Fos-like expression and nuclear size in osmotically stimulated supraoptic nucleus neurons

Lafarga, Miguel; Berciano, Marı́a T.; Martínez-Guijarro, Francisco-José; Andrés, M. A.; Mellström, Britt; López‐García, Carlos; Naranjo, José R.

doi:10.1016/0306-4522(92)90210-s

Cited by 52 publications

(23 citation statements)

References 51 publications

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“…2). These structures have some resemblance to the indentations of the nuclear membranes and grooves observed in several cell types in the primate and rodent hippocampus (Ribak and Seress, 1983;Frotscher et al, 1988;Soriano et al, 1990), onion epidermal cells and tobacco culture cells (Collings et al, 2000), and also to alterations in nuclear architecture found following stress, injury, or aging (Honavar and Lantos, 1987;Lafarga et al, 1992;Borsello et al, 2002;Dorsey et al, 2006). However, they appear morphologically distinct from the tube-like structures found in HeLa cells and various other non-neuronal cell lines (Fricker et al, 1997) and the highly lobulated nuclei found in fibroblasts from patients with Hutchinson-Gilford Progeria Syndrome (Goldman et al, 2004).…”

Section: Morphological Analysis and 3d Image Reconstruction Of Hippocmentioning

confidence: 63%

Synaptic Activity Induces Dramatic Changes in the Geometry of the Cell Nucleus: Interplay between Nuclear Structure, Histone H3 Phosphorylation, and Nuclear Calcium Signaling

Wittmann

Queisser²,

Eder³

et al. 2009

J. Neurosci.

122

159

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Synaptic activity initiates many adaptive responses in neurons. Here we report a novel form of structural plasticity in dissociated hippocampal cultures and slice preparations. Using a recently developed algorithm for three-dimensional image reconstruction and quantitative measurements of cell organelles, we found that many nuclei from hippocampal neurons are highly infolded and form unequally sized nuclear compartments. Nuclear infoldings are dynamic structures, which can radically transform the geometry of the nucleus in response to neuronal activity. Action potential bursting causing synaptic NMDA receptor activation dramatically increases the number of infolded nuclei via a process that requires the ERK-MAP kinase pathway and new protein synthesis. In contrast, deathsignaling pathways triggered by extrasynaptic NMDA receptors cause a rapid loss of nuclear infoldings. Compared with near-spherical nuclei, infolded nuclei have a larger surface and increased nuclear pore complex immunoreactivity. Nuclear calcium signals evoked by cytosolic calcium transients are larger in small nuclear compartments than in the large compartments of the same nucleus; moreover, small compartments are more efficient in temporally resolving calcium signals induced by trains of action potentials in the theta frequency range (5 Hz). Synaptic activity-induced phosphorylation of histone H3 on serine 10 was more robust in neurons with infolded nuclei compared with neurons with near-spherical nuclei, suggesting a functional link between nuclear geometry and transcriptional regulation. The translation of synaptic activity-induced signaling events into changes in nuclear geometry facilitates the relay of calcium signals to the nucleus, may lead to the formation of nuclear signaling microdomains, and could enhance signal-regulated transcription.

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Section: Morphological Analysis and 3d Image Reconstruction Of Hippocmentioning

confidence: 63%

Synaptic Activity Induces Dramatic Changes in the Geometry of the Cell Nucleus: Interplay between Nuclear Structure, Histone H3 Phosphorylation, and Nuclear Calcium Signaling

Wittmann

Queisser²,

Eder³

et al. 2009

J. Neurosci.

122

159

View full text Add to dashboard Cite

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“…Dehydration by salt loading induced rapid c-fos expression (detected by in situ hybridization) in the PVN, reaching a peak at 30 min after the stimulus (Ding et al,1994). Other investigators, using immunocytochemistry, reported Fos-positive cells in both the SON and PVN 30 min after salt loading (Giovannelli and Bloom, 1992) and a profound increase in the SON after 1 or 6 days of salt loading (Lafarga et al, 1992). Our analysis by northern blot hybridization reveals increased c-fos mRNA levels 6 h after water deprivation in both hypothalamic nuclei.…”

