Formulation, Characterization and Evaluation of a Thermoresponsive &lt;I&gt;In&lt;/I&gt; &lt;I&gt;Situ&lt;/I&gt; Gel Containing Gallic Acid-Loaded Chitosan Nanoparticles for the Treatment of Periodontal Disease

Patil, Ashwini; Jobanputra, Arpana H.

doi:10.1166/jbns.2015.1324

Cited by 2 publications

(2 citation statements)

References 26 publications

(29 reference statements)

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“…Three milliliter aliquots were removed at predetermined times (0.25, 0.5, 1, 2, 4, 6, 8, and 24 h) and compensated with the same volume of the release medium. 40 Finally, the concentration of GA was determined via spectrophotometry at λ max of 258 nm. 40 , 41 The cumulative percentage of GA released was calculated using Equation 3.…”

Section: Methodsmentioning

confidence: 99%

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<p>Zein/Phospholipid Composite Nanoparticles for Successful Delivery of Gallic Acid into aHSCs: Influence of Size, Surface Charge, and Vitamin A Coupling</p>

Radwan¹,

El-Maadawy²,

Yousry³

et al. 2020

IJN

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Purpose Zein/phospholipid composite nanoparticles (CNPs) were developed as a delivery platform for gallic acid (GA), a polyphenolic compound with reported preclinical antifibrotic activities. However, the therapeutic applicability of GA is hampered owing to its low bioavailability and rapid clearance. Accordingly, we developed GA-loaded CNPs. The effect of their size, surface charge and targeting strategies was investigated and optimized, with the aim of enhancing their ability to deliver GA to the activated hepatic stellate cells (aHSCs) in order to suppress hepatic fibrosis progression. Methods Different CNP systems were prepared and characterized with regard to their particle size, zeta potential, and GA entrapment efficiency (EE%). Also, they were statistically optimized via response surface methodology. The optimized systems were investigated with regard to their in vitro GA release, in vitro efficacy on aHSCs, and in vivo biodistribution in healthy rats. Results The GA-loaded cationic CNPs coupled with vitamin A (GA-CACNP/VA; 192 nm) showed high GA EE% (60% w/w), highest cellular internalization via active targeting, and more selective hepatic distribution, relative to free GA solution, GA-loaded anionic, and GA-loaded cationic systems. Furthermore, GA-CACNP/VA markedly triggered the apoptosis of aHSCs, repressed collagen deposition, and inhibited HSCs’ activation to a lesser extent. Conclusion The GA-CACNP/VA was shown to be a promising candidate for specific and controlled delivery of GA to aHSCs, which may provide an effective antifibrotic therapeutic approach.

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Section: Methodsmentioning

confidence: 99%

“…40 Finally, the concentration of GA was determined via spectrophotometry at λ max of 258 nm. 40,41 The cumulative percentage of GA released was calculated using Equation 3. 42 Cumulative percentage of GA released…”

Section: In Vitro Drug Release Studymentioning

confidence: 99%