low deuterium concentrations

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