Formulation and Antibacterial Activity Evaluation of Quaternized Aminochitosan Membrane for Wound Dressing Applications

Abstract

Much attention has been paid to chitosan biopolymer for advanced wound dressing owing to its exceptional biological characteristics comprising biodegradability, biocompatibility and respectable antibacterial activity. This study intended to develop a new antibacterial membrane based on quaternized aminochitosan (QAMCS) derivative. Herein, aminochitosan (AMCS) derivative was quaternized by N-(2-Chloroethyl) dimethylamine hydrochloride with different ratios. The pre-fabricated membranes were characterized by several analysis tools. The results indicate that maximum surface potential of +42.2 mV was attained by QAMCS3 membrane compared with +33.6 mV for native AMCS membrane. Moreover, membranes displayed higher surface roughness (1.27 ± 0.24 μm) and higher water uptake value (237 ± 8%) for QAMCS3 compared with 0.81 ± 0.08 μm and 165 ± 6% for neat AMCS membranes. Furthermore, the antibacterial activities were evaluated against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus cereus. Superior antibacterial activities with maximum inhibition values of 80–98% were accomplished by QAMCS3 membranes compared with 57–72% for AMCS membrane. Minimum inhibition concentration (MIC) results denote that the antibacterial activities were significantly boosted with increasing of polymeric sample concentration from 25 to 250 µg/mL. Additionally, all membranes unveiled better biocompatibility and respectable biodegradability, suggesting their possible application for advanced wound dressing.

Ethnopharmacological Survey and Comparative Study of the Healing Activity of Moroccan Thyme Honey and Its Mixture with Selected Essential Oils on Two Types of Wounds on Albino Rabbits

“…In comparison, the most susceptible bacteria to Rosmarinus officinalis were, in order, L. monocytogenes, S. aureus, E. coli , and S. typhimurium . Indeed, the MIC test is required to evaluate the novel composite’s antibacterial properties traditionally used in wound healing [ 75 ]. In order to evaluate the nature of the antibacterial molecules, MBC/MIC values listed in Table 7 were calculated.…”

Metronidazole Topically Immobilized Electrospun Nanofibrous Scaffold: Novel Secondary Intention Wound Healing Accelerator

“…At both days 7 and 14 the wound tissue sample was removed from the rats after sacrificing using high dose of anesthesia followed by cervical dislocation for histopathological examination. The excised skin was fixed in 10% formalin, stained with hematoxylin and eosin (H&E) for estimation of epithelialization and keratinization and Masson’s trichrome stainings (MTS) for estimation of collagen deposition under an optical microscope [ 84 , 85 , 86 ].The wound area was calculated with the help of digital caliber and the % contraction of the wound area was calculated using the following equation [ 87 ]. …”

Sustainable adsorptive removal of antibiotic residues by chitosan composites: An insight into current developments and future recommendations

“…Eltaweil et al, 2021a , Eltaweil et al, 2021b , Eltaweil et al, 2021c , Hosny et al, 2021a , Hosny et al, 2021b , Li et al, 2018a , Li et al, 2018b , Liu et al, 2020a , Liu et al, 2020b , Omer et al, 2021a , Omer et al, 2021b , Omer et al, 2021c , Rahman and Varshney, 2021a , Rahman and Varshney, 2021b .…”