Fourier transform infrared imaging and microscopy studies of Pinus radiata pulps regarding the simultaneous saccharification and fermentation process

Castillo, Rosario; Araya, Juan; Troncoso, Eduardo; Vinet, Silenne; Freer, Juanita

doi:10.1016/j.aca.2015.01.032

Cited by 18 publications

(13 citation statements)

References 49 publications

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“…The treatment operating conditions may generate substrates with varying characteristics that influence the production of ethanol by the simultaneous saccharification and fermentation (SSF) process: (1) content of lignin and hemicelluloses, (2) pore volume, (3) crystallinity of cellulose, (4) distribution of residual lignin in the treated fibers, and (5) remaining arrangements between lignin and carbohydrates 4, 9, 10, 11. Opposite tendencies have been found between lignin and ethanol yield.…”

Section: Introductionmentioning

confidence: 99%

Biorefining of Pinus pinaster Stump Wood for Ethanol Production and Lignin Recovery

et al. 2021

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Stump wood is a forest residue having significant valorization potential. The aims of this work were to exploit pine stumps as a carbohydrate and lignin feedstock, and to convert the carbohydrate fraction to bioethanol by simultaneous saccharification and fermentation (SSF). Pinus pinaster stump wood (PPSW) was delignified by the alkaline organosolv (AOS) process. Soda (SA) cooking was used as benchmark process. Higher pulp yields were obtained in AOS treatments, whilst SA pulp showed the highest cellulose content. In the SSF of PPSW pulps, high ethanol concentrations were obtained with high productivity and yield. AOS lignins were purer and had lower molar mass and dispersity index, which are important parameters in high‐value co‐product applications.

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Section: Introductionmentioning

confidence: 99%

Biorefining of Pinus pinaster Stump Wood for Ethanol Production and Lignin Recovery

et al. 2021

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“…Moreover, results of the present study are consistent with previous studies published by our research group. Thus, it this form of lignin is identified as an important factor for the high conversion of cellulose to glucose, the heterogeneous redistribution of lignin, resulting in, a significant decrease in the recalcitrance of pretreated materials 22,34,35 . In the pretreatment, when hemicellulose is removed, the lignin is more exposed to changes that promote its redistribution in the form of micro-droplets, generating random areas on the fibers with lower lignin content.…”

Section: Confocal Laser Microscopy (Clm)mentioning

confidence: 99%

Chemical and Microstructural Changes in Eucalyptus Globulus Fibers Subjected to Four Different Pretreatments and Their Influence on the Enzymatic Hydrolysis

Troncoso¹,

Castillo²,

Valenzuela³

et al. 2017

J. Chil. Chem. Soc.

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In order to understand the relation between chemical composition, microscopic structure and enzymatic digestibility, different Eucalyptus globulus wood pretreated samples were examined. Pretreated materials obtained by steam explosion and autohydrolysis were compared with those obtained by organosolv and kraft processes. Chemical analyses of pretreated materials showed a decrease in the content of xylans, except in the kraft pulp. FT-IR spectra showed that the residual lignin in autohydrolysis pulp had experienced greater changes compared to those in steam explosion and organosolv pulps, whereas minor changes in lignin kraft pulp were observed. The fiber morphology indicated that autohydrolysis pretreatment was the most aggressive treatment. Reduction in the content of lignin and its redistribution on the fiber wall were confirmed through confocal laser microscopy. The formation of discrete lignin droplets deposited on the surface of the fibers was observed in all pretreatments, with a higher frequency in organosolv followed by steam explosion. A significant increase in enzymatic accessibility was achieved in organosolv, autohydrolysis and steam explosion pulps, due to xylans removal combined with lignin redistribution. Homogeneous lignin distribution and higher xylan content may be related to the low enzymatic hydrolysis efficiency in kraft pulp.

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“…For example, the relative concentration and spectral intensity values cannot be negative; therefore, iterations between the MCR algorithms were carried out under non-negative constraints. The application of multivariate curve resolution with alternating least squares (MCR-ALS) for the analysis of FT-IR images is detailed in a previous publication [25].…”

Section: Chemometric Analysismentioning

confidence: 99%

“…However, it has also been proposed that the removal of hemicelluloses accompanied by the formation of lignin micro-droplets allows better enzyme action and catalytic adsorption to cellulose. As a result, there would be an increase in conversion rates during enzymatic hydrolysis because these droplets can migrate to certain regions of the cell wall, exposing the cellulose to the enzymes [19,25]. To demonstrate this proposal, in this study, Eucalyptus globulus woodchips were pre-treated by SE at different severity conditions.…”

Section: Introductionmentioning

confidence: 95%

Effects on Lignin Redistribution in Eucalyptus globulus Fibres Pre-Treated by Steam Explosion: A Microscale Study to Cellulose Accessibility

et al. 2021

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The objective of this study was to investigate structural changes and lignin redistribution in Eucalyptus globulus pre-treated by steam explosion under different degrees of severity (S0), in order to evaluate their effect on cellulose accessibility by enzymatic hydrolysis. Approximately 87.7% to 98.5% of original glucans were retained in the pre-treated material. Glucose yields after the enzymatic hydrolysis of pre-treated material improved from 19.4% to 85.1% when S0 was increased from 8.53 to 10.42. One of the main reasons for the increase in glucose yield was the redistribution of lignin as micro-particles were deposited on the surface and interior of the fibre cell wall. This information was confirmed by laser scanning confocal fluorescence and FT-IR imaging; these microscopic techniques show changes in the physical and chemical characteristics of pre-treated fibres. In addition, the results allowed the construction of an explanatory model for microscale understanding of the enzymatic accessibility mechanism in the pre-treated lignocellulose.

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Fourier transform infrared imaging and microscopy studies of Pinus radiata pulps regarding the simultaneous saccharification and fermentation process

Cited by 18 publications

References 49 publications

Biorefining of Pinus pinaster Stump Wood for Ethanol Production and Lignin Recovery

Biorefining of Pinus pinaster Stump Wood for Ethanol Production and Lignin Recovery

Chemical and Microstructural Changes in Eucalyptus Globulus Fibers Subjected to Four Different Pretreatments and Their Influence on the Enzymatic Hydrolysis

Effects on Lignin Redistribution in Eucalyptus globulus Fibres Pre-Treated by Steam Explosion: A Microscale Study to Cellulose Accessibility

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