Four-dimensional X-ray phase tomography with Talbot interferometry and white synchrotron radiation: dynamic observation of a living worm

Momose, Atsushi; Yashiro, Wataru; Harasse, Sébastien; Kuwabara, Hiroaki

doi:10.1364/oe.19.008423

Cited by 103 publications

(65 citation statements)

References 26 publications

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“…A 'single shot' Fourier transform method is useful for a fast measurement since it is easy to control the Moiré fringes. Four-dimensional X-ray phase contrast tomography has been demonstrated using this method and white synchrotron radiation (Momose et al, 2011). However, the spatial resolution of the phase image is restricted by the period of the carrier fringes.…”

Section: High-throughput X-ray Phase Contrast Tomographymentioning

confidence: 99%

Quantitative and dynamic measurements of biological fresh samples with X-ray phase contrast tomography

Hoshino

Uesugi

Tsukube

et al. 2014

J Synchrotron Radiat

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X-ray phase contrast tomography using a Talbot grating interferometer was applied to biological fresh samples which were not fixed by any fixatives. To achieve a high-throughput measurement for the fresh samples the X-ray phase contrast tomography measurement procedure was improved. The threedimensional structure of a fresh mouse fetus was clearly depicted as a mass density map using X-ray phase contrast tomography. The mouse fetus measured in the fresh state was then fixed by formalin and measured in the fixed state. The influence of the formalin fixation on soft tissue was quantitatively evaluated by comparing the fresh and fixed samples. X-ray phase contrast tomography was also applied to the dynamic measurement of a biological fresh sample. Morphological changes of a ring-shaped fresh pig aorta were measured tomographically under different degrees of stretching.

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Section: High-throughput X-ray Phase Contrast Tomographymentioning

confidence: 99%

Quantitative and dynamic measurements of biological fresh samples with X-ray phase contrast tomography

Hoshino

Uesugi

Tsukube

et al. 2014

J Synchrotron Radiat

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“…Combining a micro-CT approach in the Talbot interferometer with polychromatic SR enables time-resolved X-ray phase tomography, which is expected to provide precise morphological and functional information under static conditions. 97 However, the need to acquire multiple images per projections with interferometry makes it unlikely that these approaches can be applied to dynamic organs, such as the heart or lungs.…”

Section: Other Sr Techniques and Simultaneous Multi-sr Imaging Approamentioning

confidence: 99%

Synchrotron Radiation Imaging for Advancing Our Understanding of Cardiovascular Function

Shirai

Schwenke

Tsuchimochi

et al. 2013

Circulation Research

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Synchrotron radiation (SR) is increasingly being used for micro-level and nano-level functionalimaging in in vivo animal experiments. This review focuses on the methodology that enables repeated and regional assessment of vessel internal diameter and flow in the resistance vessels of different organ systems. In particular, SR absorption microangiography approaches offer unique opportunities for real-time in vivo vascular imaging in small animals, even during dynamic motion of the heart and lungs. We also describe recent progress in the translation of multiple phase-contrast imaging techniques from ex vivo to in vivo smallanimal studies. Furthermore, we also review the utility of SR for multiple pinpoint (dimensions 0.2×0.2 mm) assessments of myocardial function at the cross-bridge level in different regions of the heart using small-angle X-ray scattering, resulting from increases in SR flux at modern facilities. Finally, we present cases for the use of complementary SR approaches to study cardiovascular function, particularly the pathological changes associated with disease using small-animal models. -times brighter than laboratory or medical X-ray sources). This brightness is attributable to the fine collimation of the X-ray beam (http:// www.spring8.or.jp/en/about_us/whats_sr/generation_sr/).At SPring-8 in Japan, which is one of the largest SR facilities in the world, one of the smallest beam sizes is ≈2 mm horizontally by 0.4 mm vertically at 40 m from the X-ray source. This divergence is much smaller than that of most laboratory lasers. The X-ray beam is further collimated with focusing optics, so that it is <0.2 mm in diameter at the sample, concentrating a large number of X-ray photons on the sample. There are tens of SR facilities around the world, most of which are available for public access (for details on SR facilities, SR characteristics, and other science applications, http://www.lightsources.org/cms/).The intense X-ray beam from the synchrotron beamlines only can be used in metal-shielded hutches. X-ray image formation and acquisition have to be performed under remote control, including operations to inject contrast medium into an animal or to stop artificial ventilation temporarily. Despite this restriction, when the animal is closely monitored, the experiment can be performed smoothly. At SR medical imaging beamlines important infrastructure for animal experiments such as an animal house, and biology and chemistry preparation laboratories are an essential capability for the experiments described in this review. SR Absorption MicroangiographyThe vascular smooth muscle tone of arterioles is meticulously modulated via multiple mechanistic pathways, including, but not limited to, the vessel endothelium, the vascular smooth muscle, and neurohumoral stimuli to regulate resistance to flow and, hence, to optimize regional blood flow. Impairment of any of these regulatory systems has the potential to adversely impact on the vascular control of blood flow and, thus, impair the homeostatic control...

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“…From its first implementations up to this day, grating interferometry has been mainly exploited for imaging biological soft-tissue samples (8,9,16,17,21). However, other classes of samples, such as paleontological specimens, can also benefit from the high sensitivity of the phase and darkfield signals provided by this technique.…”

Section: Resultsmentioning

confidence: 99%

“…Among these methods, the "phase-stepping" technique, which consists of recording and analyzing a series of interferograms (three or more) for different relative transverse positions of the two gratings (7,11,14), has several advantages. It does not affect the spatial resolution of the imaging system as in single-shot approaches (15,16) and it does not have stringent requirements on the period matching of the gratings, which is a limitation in the reverse projections (RP) method (17). It should also be noted that, with the RP method, which uses a linear combination of two interferograms taken at sample positions 180°a part, the dark-field signal cannot be accessed.…”

mentioning

confidence: 99%

Trimodal low-dose X-ray tomography

Zanette

Bech

Rack

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

111

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X-ray grating interferometry is a coherent imaging technique that bears tremendous potential for three-dimensional tomographic imaging of soft biological tissue and other specimens whose details exhibit very weak absorption contrast. It is intrinsically trimodal, delivering phase contrast, absorption contrast, and scattering (“dark-field”) contrast. Recently reported acquisition strategies for grating-interferometric phase tomography constitute a major improvement of dose efficiency and speed. In particular, some of these techniques eliminate the need for scanning of one of the gratings (“phase stepping”). This advantage, however, comes at the cost of other limitations. These can be a loss in spatial resolution, or the inability to fully separate the three imaging modalities. In the present paper we report a data acquisition and processing method that optimizes dose efficiency but does not share the main limitations of other recently reported methods. Although our method still relies on phase stepping, it effectively uses only down to a single detector frame per projection angle and yields images corresponding to all three contrast modalities. In particular, this means that dark-field imaging remains accessible. The method is also compliant with data acquisition over an angular range of only 180° and with a continuous rotation of the specimen.

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Four-dimensional X-ray phase tomography with Talbot interferometry and white synchrotron radiation: dynamic observation of a living worm

Cited by 103 publications

References 26 publications

Quantitative and dynamic measurements of biological fresh samples with X-ray phase contrast tomography

Quantitative and dynamic measurements of biological fresh samples with X-ray phase contrast tomography

Synchrotron Radiation Imaging for Advancing Our Understanding of Cardiovascular Function

Trimodal low-dose X-ray tomography

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