FOXA1 Leads to Aberrant Expression of SIX4 Affecting Cervical Cancer Cell Growth and Chemoresistance

Wang, Zhuo; Sun, Baosheng; Chen, Zhi-Shen; Zhao, Kangkang; Wang, Yun-Long; Meng, Fanxu; Zhang, Yang

doi:10.1155/2022/9675466

Cited by 6 publications

(5 citation statements)

References 32 publications

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“…As previously described, 16 the potential binding site was predicted using a bioinformatics tool (https://jaspar.genereg.net/), and amplification of the related region was performed using PCR. Subsequently, the amplified gene was inserted into the pGL3 vector (Promega, USA).…”

Section: Methodsmentioning

confidence: 99%

“…ChIP analysis was performed as previously described 16 . A commercial ChIP kit (Sigma–Aldrich, USA) was obtained and used according to the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

“…ChIP analysis was performed as previously described. 16 A commercial ChIP kit (Sigma-Aldrich, USA) was obtained and used according to the manufacturer's instructions. Cells were cross-linked with 1% formaldehyde solution at 25 C for 15 min and quenched by glycine.…”

Section: Chip Analysismentioning

confidence: 99%

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FOXA1 prolongs S phase and promotes cancer progression in non‐small cell lung cancer through upregulation of CDC5L and activation of the ERK1/2 and JAK2 pathways

Chen,

Wei,

et al. 2023

The Kaohsiung J of Med Scie

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Non‐small cell lung cancer (NSCLC) causes high mortality worldwide; however, its molecular pathways have not been fully investigated. The relationship between FOXA1 and CDC5L as well as their roles in NSCLC have not been comprehensively studied. Clinical tissues were collected from 78 NSCLC patients for clinical studies. The BEAS‐2B human normal lung epithelial cell line and the A549, Calu‐3, H526 and H2170 human NSCLC cell lines were used for in vitro studies. sh‐FOXA1 and oe‐CDC5L constructs were used to generate knockdown and overexpression models, respectively. The CCK‐8 assay was used to analyze cell viability. The cell cycle and apoptosis were evaluated by flow cytometry analysis. The relationship between FOXA1 and CDC5L was demonstrated using dual‐luciferase and ChIP assays. Gene levels were examined via immunohistochemistry, qRT‐PCR and western blot analysis. FOXA1 levels were increased in NSCLC clinical tissues and cell lines. Depletion of FOXA1 increased the apoptosis rate and increased the proportion of cells in G2/M phase. In addition, we demonstrated that FOXA1 was directly bound to the promoter of CDC5L and that depletion of FOXA1 inhibited CDC5L expression. Overexpression of CDC5L induced ERK1/2 phosphorylation, induced JAK2 phosphorylation, inhibited cell apoptosis, prolonged S phase, and significantly reversed the effects of FOXA1 knockdown on the progression of NSCLC. The present study demonstrated that FOXA1 prolongs S phase and promotes NSCLC progression through upregulation of CDC5L and activation of the ERK1/2 and JAK2 pathways.

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Section: Methodsmentioning

confidence: 99%

“…ChIP analysis was performed as previously described 16 . A commercial ChIP kit (Sigma–Aldrich, USA) was obtained and used according to the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

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FOXA1 prolongs S phase and promotes cancer progression in non‐small cell lung cancer through upregulation of CDC5L and activation of the ERK1/2 and JAK2 pathways

Chen,

Wei,

et al. 2023

The Kaohsiung J of Med Scie

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“…Tumor staging was done according to International Federation of Gynecology and Obstetrics (FIGO) staging system that correlates with the widely used TNM classification system (14) . Histological sub-type was done according to The World Health Organization (WHO) classification (2020) of EOC (12) . Immunohistochemichal staining:…”

Section: Histopathological Evaluationmentioning

confidence: 99%

“…Poor prognosis in hepatocellular carcinoma and the development of metastatic prostatic cancer are linked to high levels of FOX A1 expression (11) , and in cervical carcinoma is linked to treatment resistance (12) .…”

Section: Introductionmentioning

confidence: 99%

Value of Forkhead Box A1 (FOX A1) Immunohistochemical Expression in Epithelial Ovarian Carcinoma

Mohamed¹,

Mustafa²,

Harb³

et al. 2023

The Egyptian Journal of Hospital Medicine

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Background: Ovarian cancer is with the greatest fatality rate in the gynecological malignancy, with about two-thirds of patients receiving an advanced diagnosis because of late presentation. Additionally, 90% of patients experience recurrence and eventually develop chemo resistance. Finding new prognostic indicators and treatment targets tailored to that cancer is therefore highly desirable. It was discovered that FOX A1 plays a part in the growth and development of numerous tumours, including gliomas, breast, stomach, lung, and esophageal cancers, although its function in ovarian cancer has not been fully characterised. Aim: To assess the value of FOX A1 immunohistochemical expression in epithelial ovarian cancer and its relationship with clinicopathologic features. Materials and methods: This is a retrospective cohort study that was carried out in the Pathology department, Faculty of Medicine, Zagazig University, in the period from November 2021to November 2022. All cases of epithelial ovarian cancer of different; grades, stages and histopathological subtypes with complete clinical data were included in the study. Specimens of paraffin blocks were obtained by total abdominal hysterectomy with bilateral salpingoophorectomy (TAH+BSO) with omentectomy and lymph node dissection. They were examined by two independent pathologist for evaluation grade and stage. FOX A1 immunohistochemical staining was done for all specimens. Results: There was a statistically significant association between FOX A1 expression and patients' age, menopause, patients with bilateral lesions, ruptured capsule, positive peritoneal cytology, lymph node metastasis, omental deposits pathological grade and FIGO stage.-Conclusion: FOX A1 expression was related to poor prognostic predictors in EOC.

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Forkhead box proteins as the critical regulators of cisplatin response in tumor cells

Maharati,

Moghbeli

2023

European Journal of Pharmacology

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FOXA1 Leads to Aberrant Expression of SIX4 Affecting Cervical Cancer Cell Growth and Chemoresistance

Cited by 6 publications

References 32 publications

FOXA1 prolongs S phase and promotes cancer progression in non‐small cell lung cancer through upregulation of CDC5L and activation of the ERK1/2 and JAK2 pathways

FOXA1 prolongs S phase and promotes cancer progression in non‐small cell lung cancer through upregulation of CDC5L and activation of the ERK1/2 and JAK2 pathways

Value of Forkhead Box A1 (FOX A1) Immunohistochemical Expression in Epithelial Ovarian Carcinoma

Forkhead box proteins as the critical regulators of cisplatin response in tumor cells

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