Highlights A key problem in neural spike sorting is highlighted. A new method to measure the position of action potentials in real-time is described. This method is validated using detailed simulations. Major improvements are demonstrated compared to standard methods.
AbstractBackground: This paper describes a new method to calculate the positions of action potentials (APs) in extracellular neural recordings in real-time. Extracellular recordings made using various types of electrode structures are a key tool in experimental electrophysiology and involve the identification of signals from individual neurons, a process known by the generic name spike sorting. An essential feature of the process is the alignment of individual APs ('spikes') in time. Given the recordings are invariably made in noisy environments there is a tendency to timing jitter leading to alignment errors. This is especially true if single point methods of AP location are employed. New Method: The problems of single point AP location in the presence of noise are significantly reduced by the use of the centroid as a measure of position, which is based on a time average of all the points in the AP. The method is described and analysed in detail and it is shown that a very economical hardware realisation is possible.
Results:The new methods are investigated by simulation using deterministic models of nerve signals (i.e. APs with added noise; both correlated and uncorrelated noise models are considered) as well as results measured from a hardware implementation. A power efficient realisation of the centroid filter is demonstrated using a complex programmable logic device (CPLD).
Comparison with Existing Method:Detailed simulations demonstrate the superiority of the centroid method compared to several standard, single point metrics. Conclusions: A new method has been suggested that significantly improves one of the key issues in neural spike sorting. The technique has the potential to influence significantly the design of electrophysiological recording systems in the future.