FOXL2 down-regulates vitellogenin expression at mature stage in <i>Eriocheir sinensis</i>

Xie, Jing; Wang, Yuanli; Yang, Hongdan; Duan, Zelin; Wang, Qun

doi:10.1042/bsr20150151

Cited by 35 publications

(22 citation statements)

References 46 publications

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“…Interestingly, the expression level of foxl2 was increased slightly, rather than decreased, in the knockdown ovary and testis comparing to that of the control group. In most species, foxl2 is predominantly expressed in ovarian granulosa cells and is required for ovary differentiation and maintenance [36,37,38,39,40]. Recently, it has been reported that the foxl2 transcript is expressed in both the ovary and testis of M. nudus [19].…”

Section: Discussionmentioning

confidence: 99%

Molecular Cloning and Sexually Dimorphic Expression Analysis of nanos2 in the Sea Urchin, Mesocentrotus nudus

Zhang

Han

Wang

et al. 2019

IJMS

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Sea urchin (Mesocentrotus nudus) is an economically important mariculture species in China and the gonads are the solely edible parts to human. The molecular mechanisms of gonad development have attracted increasing attention in recent years. Although the nanos2 gene has been identified as a germ cell marker in several invertebrates, little is known about nanos2 in adult sea urchins. Hereinto, we report the characterization of Mnnano2, an M. nudus nanos2 homology gene. Mnnanos2 is a maternal factor and can be detected continuously during embryogenesis and early ontogeny. Real-time quantitative PCR (RT-qPCR) and section in situ hybridization (ISH) analysis revealed a dynamic and sexually dimorphic expression pattern of Mnnano2 in the gonads. Its expression reached the maximal level at Stage 2 along with the gonad development in both ovary and testis. In the ovary, Mnnanos2 is specifically expressed in germ cells. In contrast, Mnnanos2 is expressed in both nutritive phagocytes (NP) cells and male germ cells in testis. Moreover, knocking down of Mnnanos2 by means of RNA interference (RNAi) reduced nanos2 and boule expression but conversely increased the expression of foxl2. Therefore, our data suggest that Mnnanos2 may serve as a female germ cell marker during gametogenesis and provide chances to uncover its function in adult sea urchin.

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Section: Discussionmentioning

confidence: 99%

Molecular Cloning and Sexually Dimorphic Expression Analysis of nanos2 in the Sea Urchin, Mesocentrotus nudus

Zhang

Han

Wang

et al. 2019

IJMS

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“…FOXL2 negatively regulates the VTG synthesis at developmental stage. FOXL2 downregulates VTG's expression by binding DDX20 in the regulation of follicular cell apoptosis and repress the synthesis of VTG when bound with FTZ-F1 at the mature stage [ 18 , 34 , 35 ]. According to our research, during the process of testis development, FOXL2 had no obvious change, and FTZ-F1 gene was downregulated.…”

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptome Analysis Reveals Related Regulatory Mechanisms of Androgenic Gland inEriocheir sinensis

Zeng

et al. 2017

BioMed Research International

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Chinese mitten crab (Eriocheir sinensis) is one of the most commercially important aquaculture species in China. The androgenic gland (AG) of crustaceans plays pivotal roles in the regulation of male differentiation and in maintaining the male sexual characteristics. In order to reveal related mechanisms in AG, we compared transcriptomes of AG between proliferation and secretion phase. A total of 72,000 unigenes and 4,027 differentially expressed genes were obtained. Gene ontology enrichment analysis indicated that biological processes and metabolic pathways related to protein synthesis and secretion such as transcription, translation, and signal transduction were significantly enriched. Critical genes such as IAG, SXL, TRA-2, SRY, FTZ-F1, FOXL2, and FEM-1 were identified and potentially involved in maintaining the testis development and spermatogenesis. Ribosomes pathway revealed the cause of insulin-like androgenic gland hormone secretion increase. Three insulin-like receptors were thought to be associated with growth and spermatogenesis. In the neuroactive ligand-receptor interaction pathway, the expression of octopamine receptor, 5-HT receptor 1, and melatonin receptor was significantly changed, which revealed the key regulation mechanism of aggressive and mating behavior of males. Comparative transcriptome analysis provided new insights into the genome-wide molecular mechanisms of AG development and the regulatory mechanisms of male development.

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“…1 , table 1 ), including sponges (Suberites domuncula) [Adell and Müller, 2004], mollusks [Naimi et al, 2009;Teaniniuraitemoana et al, 2014;Zhang et al, 2014;Tong et al, 2015], arthropods including insects and crustaceans [De Loof et al, 2010;Ma et al, 2012;Farlora et al, 2014;Li et al, 2015], hemichordates [Fritzenwanker et al, . See text for details.…”

Section: The Complexity Of Foxl2 Gene Evolutionmentioning

confidence: 99%

Foxl2 and Its Relatives Are Evolutionary Conserved Players in Gonadal Sex Differentiation

Bertho

Pasquier

Pan

et al. 2016

Sex Dev

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Foxl2 is a member of the large family of Forkhead Box (Fox) domain transcription factors. It emerged during the last 15 years as a key player in ovarian differentiation and oogenesis in vertebrates and especially mammals. This review focuses on Foxl2 genes in light of recent findings on their evolution, expression, and implication in sex differentiation in animals in general. Homologs of Foxl2 and its paralog Foxl3 are found in all metazoans, but their gene evolution is complex, with multiple gains and losses following successive whole genome duplication events in vertebrates. This review aims to decipher the evolutionary forces that drove Foxl2/3 gene specialization through sub- and neo-functionalization during evolution. Expression data in metazoans suggests that Foxl2/3 progressively acquired a role in both somatic and germ cell gonad differentiation and that a certain degree of sub-functionalization occurred after its duplication in vertebrates. This generated a scenario where Foxl2 is predominantly expressed in ovarian somatic cells and Foxl3 in male germ cells. To support this hypothesis, we provide original results showing that in the pea aphid (insects) foxl2/3 is predominantly expressed in sexual females and showing that in bovine ovaries FOXL2 is specifically expressed in granulosa cells. Overall, current results suggest that Foxl2 and Foxl3 are evolutionarily conserved players involved in somatic and germinal differentiation of gonadal sex.

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FOXL2 down-regulates vitellogenin expression at mature stage in Eriocheir sinensis

Cited by 35 publications

References 46 publications

Molecular Cloning and Sexually Dimorphic Expression Analysis of nanos2 in the Sea Urchin, Mesocentrotus nudus

Molecular Cloning and Sexually Dimorphic Expression Analysis of nanos2 in the Sea Urchin, Mesocentrotus nudus

Comparative Transcriptome Analysis Reveals Related Regulatory Mechanisms of Androgenic Gland inEriocheir sinensis

Foxl2 and Its Relatives Are Evolutionary Conserved Players in Gonadal Sex Differentiation

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