Fourier transform infrared spectrometer analysis and antimicrobial screening of ethanolic extract of Operculina terpathum from cholistan desert

Abstract

Aim of present study was to assess pharmacological (antioxidant, antibacterial & antifungal) potential of Operculina terpathum seeds. Ethanolic extract was prepared and its phytochemical evaluation show the different chemical compounds such as carbohydrates, phenols, tannin, flavonoids, cardiac glycosides, steroids, alkaloids and proteins. FTIR spectra showed the presence of organic acids, hydroxyl and phenolic compounds, amino groups, aliphatic compounds, functional groups such as amide, ketone, aldehyde, aromatics and halogen compounds. Antioxidant activity of the Operculina terpathum alcoholic extract was performed by DPPH method and it showed 97.13%whereas IC50±SEM (µg/ml) was 1.425±0.16. Antibacterial activity was performed against different bacterial strains and results were comparable with that of standard. Maximum antibacterial activity was exhibited by Bacillus subtillis (28.33±2 mm) and Bacillus pumilus (25.33±2 mm) respectively. Antifungal activity was also performed and it showed maximum activity against Aspergillus flavous and Candida albicans6±1, 5±1mm respectively. These results showed that Operculina terpathum has good antibacterial and antifungal activity against different microbes and it could be used as an alternative to antibiotics, as the antibiotics resistance is very common now a days

Biological Synthesis and Characterization of Silver-Doped Nanocomposites: Antibacterial and Mechanistic Studies

“…Moreover, two distinct peaks, which were identified at 2912 cm −1 and 3287 cm −1 , are potentially linked to the symmetrical elongation vibrations of the CH 2 -CH 3 group of an alkane and the O-H bonds linked to alcohols and phenolic compounds, respectively. These chemical groups are well known to exist in plant extracts as evidenced by previously published research papers [43][44][45]. The FTIR spectrum (orange color) of AgNPs revealed major peaks at wavelengths of 3300 cm −1 (OH bond stretching) and 1634 cm −1 (carbonyl C=O group), which correspond to the involvement of hydroxyl and carboxylic acid moieties (encountered in polyphenols, phenolic acids and proteins) of C. macrostachyus extract in the reduction of the silver ions (Ag + ).…”

Biological Synthesis and Characterization of Silver-Doped Nanocomposites: Antibacterial and Mechanistic Studies

“…Moreover, two distinct peaks, which were identified at 2912 cm -1 and 3287 cm -1 are potentially linked to the symmetrical elongation vibrations of the CH2-CH3 group of an alkane and the O-H bonds linked to alcohols and phenolic compounds, respectively. These chemical groups are well known to exist in plant extracts as evidenced by previously published research papers [39][40][41]. The FTIR spectrum (orange color) of AgNPs revealed major peaks at wavelength 3300 cm -1 (OH bond stretching) and 1634 cm -1 (carbonyl C=O group), which corresponded to the involvement of hydroxyl and carboxylic acid moieties (encountered in polyphenols, phenolic acids and proteins) of C. macrostachyus extract in the reduction of the silver ions (Ag + ).…”

Evaluation of the Synergistic Effect of Thymol/Ampicillin on Escherichia coli strains

“…The regular deconvolution method was used to study the infrared spectroscopy of thymol and ampicillin separately and in combination (17,18). To do this, the samples were powdered and dispersed in KBr pellets, and then recorded using a Burker TENSOR 27 instrument.…”

“…To do this, the samples were powdered and dispersed in KBr pellets, and then recorded using a Burker TENSOR 27 instrument. The FTIR spectrometer (Thermo Scientific, USA) was used (17,18).…”