Formulation and in vitro Penetration Study of Transfersomes Gel Containing Gotu Kola Leaves Extract (Centella asiatica L. Urban)

Surini, Silvia; Sarah, Sarah Sarah; Djajadisastra, Joshita

doi:10.5530/jyp.2018.10.8

Cited by 19 publications

(16 citation statements)

References 11 publications

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“…where D is the deformability index, J is the amount of transfersome suspension that passed through the membrane in 5 min (mL), rv is the particle size of the transfersomes that passed through the membrane (nm), and rp is the membrane pore size (nm). 21…”

Section: Deformability Indexmentioning

confidence: 99%

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Formulation and In Vitro Penetration Study of Recombinant Human Epidermal Growth Factor-Loaded Transfersomal Emulgel

Surini

Leonyza

Suh³

2020

Adv Pharm Bull

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Purpose : Recombinant human epidermal growth factor (rhEGF) is a 6045-Da peptide that promotes the cell growth process, and it is also used for cosmetic purposes as an anti-aging compound. However, its penetration into skin is limited by its large molecular size. This study aimed to prepare rhEGF-loaded transfersomal emulgel with enhanced skin penetration compared with that of non-transfersomal rhEGF emulgel. Methods: Three transfersome formulations were prepared with different ratios between the lipid vesicle (phospholipid and surfactant) and rhEGF (200:1, 133:1, and 100:1) using a thin-film hydration-extrusion method. The physicochemical properties of these transfersomes and the percutaneous delivery of the transfersomal emulgel were evaluated. Long-term and accelerated stability studies were also conducted. Results: The 200:1 ratio of lipid to drug was optimal for rhEGF-loaded transfersomes, which had a particle size of 128.1 ± 0.66 nm, polydispersity index of 0.109 ± 0.004, zeta potential of −43.1 ± 1.07 mV, deformability index of 1.254 ± 0.02, and entrapment efficiency of 97.77% ± 0.09%. Transmission electron microscopy revealed that the transfersomes had spherical and unilamellar vesicles. The skin penetration of rhEGF was enhanced by as much as 5.56 fold by transfersomal emulgel compared with that of non-transfersomal emulgel. The stability study illustrated that the rhEGF levels after 3 months were 84.96–105.73 and 54.45%–66.13% at storage conditions of 2°C–8°C and 25°C ± 2°C/RH 60% ± 5%, respectively. Conclusion: The emulgel preparation containing transfersomes enhanced rhEGF penetration into the skin, and skin penetration was improved by increasing the lipid content.

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Section: Deformability Indexmentioning

confidence: 99%

“…Because the pH was slightly higher than the isoelectric point of phosphatidylcholine, phosphatidylcholine carried a negative charge. 21,31 Moreover, the edge activator (sodium deoxycholate) is an anionic surfactant that also contributed the net negative charge of the formulations. 26…”

Section: Particle Size and Zeta Potentialmentioning

confidence: 99%

Formulation and In Vitro Penetration Study of Recombinant Human Epidermal Growth Factor-Loaded Transfersomal Emulgel

Surini

Leonyza

Suh³

2020

Adv Pharm Bull

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“…Phosphatidylcholine is a zwitterionic compound with an isoelectric point between 6 and 7. During the process of formulation, pH 7.2 phosphate buffer solution was used as the hydrating medium, where the pH was a little bit higher than the isoelectric point of phosphatidylcholine, causing phosphatidylcholine carried a negative charge [23,24]. Moreover, sodium deoxycholate used is an anionic surfactant, which also contributed to the net negative charge of the formulation [13].…”

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confidence: 99%

Optimization of Sodium Deoxycholate-Based Transfersomes for Percutaneous Delivery of Peptides and Proteins

Leonyza

Surini

2019

Int J App Pharm

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Objective: This study aims to formulate and characterize the transfersomes for percutaneous delivery of peptides and proteins. In particular, this study was a preliminary study for the transfersomes formulation of recombinant human epidermal growth factor (rhEGF) for topical delivery. Methods: The transfersomes was prepared by thin film hydration method using phosphatidylcholine and sodium deoxycholate as vesicle former. In this study, transfersomes formulas were optimized, namely TF1, TF2, TF3, and TF4 with several ratios of phospholipid and surfactant which were 90:10, 85:15, 80:20, and 75:25, respectively. Afterward, the transfersomes were characterized in terms of particle size distribution, polydispersity index, zeta potential, morphology of vesicles, and deformability index. Results: The results showed that the best formulation was TF3 with the ratio of 80:20 with a particle size of 118.6±1.33 nm, polydispersity index of 0.102±0.011, zeta potential of-30.9±0,46 mV, and deformability index of 1.182±0.08. TEM analysis also showed spherical and unilamellar vesicles of transfersomes. Conclusion: This work demonstrated that the sodium deoxycholate-based transfersomes could be potential to be further formulated with peptide and protein for percutaneous delivery.

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“…This finding can be due to the low stability of niosomes after manufacture, as shown by the zeta potential value. A sample is called 'stable' if it has a zeta potential more positive than +30 mV or more negative than -30 mV (Surini et al, 2018). The zeta potential of the green tea extract niosomes sample was -0.616 mV (Table 3), which means that the green tea extract niosomes preparation is less stable than found in a previous study in which it was reported as -39.9 mV (Isnan & Jufri, 2017).…”

Section: Real-time Stability Testmentioning

confidence: 80%

Thin-layer hydration method to prepare a green tea extract niosomal gel and its antioxidant performance

Chasanah

Mahmintari

Hidayah

et al. 2021

European Pharmaceutical Journal

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This study aimed to prepare a niosomal gel of green tea (Camellia sinensis) extract containing catechins, mostly epigallocatechin-3-gallate (ECGC), as a potent antioxidant. Niosomes can increase EGCG's stability and penetration into the skin for a better therapeutic effect. Niosomes were prepared by a thin-layer hydration method, were evaluated for their vesicle shape, particle size, polydispersity index, zeta potential and entrapment efficiency, and then incorporated into gels using sodium alginate as a gelling agent. Three niosomal gel formulations were prepared with different concentrations of niosomes green tea extract. Afterwards, organoleptic properties, chemical and physical characteristics, antioxidant activity, and stability and irritability of the niosomal gels were investigated. The different concentrations of green tea extract had a significant effect on the physical characteristics, but not on the chemical ones. Its antioxidant activity was determined using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging method. The 50% extract green tea niosomes gel showed the highest inhibition value (25.13%). The stability was determined by freeze–thaw and real-time methods; they showed a decrease in pH, but still within the pH range of skin. The irritability test used was the Hen's Egg Test-Chorioallantoic Membrane (HET-CAM) method, which showed no irritation for all formulas. In conclusion, 50% green tea extract niosomes gel results showed it to be the best formulation with optimal antioxidant results.

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Formulation and in vitro Penetration Study of Transfersomes Gel Containing Gotu Kola Leaves Extract (Centella asiatica L. Urban)

Cited by 19 publications

References 11 publications

Formulation and In Vitro Penetration Study of Recombinant Human Epidermal Growth Factor-Loaded Transfersomal Emulgel

Formulation and In Vitro Penetration Study of Recombinant Human Epidermal Growth Factor-Loaded Transfersomal Emulgel

Optimization of Sodium Deoxycholate-Based Transfersomes for Percutaneous Delivery of Peptides and Proteins

Thin-layer hydration method to prepare a green tea extract niosomal gel and its antioxidant performance

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