Fossil eggshells of amniotes as a paleothermometry tool

Choi, Seung Hyun; Park, Yong; Kweon, Jin Jung; Kim, Seongyeong; Jung, Haemyeong; Lee, Sung Keun; Lee, Yuong‐Nam

doi:10.1016/j.palaeo.2021.110376

“…[ 28 ] match their corresponding Raman signatures, as do those over the entire spectral range (Figure 4A–F). Our ATR FT‐IR spectra also resemble published FT‐IR spectra [ 60–62 ] for comparable samples [ 61,62 ] and are reproducible over different regions of carbonaceous fossils (Figure 4E). Thus the new ATR FT‐IR analyses confirm the validity of our Raman spectra.…”

Section: Fourier‐transform Infrared Spectroscopy Independently Valida...supporting

confidence: 79%

“…ATR FT-IR signals in the organic fingerprint region (analyzed by means of multivariate statistics) of representative specimens from the studies disputed by Alleon et al [28] match their corresponding Raman signatures, as do those over the entire spectral range (Figure 4A-F). Our ATR FT-IR spectra also resemble published FT-IR spectra [60][61][62] for comparable samples [61,62] and are reproducible over different regions of carbonaceous fossils (Figure 4E). Thus the new ATR FT-IR analyses confirm the validity of our Raman spectra.…”

Section: Fourier-transform Infrared Spectroscopy Independently Valida...supporting

confidence: 77%

“…(A) Modern Furcifer bone, (B) fossil Allosaurus bone, [3] (C) modern Dromaius eggshell, [5] (D) fossil Heyuannia eggshell, [4] (E) fossil Mazon Creek Acanthotelson carbonaceous remains, FT-IR spectra were collected at two different positions of the carbonaceous fossil, [12] (F) fossil Burgess Shale Marrella carbonaceous film. [11] Referenced Raman spectra [14,21,22] (light blue lines) and FT-IR spectra [60][61][62] (light red lines) from the literature are included. The ChemoSpace plot in Figure 3I combines all the disputed samples and therefore incorporates all the loadings contributing to the separation of clusters in Figure 3A-H.…”

Section: Our Raman Spectra Do Not Contain Edge Filter Ripplesmentioning

confidence: 99%

See 1 more Smart Citation

Raman spectroscopy is a powerful tool in molecular paleobiology: An analytical response to Alleon et al. (https://doi.org/10.1002/bies.202000295)

Wiemann

¹

,

Briggs

²

2022

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A recent article argued that signals from conventional Raman spectroscopy of organic materials are overwhelmed by edge filter and fluorescence artefacts. The article targeted a subset of Raman spectroscopic investigations of fossil and modern organisms and has implications for the utility of conventional Raman spectroscopy in comparative tissue analytics. The inferences were based on circular reasoning centered around the unconventional analysis of spectra from just two samples, one modern, and one fossil. We validated the disputed signals with in situ Fourier‐Transform Infrared (FT‐IR) Spectroscopy and through replication with different lasers, filters, and operators in independent laboratories. Our Raman system employs a holographic notch filter which is not affected by edge filter or other artefacts. Multiple lines of evidence confirm that conventional Raman spectra of fossils contain biologically and geologically meaningful information. Statistical analyses of large Raman and FT‐IR spectral data sets reveal patterns in fossil composition and yield valuable insights into the history of life.

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“…The prominent slash patterns inside the SqZ are calcite twinning, which are diagenetically deformed calcite structure only found in fossil eggshells (i.e. abiogenic in origin; Choi et al, 2021 ). See also Grellet-Tinner and Dyke, 2005 for SEM micrographs of Lithornis vulturinus eggshell that has wedge-like ML.…”

Section: Resultsmentioning

confidence: 99%

Microstructural and crystallographic evolution of palaeognath (Aves) eggshells

Choi

¹

,

Hauber

²

,

Legendre

³

et al. 2023

eLife

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The avian palaeognath phylogeny has been recently revised significantly due to the advancement of genome-wide comparative analyses and provides the opportunity to trace the evolution of the microstructure and crystallography of modern dinosaur eggshells. Here, eggshells of all major clades of Palaeognathae (including extinct taxa) and selected eggshells of Neognathae and non-avian dinosaurs are analysed with electron backscatter diffraction. Our results show the detailed microstructures and crystallographies of (previously) loosely categorized ostrich-, rhea-, and tinamou-style morphotypes of palaeognath eggshells. All rhea-style eggshell appears homologous, while respective ostrich-style and tinamou-style morphotypes are best interpreted as homoplastic morphologies (independently acquired). Ancestral state reconstruction and parsimony analysis additionally show that rhea-style eggshell represents the ancestral state of palaeognath eggshells both in microstructure and crystallography. The ornithological and palaeontological implications of the current study are not only helpful for the understanding of evolution of modern and extinct dinosaur eggshells, but also aid other disciplines where palaeognath eggshells provide useful archive for comparative contrasts (e.g. palaeoenvironmental reconstructions, geochronology, and zooarchaeology).

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“…The prominent slash patterns inside the SqZ are calcite twinning, which are diagenetically deformed calcite structure only found in fossil eggshells (i.e. abiogenic in origin; Choi et al, 2021, under review). See also Grellet-Tinner and Dyke (2005) for SEM micrographs of Lithornis vulturinus eggshell that has wedge-like ML.…”

Section: Resultsmentioning

confidence: 99%

Microstructural and crystallographic evolution of palaeognath (Aves) eggshells

Choi

¹

,

Hauber

²

,

Legendre

³

et al. 2022

Preprint

Self Cite

0

View full text Add to dashboard Cite

The avian palaeognath phylogeny has been recently revised significantly due to the advancement of genome-wide comparative analyses and provides the opportunity to trace the evolution of the microstructure and crystallography of modern dinosaur eggshells. Here, eggshells of all major clades of Palaeognathae (including extinct taxa) and selected eggshells of Neognathae and non-avian dinosaurs are analysed with electron backscatter diffraction. Our results show the detailed microstructures and crystallographies of (previously) loosely categorized ostrich-, rhea-, and tinamou-style morphotypes of palaeognath eggshells. All rhea-style eggshell appears homologous, while respective ostrich-style and tinamou-style morphotypes are best interpreted as homoplastic morphologies (independently acquired). Ancestral state reconstruction and parsimony analysis additionally show that rhea-style eggshell represents the ancestral state of palaeognath eggshells both in microstructure and crystallography. The ornithological and palaeontological implications of the current study are not only helpful for the understanding of evolution of modern and extinct dinosaur eggshells, but also aid other disciplines where palaeognath eggshells provide useful archive for comparative contrasts (e.g. palaeoenvironmental reconstructions, geochronology, and zooarchaeology).

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Fossil eggshells of amniotes as a paleothermometry tool

Cited by 13 publications

References 151 publications

Raman spectroscopy is a powerful tool in molecular paleobiology: An analytical response to Alleon et al. (https://doi.org/10.1002/bies.202000295)

Raman spectroscopy is a powerful tool in molecular paleobiology: An analytical response to Alleon et al. (https://doi.org/10.1002/bies.202000295)

Microstructural and crystallographic evolution of palaeognath (Aves) eggshells

Microstructural and crystallographic evolution of palaeognath (Aves) eggshells

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