Fostering efficacy and toxicity evaluation of traditional Chinese medicine and natural products: Chick embryo as a high throughput model bridging in vitro and in vivo studies

Wu, Tong; Yu, Guiyuan; Xiao, Jia; Yan, Chunri; Kojima, Hiroshi; Li, Yi-Fang; So, Kwok-Fai; He, Rong‐Rong

doi:10.1016/j.phrs.2018.04.011

Cited by 20 publications

(9 citation statements)

References 127 publications

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“…The unknown toxicity of TCM has always been an important factor restricting its clinical application [ 16 ]. Although PLB presents broad pharmacological activity, the obvious toxicities of PLB are not negligible.…”

Section: Discussionmentioning

confidence: 99%

Plumbagin can potently enhance the activity of xanthine oxidase: in vitro, in vivo and in silico studies

Yue

Jiang

et al. 2021

BMC Pharmacol Toxicol

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Background Abnormally elevated xanthine oxidase (XO) activity has been verified to cause various pathological processes, such as gout, oxidative stress injury and metabolic syndrome. Thus, XO activators may exhibit above potential toxicological properties. Plumbagin (PLB) is an important active compound in traditional Chinese medicine (TCM), while its obvious toxic effects have been reported, including diarrhea, skin rashes and hepatic toxicity. However, the potential toxicity associated with enhancement of XO activity has not been fully illuminated so far. Methods The present study investigated the effect of PLB on XO activity by culturing mouse liver S9 (MLS9), human liver S9 (HLS9), XO monoenzyme system with PLB and xanthine. Then, the molecular docking and biolayer interferometry analysis were adopted to study the binding properties between PLB and XO. Finally, the in vivo acceleration effect also investigated by injected intraperitoneally PLB to KM mice for 3 days. Results PLB could obviously accelerate xanthine oxidation in the above three incubation systems. Both the Vmax values and intrinsic clearance values (CLint, Vmax/Km) of XO in the three incubation systems increased along with elevated PLB concentration. In addition, the molecular docking study and label-free biolayer interferometry assay displayed that PLB was well bound to XO. In addition, the in vivo results showed that PLB (2 and 10 mg/kg) significantly increased serum uric acid levels and enhanced serum XO activity in mice. Conclusion In summary, this study outlines a potential source of toxicity for PLB due to the powerful enhancement of XO activity, which may provide the crucial reminding for the PLB-containing preparation development and clinical application.

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Section: Discussionmentioning

confidence: 99%

Plumbagin can potently enhance the activity of xanthine oxidase: in vitro, in vivo and in silico studies

Yue

Jiang

et al. 2021

BMC Pharmacol Toxicol

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“…Without available treatment to restore permanent salivary flow, tissue engineers want to produce implantable miniature salivary secretory units [ 3 , 4 , 5 , 6 , 7 ]. Furthermore, another benefit of engineering SGs—or tissues in general—are their implementation as in vitro models that are usable in preclinical studies [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

“…More recently, studies have incorporated human embryonic stem cells into developing chick embryos [ 44 , 45 ] that matured into developing xenograft mammalian tissues. Scientists have also used the entire developing egg and its chorioallaentoic membrane (a vascular membrane found in eggs) as an angiogenic preclinical drug-testing device [ 9 , 46 ]. Recently, the usage of egg-derived biomaterials as a 3D gel (especially the EW [ 47 , 48 ]) and most recently in EY and its fractions [ 49 ] have been investigated.…”

Section: Introductionmentioning

confidence: 99%

3D Cell Culture of Human Salivary Glands Using Nature-Inspired Functional Biomaterials: The Egg Yolk Plasma and Egg White

Charbonneau

Tran

2020

Materials

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The egg yolk plasma (EYP)—a translucent fraction of the egg yolk (EY) obtained by centrifugation—was tested as a developmentally encouraging, cost-effective, biomaterial for salivary gland (SG) tissue engineering. To find optimal incubating conditions for both the human NS-SV-AC SG acinar cell line and SG fibroblasts, cells were stained with Live/Dead®. The cellular contents of 96-well plates were analyzed by high content screening image analysis. Characteristically, the EYP biomaterial had lipid and protein content resembling the EY. On its own, the EYP was non-conducive to cell survival. EYP’s pH of 6 mainly contributed to cell death. This was demonstrated by titrating EYP’s pH with different concentrations of either commercial cell culture media, NaOH, or egg white (EW). These additives improved SG mesenchymal and epithelial cell survival. The best combinations were EYP diluted with (1) 70% commercial medium, (2) 0.02 M NaOH, or (3) 50% EW. Importantly, commercial medium-free growth was obtained with EYP + NaOH or EYP + EW. Furthermore, 3D cultures were obtained as a result of EW’s gelatinous properties. Here, the isolation, characterization, and optimization of three EYP-based biomaterial combinations are shown; two were free of commercial medium or supplements and supported both SG cells’ survival.

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“…Human cells have been added to developing fertilized chick embryo [17,18], egg white (EW) [19,20], and EY [21,22,23]. Additionally, pharmacological studies use the egg environment for drug screening [24]. In another of our reports on egg biomaterials [Submitted], we also exposed two salivary cell types to EYP + Media or EYP + EW.…”

Section: Introductionmentioning

confidence: 99%

3D Cultures of Salivary Gland Cells in Native or Gelled Egg Yolk Plasma, Combined with Egg White and 3D-Printing of Gelled Egg Yolk Plasma

2019

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For salivary gland (SG) tissue engineering, we cultured acinar NS-SV-AC cell line or primary SG fibroblasts for 14 days in avian egg yolk plasma (EYP). Media or egg white (EW) supplemented the cultures as they grew in 3D-Cryo histology well inserts. In the second half of this manuscript, we measured EYP’s freeze-thaw gelation and freeze-thaw induced gelled EYP (GEYP), and designed and tested further GEYP tissue engineering applications. With a 3D-Cryo well insert, we tested GEYP as a structural support for 3D cell culture or as a bio-ink for 3D-Bioprinting fluorescent cells. In non-printed EYP + EW or GEYP + EW cultures, sagittal sections of the cultures showed cells remaining above the well’s base. Ki-67 expression was lacking for fibroblasts, contrasting NS-SV-AC’s constant expression. Rheological viscoelastic measurements of GEYP at 37 °C on seven different freezing periods showed constant increase from 0 in mean storage and loss moduli, to 320 Pa and 120 Pa, respectively, after 30 days. We successfully 3D-printed GEYP with controlled geometries. We manually extruded GEYP bio-ink with fluorescence cells into a 3D-Cryo well insert and showed cell positioning. The 3D-Cryo well inserts reveal information on cells in EYP and we demonstrated GEYP cell culture and 3D-printing applications.

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Fostering efficacy and toxicity evaluation of traditional Chinese medicine and natural products: Chick embryo as a high throughput model bridging in vitro and in vivo studies

Cited by 20 publications

References 127 publications

Plumbagin can potently enhance the activity of xanthine oxidase: in vitro, in vivo and in silico studies

Plumbagin can potently enhance the activity of xanthine oxidase: in vitro, in vivo and in silico studies

3D Cell Culture of Human Salivary Glands Using Nature-Inspired Functional Biomaterials: The Egg Yolk Plasma and Egg White

3D Cultures of Salivary Gland Cells in Native or Gelled Egg Yolk Plasma, Combined with Egg White and 3D-Printing of Gelled Egg Yolk Plasma

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