Formulating co-loaded nanoliposomes with gallic acid and quercetin for enhanced cancer therapy

Al-Samydai, Ali; Al Qaraleh, Moath; Al Azzam, Khaldun M.; Mayyas, Amal; Nsairat, Hamdi; Abu Hajleh, Maha N.; Al-Halaseh, Lidia K.; Al-Karablieh, Nehaya; Akour, Amal; Alshaik, Fatima; Alshaer, Walhan

doi:10.1016/j.heliyon.2023.e17267

Cited by 23 publications

(2 citation statements)

References 47 publications

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“…The percentage of encapsulated 6-gingerol was determined through degradation of encapsulation NLs through the addition of acetonitrile and centrifugation at 7000 rpm for 10 min, followed by 10 min of sonication at 35 C, and further centrifugation at 12,000 rpm for 10 min. The supernatant was filtered using a 0.45-μm syringe filter (Al-Samydai et al, 2021;Al-Samydai et al, 2023), and the concentration of the encapsulated 6-gingerol was measured using an HPLC system (UV Detector at 280-282 nm, HPLC Nucleodur-C18 Column; 250 Â 4.6 mm, 5 μm), at a flow rate of 1 mL/min and column temperature of 40 C using a 10-μL injection volume connected to computer software Labsolution. The mobile phase used was HPLC-grade methanol:water (at a ratio of 60:40).…”

Section: Encapsulation Efficiency and Drug Loadingmentioning

confidence: 99%

Encapsulation of gingerol into nanoliposomes: Evaluation of in vitro anti‐inflammatory and anti‐cancer activity

Alshaikh,

Al‐Samydai,

Issa

et al. 2024

Biomedical Chromatography

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Nanoliposomes (NLs) are ideal carriers for delivering complex molecules and phytochemical products, but ginger by‐products, despite their therapeutic benefits, have poor bioavailability due to their low water solubility and stability. Crude ginger extracts (CGEs) and 6‐gingerol were individually encapsulated within NLs for in vitro activity assessment. In vitro evaluation of anti‐proliferative and anti‐inflammatory properties of encapsulated 6‐gingerol and CGE was performed on healthy human periodontal ligament (PDL) fibroblasts and MDA‐MB‐231 breast cancer cells. Encapsulation efficiency and loading capacity of 6‐gingerol reached 25.23% and 2.5%, respectively. NLs were found stable for up to 30 days at 4°C with a gradual load loss of up to 20%. In vitro cytotoxic effect of encapsulated 6‐gingerol exceeded 70% in the MDA‐MB‐231 cell line, in a comparable manner with non‐encapsulated 6‐gingerol and CGE. The effect of CGE with an IC50 of 3.11 ± 0.39, 7.14 ± 0.80, and 0.82 ± 0.55 μM and encapsulated 6‐gingerol on inhibiting IL‐8 was evident, indicating its potential anti‐inflammatory activity. Encapsulating 6‐gingerol within NLs enhanced its stability and facilitated its biological activity. All compounds, including vitamin C, were equivalent at concentrations below 2 mg/mL, with a slight difference in antioxidant activity. The concentrations capable of inhibiting 50% of 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) substrate were comparable.

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Section: Encapsulation Efficiency and Drug Loadingmentioning

confidence: 99%

Encapsulation of gingerol into nanoliposomes: Evaluation of in vitro anti‐inflammatory and anti‐cancer activity

Alshaikh,

Al‐Samydai,

Issa

et al. 2024

Biomedical Chromatography

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“…Plant-derived phenolic molecules are the most significant active compounds involved in anti-colorectal cancer actions [ 11 , 12 ]. Oleuropein (OLR) is the most efficient phenolic molecule for reducing cancer cell viability in various categories of cancer as breast, gastric and kidney [ 13 , 14 ].…”

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confidence: 99%

Oleuropein impact on colorectal cancer

Nsairat,

Jaber,

Faddah

et al. 2024

Future Science OA

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Colorectal cancer (CRC) is considered the third most common cancer in the world. In Mediterranean region, olives and olive oil play a substantial role in diet and medical traditional behaviors. They totally believe that high consumption of olive products can treat a wide range of diseases and decrease risk of illness. Oleuropein is the main active antioxidant molecule found in pre-mature olive fruit and leaves. Recently, it has been demonstrated that oleuropein is used in cancer therapy as an anti-proliferative and apoptotic agent for some cancer cells. In this review, we would like to explore the conclusive effects of oleuropein on CRC with respect to in vitro and in vivo studies.

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Preparation, Characterization, and Anticancer Activity of PEGylated Nano Liposomal Loaded with Rutin against Human Carcinoma Cells (HT‐29)

Al‐Ekaid,

Al‐Samydai,

Al‐deeb

et al. 2023

Chemistry & Biodiversity

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The abstract discusses the development of rutin‐loaded nanoliposomes and their anti‐colorectal cancer activity against human carcinoma cells (HT‐29). The study characterizes the nanoliposomes using the thin‐film hydration method and analyzes their size, charge, and polydispersity index. The encapsulation efficiency and drug loading ability of rutin at different concentrations were investigated. The nanoliposomes were found to be stable for up to one month at 4 ºC and showed sustained drug release for up to 24 hours. The anti‐cancer activity of the rutin‐loaded nanoliposomes was found to be concentration‐dependent and significantly improved compared to free rutin. PEGylated nanoliposomes with rutin (1.8 mg/ml) showed the highest encapsulation efficiency and drug loading ability, along with improved selectivity against cancer cells. Overall, the study provides important insights into the potential use of rutin‐loaded nanoliposomes for the treatment of colorectal cancer.

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Formulating co-loaded nanoliposomes with gallic acid and quercetin for enhanced cancer therapy

Cited by 23 publications

References 47 publications

Encapsulation of gingerol into nanoliposomes: Evaluation of in vitro anti‐inflammatory and anti‐cancer activity

Encapsulation of gingerol into nanoliposomes: Evaluation of in vitro anti‐inflammatory and anti‐cancer activity

Oleuropein impact on colorectal cancer

Preparation, Characterization, and Anticancer Activity of PEGylated Nano Liposomal Loaded with Rutin against Human Carcinoma Cells (HT‐29)

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