Section: Discussionmentioning

confidence: 93%

Time Course of c-fos, vasopressin and oxytocin mRNA Expression in the Hypothalamus Following Long-Term Dehydration

Silveira

Junta²,

Monesi

et al. 2007

Cell Mol Neurobiol

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1. This study presents a time course analysis of the messenger RNA (mRNA) levels of c-fos, vasopressin (VP), and oxytocin (OT) in the paraventricular (PVN) and supraoptic nucleus (SON), following acute and chronic dehydration by water deprivation. 2. Male Wistar rats were separated into five groups: nondehydrated (control group) and dehydrated for 6, 24, 48 and 72 h. Following water deprivation, animals were decapitated, their blood was collected for hematocrit, osmolality, and plasma sodium measurements, and brains were removed for dissection of both PVN and SON. 3. As expected, the hematocrit, osmolality, plasma sodium, and weight loss were increased after water deprivation. In SON, a significant increase in both VP and OT mRNA expression was observed 6 h after dehydration reaching a peak at 24 h and returning to basal levels of expression at 72 h. In the PVN, an increase in both VP and OTmRNA expression occurred 24 h after dehydration. At 72 h the VP and OT mRNA expression levels had decreased but they were still at higher levels than those detected in control animals. 4. These results suggest that SON is the first nucleus to respond to the dehydration stimulus. Additionally, we also observed an increase in c-fos mRNA expression in both PVN and SON 6 h after water deprivation, which progressively decreased 24, 48, and 72 h after the onset of water deprivation. Therefore, it is possible that c-fos may be involved in the modulation of VP and OT genes, regulating the mRNA expression levels on a temporally distinct basis within the PVN and SON.

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“…This increase in IGF-I-R mRNA was accompanied by high intensity c-Fos immunostaining in the same cells. Several studies have shown that c-Fos expression is increased in activated neurones, including those of the hypo¬ thalamus (Sagar et al 1988;Lafarga et al 1992). In the present study, we utilized double labelling with immunocytochemical staining for c-Fos and in-situ hybridization for IGF-I-R mRNA to identify those hypothalamic neurones activated by systemic dehydration that also expressed the IGF-I-R gene.…”

Section: Resultsmentioning

confidence: 99%

Distribution of insulin-like growth factor-I receptor mRNA in rat brain. Regulation in the hypothalamo-neurohypophysial system

Aguado

Rodrigo²,

Cacicedo³

et al. 1993

Journal of Molecular Endocrinology

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The distribution and regulation of mRNA for the IGF-I receptor (IGF-I-R) in the adult rat brain were studied by in-situ hybridization with a 35S-labelled cRNA probe. The pituitary gland showed a strong hybridization signal in the pars tuberalis (the surface of the median eminence), pars distalis and pars intermedia. Within the brain, a strong hybridization signal was found in the circumventricular organs, olfactory bulb, hippocampus, cerebellum and hypothalamus. IGF-I-R mRNA was consistently found in cell bodies of the hypothalamo-neurohypophysial systern. Six days of intermittent salt-loading resulted in an increase in IGF-I-R gene expression in the supraoptic nucleus. The increase in IGF-I-R mRNA was accompanied by a high expression of c-Fos immunoreactivity in the same cells. The presence and regulation of IGF-I-R mRNA in the hypothalamus suggest that IGF-I may affect the local plasticity or modulation of activated magnocellular neurones by an autocrine or paracrine action through specific receptors in the hypothalamo-neurohypophysial system.

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Fos-like expression and nuclear size in osmotically stimulated supraoptic nucleus neurons

Cited by 52 publications

References 51 publications

Synaptic Activity Induces Dramatic Changes in the Geometry of the Cell Nucleus: Interplay between Nuclear Structure, Histone H3 Phosphorylation, and Nuclear Calcium Signaling

Synaptic Activity Induces Dramatic Changes in the Geometry of the Cell Nucleus: Interplay between Nuclear Structure, Histone H3 Phosphorylation, and Nuclear Calcium Signaling

Time Course of c-fos, vasopressin and oxytocin mRNA Expression in the Hypothalamus Following Long-Term Dehydration

Distribution of insulin-like growth factor-I receptor mRNA in rat brain. Regulation in the hypothalamo-neurohypophysial system

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