Formulation and Evaluation of Phytosome-Loaded Maltodextrin-Gum Arabic Microsphere System for Delivery of Camellia sinensis Extract

Anwar, Effionora; Farhana, Nadira

doi:10.5530/jyp.2018.2s.11

Cited by 35 publications

(18 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Khomendra et al demonstrated that nanovesicles formulated from pepper (Bhut jolokia) have potential as an anti-arthritic medicine [21], and Raimondo et al isolated nanovesicles from lemon (Citrus limon) and showed that they exert anticancer activity [22]. Moreover, combined plant extracts and lipid nanovesicles have recently been formulated as nanovesicles, called ethosomes or phytosomes, that exhibit increased stability, facilitating their delivery for dermatological disorders [23,24].…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic Effects of Plant Sap-Derived Extracellular Vesicles on Various Tumor Cell Types

Kim

Yoo

Jung

et al. 2020

JFB

View full text Add to dashboard Cite

Edible plants have been widely used in traditional therapeutics because of the biological activities of their natural ingredients, including anticancer, antioxidant, and anti-inflammatory properties. Plant sap contains such medicinal substances and their secondary metabolites provide unique chemical structures that contribute to their therapeutic efficacy. Plant extracts are known to contain a variety of extracellular vesicles (EVs) but the effects of such EVs on various cancers have not been investigated. Here, we extracted EVs from four plants—Dendropanax morbifera, Pinus densiflora, Thuja occidentalis, and Chamaecyparis obtusa—that are known to have cytotoxic effects. We evaluated the cytotoxic effects of these EVs by assessing their ability to selectively reduce the viability of various tumor cell types compared with normal cells and low metastatic cells. EVs from D. morbifera and P. densiflora sap showed strong cytotoxic effects on tumor cells, whereas those from T. occidentalis and C. obtusa had no significant effect on any tumor cell types. We also identified synergistic effect of EVs from D. morbifera and P. densiflora saps on breast and skin tumor cells and established optimized treatment concentrations. Our findings suggest these EVs from plant sap as new candidates for cancer treatment.

show abstract

Section: Introductionmentioning

confidence: 99%

Cytotoxic Effects of Plant Sap-Derived Extracellular Vesicles on Various Tumor Cell Types

Kim

Yoo

Jung

et al. 2020

JFB

View full text Add to dashboard Cite

show abstract

“…The michrosphere of F1 has zeta potential below-30 mV to allow agglomeration between particles to make the particle size larger. Nanoparticles with zeta potential below-30 mV and above+30 mV have good suspension stability because the surface charge prevents aggregation between particles [15]. Zeta potential values F1, F2, and F3 respectively; -28.88±0.81;-32.15±1.29; and-30.20±0.61.…”

Section: Discussionmentioning

confidence: 99%

“…The procedure on this study refers to Anwar et al [15]. The allicin releases from microcapsule carried out in two types of mediums, hydrochloric acid pH 1.2 and phosphate buffer pH 7.4.…”

Section: In Vitro Drug Release Studiesmentioning

confidence: 99%

Preparation and Characterization of Enteric-Coated Delayed-Release Microsphere of Phytosome Loading Allicin-Rich Extract

2021

View full text Add to dashboard Cite

Objective: Allicin, a natural organosulfur compound, is the main garlic ingredient, which has extensive pharmacological activities. Its unstable under acidic conditions due to alliinase's inactivation causes the need for preparations that delayed-release in the stomach to maximize allicin absorption. This study aimed to prepare and characterize the enteric-coated microsphere of phytosome loading allicin-rich extract to protect it from gastric acid. Methods: The allicin-rich extract phytosome (ArE-Ps) was prepared and evaluated for characteristics. Microsphere was made in three formulas with different molar ratios of ArE-Ps and Eudragit L30D-55 (1:1; 1:1.5 and 1:2) by spray dry. The three microspheres compared to particle size, entrapment efficiency, and dissolution test in acid and 7.4 pH medium. Results: Optimized ArE-Ps has a size of 251.6 nm, polydispersity index 0.466, zeta potential 34.11, entrapment efficiency of 62.62 %, and specific gravity of 1,005 g/ml. The surface topography of the three formulas shows an almost spherical shape with concave surfaces. The particle size of the microsphere ranges from 215±6.27 nm to 548.8±10.15 nm. Entrapment efficiency increases with an increasing number of polymers with a maximum value of 65.44 % at F3. The results dissolution test in vitro showed no drug release in acidic medium, and drug release occurred at a 7.4 pH medium. Drug release of three microsphere formulations followed the Korsmeyer-Peppas model with a k value of 12.7088±0.1769; 17.9322±1.5621; and 12.958±1.2677; respectively. Conclusion: Based on these results, the polymer's increase in three microsphere formulations can affect characteristics and retain drug release under acidic conditions.

show abstract

“…Phytosomes was prepared 14,15 by using thin layer hydration method with different molar ratio of drug, 30% Soya lecithin and cholesterol. Accurately weighed amount of soya lecithin and cholesterol was dissolved in dichloromethane, while drug was dissolved in methanol.…”

Section: Preparation Of Phytosomesmentioning

confidence: 99%

Formulation of Alkaloid Loaded Phytosomes from Tinospora cordifolia and ex-vivo Intestinal Permeability Study

Thakurx¹,

Patil²

2021

IJPER

View full text Add to dashboard Cite

Background: Phytosomes are newly introduced novel dosage form for improving the bioavailability and therapeutic effect of the herbal drug. The present study aimed to prepare and evaluate the alkaloid loaded phytosome from Tinospora cordifolia. Materials and Methods: Extracts were screened for total content of phenols, flavonoids and alkaloids. The prepared extract was further used for fractionation and dichloromethane fraction (F3) was used to prepare phytosomes. Phytosome was prepared by using thin film hydration method with different ratio of soya lecithin and cholesterol. The evaluation of phytosome was done by particle size and shape, polydispersity index, differential scanning calorimetry and fourier transfer infrared spectroscopy. Further, ex-vivo intestinal permeability study was performed for crude fraction and prepared phytosome to assess the intestinal permeability enhancement. Results: The all standardization parameters showed the results within the standard limits, thus confirming the quality and purity of raw material. The particle size and polydispersity index was found within the acceptable limits. The Differential scanning calorimetry and Fourier transfer infrared spectroscopy studies confirmed that there was no drug interaction with the excipients. The ex-vivo l permeable analysis of the intestines study was found that the phytosome shows enhancement in intestinal permeability than the crude alkaloid fraction. Conclusion: The above results indicated that the prepared alkaloid loaded phytosome shows promising potential in enhancement of intestinal permeability of Tinospora cordifolia.

show abstract

Formulation and Evaluation of Phytosome-Loaded Maltodextrin-Gum Arabic Microsphere System for Delivery of Camellia sinensis Extract

Cited by 35 publications

References 11 publications

Cytotoxic Effects of Plant Sap-Derived Extracellular Vesicles on Various Tumor Cell Types

Cytotoxic Effects of Plant Sap-Derived Extracellular Vesicles on Various Tumor Cell Types

Preparation and Characterization of Enteric-Coated Delayed-Release Microsphere of Phytosome Loading Allicin-Rich Extract

Formulation of Alkaloid Loaded Phytosomes from Tinospora cordifolia and ex-vivo Intestinal Permeability Study

Contact Info

Product

Resources

About