Four DNA-A variants among Pakistani isolates of cotton leaf curl virus and their affinities to DNA-A of geminivirus isolates from okra.

Zhou, Xueping; Liu, Yule; Robinson, David J.; Harrison, B. D.

doi:10.1099/0022-1317-79-4-915

Cited by 159 publications

(126 citation statements)

References 15 publications

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“…As previously reported for several begomoviruses (Zhou et al, 1998), V1 and C3 ORFs of AGV overlap in 4 nt, including their respective stop codons. Pair-wise comparisons between AGV and closely related geminiviruses, including citrus chlorotic dwarf-associated virus, grapevine red-blotch-associated virus and Jatropha mosaic virus, which are geminiviruses infecting woody hosts, showed nucleotide sequence identity of *56 % when the full genomes were considered (Table 1).…”

Section: Genome Organization Of Agv and Phylogenetic Analysesmentioning

confidence: 77%

Identification and characterization of a novel geminivirus with a monopartite genome infecting apple trees

Liang

Navarro

Zhang

et al. 2015

Journal of General Virology

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A novel circular DNA virus sequence has been identified through next-generation sequencing and in silico assembly of small RNAs of 21-24 nt from an apple tree grown in China. The virus genome was cloned using two independent approaches and sequenced. With a size of 2932 nt, it showed the same genomic structure and conserved origin of replication reported for members of the family Geminiviridae. However, the low nucleotide and amino acid sequence identity with known geminiviruses indicated that it was a novel virus, for which the provisional name apple geminivirus (AGV) is proposed. Rolling circle amplification followed by RFLP analyses indicated that AGV was a virus with a monopartite DNA genome. This result was in line with bioassays showing that the cloned viral genome was infectious in several herbaceous plants (Nicotiana bethamiana, Nicotiana glutinosa and Solanum lycopersicum), thus confirming it was complete and biologically active, although no symptoms were observed in these experimental hosts. AGV genome structure and phylogenetic analyses did not support the inclusion of this novel species in any of the established genera in the family Geminiviridae. A survey of 165 apple trees grown in four Chinese provinces showed a prevalence of 7.2 % for AGV, confirming its presence in several cultivars and geographical areas in China, although no obvious relationship between virus infection and specific symptoms was found.

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Section: Genome Organization Of Agv and Phylogenetic Analysesmentioning

confidence: 77%

Identification and characterization of a novel geminivirus with a monopartite genome infecting apple trees

Liang

Navarro

Zhang

et al. 2015

Journal of General Virology

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“…Comparative nucleotide homology of CP and partial AV2 and AC3 genes sequences indicate that, each of these genes have different evolutionary origin in the sunn hemp begomovirus. The plausible reason for this difference could be the genetic recombination between begomo-viruses (Zhou et al, 1998). Nawaz-ul-Rehmana et al (2009) have also suggested the Southeast Asia as a possible center of origin for molecular diversity of geminiviruses.…”

Section: Resultsmentioning

confidence: 99%

Characterization of Sunn hemp begomovirus and its geographical origin based on in silico structural and functional analysis of recombinant coat protein

Ahmad¹,

Singh²,

Al‐Khedhairy³

et al. 2011

Afr. J. Biotechnol.

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Phylogenetic data suggested that, the AV2, CP and AC3 genes have closest genetic relationship with begomovirus isolates from India, China and Bangladesh, respectively. In silico recombination analysis elucidated a 297 nucleotides hot spot (346 to 643 nucleotides) within AV2 overlapping region of CP gene, amenable to genetic rearrangements, with lineage from tomato leaf curl virus Bangalore (ToLCuVB) and Indian cassava mosaic virus-Ind (ICMV) as major and minor parents, respectively. Thus, it is concluded that the recombinant CP genes related to begomoviruses are evolved from the Indian isolates, causing broad host specificity and molecular diversity among the related begomoviruses across the geographical limits of Southeast Asia.

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“…Several begomoviruses causing Cotton leaf curl disease have been reported from these areas [22,30,36], all of which are found to be associated with betasatellites. Artificial inoculation experiments have shown that the betasatellites are necessary for producing typical symptoms of Cotton leaf curl disease [3].…”

Section: Discussionmentioning

confidence: 99%

A Geminivirus-Satellite Complex is Associated with Leaf Deformity of Mentha (Mint) Plants in Punjab

et al. 2010

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A widespread leaf deformity disease of mentha (mint), accompanied by whiteflies, the vectors of begomoviruses, was observed in Punjab in the last few years. The presence of begomovirus was indicated by DNA dot-blot analysis using the conserved coat protein and replicationassociated protein genes of another begomovirus, Sri Lankan cassava mosaic virus (SLCMV). A DNA fragment (2.0 kb), representing a partial genomic DNA of a begomovirus, amplified from the symptomatic mentha leaves was used to design end-primers and further amplify an additional 0.9 kb fragment, representing the remaining portion of the resident viral DNA. The two sequences, assembled together (2.7 kb), showed that they represented the complete sequence of an isolate of Tomato leaf curl Karnataka virus (ToLCKV) DNA. Using universal betasatellite primers, a 1.4 kb fragment was amplified from the same sample. This cloned DNA fragment showed complete sequence identity with the previously reported Cotton leaf curl Multan betasatellite (CLCuMB). Majority of the symptomatic mentha leaf samples, collected from four districts of Punjab, showed cross-hybridization in DNA dot-blot using cloned SLCMV and CLCuMB DNA, indicating the presence of one or more begomoviruses related to SLCMV and the betasatellite, CLCuMB. The begomovirus and betasatellite could be mechanically transmitted to Nicotiana benthamiana. Whitefly transmission of the resident begomovirus could also be demonstrated on mentha. The evidence indicates the association of ToLCKV and CLCuMB, a hitherto new combination of a begomovirus and a betasatellite associated with a leaf deformity disease in mentha in Punjab.

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Four DNA-A variants among Pakistani isolates of cotton leaf curl virus and their affinities to DNA-A of geminivirus isolates from okra.

Cited by 159 publications

References 15 publications

Identification and characterization of a novel geminivirus with a monopartite genome infecting apple trees

Identification and characterization of a novel geminivirus with a monopartite genome infecting apple trees

Characterization of Sunn hemp begomovirus and its geographical origin based on in silico structural and functional analysis of recombinant coat protein

A Geminivirus-Satellite Complex is Associated with Leaf Deformity of Mentha (Mint) Plants in Punjab

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