Fractionation of nuclei from induced hepatomas by sucrose gradient centrifugation

Albrecht, Christin

doi:10.1016/0014-4827(69)90391-7

Cited by 8 publications

(4 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Differences in sedimentation velocity allowed the separation of diploid, tetraploid, and octoploid nuclei from rat liver (1,6), implying that these nuclei differed in size or in density.…”

Section: Discussionmentioning

confidence: 99%

Studies on Nuclei of Paramecium aurelia. I. Isolation and Purification by Continuous or Discontinuous Sucrose Gradient Centrifugation and Biochemical Composition.*

Isaacks

Santos

Musil

1973

The Journal of Protozoology

View full text Add to dashboard Cite

SYNOPSIS. Nuclei of Paramecium aurelia were isolated and purified by a new method involving the use of continuous or discontinuous sucrose gradient centrifugation. As judged from the DNA levels or nuclear counts in the purified samples, 21–22% of the nuclei were recovered by this method. The single density of the nuclei estimated on the basis of linear sucrose gradients was between 1.35 ± 0.01 and 1.36 ± 0.01. The average picogram quantities of total protein, DNA, and RNA per nucleus were 435, 62.2, and 51.2, respectively.

show abstract

“…Differences in sedimentation velocity allowed the separation of diploid, tetraploid, and octoploid nuclei from rat liver (1,6), implying that these nuclei differed in size or in density.…”

Section: Discussionmentioning

confidence: 99%

Studies on Nuclei of Paramecium aurelia. I. Isolation and Purification by Continuous or Discontinuous Sucrose Gradient Centrifugation and Biochemical Composition.*

Isaacks

Santos

Musil

1973

The Journal of Protozoology

View full text Add to dashboard Cite

show abstract

“…In experiment III, hepatic nuclei were separated into two major size classes on the basis of differences in their velocity of sedimentation. This technique had been employed previously to effect separation ( at unit gravity) in 30 to 36 hours (Falzone, Barrows, and Yiengst 1952); however, the potential of the method was further exploited by the use of low speed centrifugation to achieve more rapid separations in conventional sucrose gradients (Albrecht 1958(Albrecht , 1969 and in zonal rotors (Johnston et al 1968 a,b). Nuclei also have been separated into density classes by isopycnic centri fugation (Fisher, Holbrook, and Irvin 1963)=…”

Section: A Suppression Of Polyploidizationmentioning

confidence: 99%

Ploidy and biochemical characteristics of liver cell nuclei in rats fed different amounts of dietary protein

Moore

View full text Add to dashboard Cite

of analysis of variance for data 165 on hepatic DNA concentration (mg/g liver) in experiment III. XIII. Summary of analysis of variance for data 165 on hepatic RNA concentration (mg/g liver) in experiment II. XXV. Summary of analysis of variance for data 167 on hepatic RNA concentration (mg/g liver) in experiment III. XV. Summary of analysis of variance for data 158 on hepatic protein concentration (mg/g liver) in experiment II.

show abstract

“…This technique had been employed previously to effect separation ( at unit gravity) in 30 to 36 hours (Falzone, Barrows, and Yiengst 1952); however, the potential of the method was further exploited by the use of low speed centrifugation to achieve more rapid separations in conventional sucrose gradients (Albrecht 1958(Albrecht , 1969 and in zonal rotors (Johnston et al 1968 a,b). Nuclei also have been separated into density classes by isopycnic centri fugation (Fisher, Holbrook, and Irvin 1963)= Although the nuclear classes obtained in the present studies were discrete on the basis of sedimentation prop erties, three other criteria were examined to confirm that the nuclei thus isolated were diploid and tetraploid.…”

Section: A Suppression Of Polyploidizationmentioning

confidence: 99%

“…In addition, the method has been used to study distribution of diploid and tetraploid nuclei in hepatomas (Albrecht 1969). …”

Section: Density Gradient Separation Of Diploid and Tetraploid Nucleimentioning

confidence: 99%

Ploidy and biochemical characteristics of liver cell nuclei in rats fed different amounts of dietary protein

Moore

View full text Add to dashboard Cite

Fractionation of nuclei from induced hepatomas by sucrose gradient centrifugation

Cited by 8 publications

References 9 publications

Studies on Nuclei of Paramecium aurelia. I. Isolation and Purification by Continuous or Discontinuous Sucrose Gradient Centrifugation and Biochemical Composition.*

Studies on Nuclei of Paramecium aurelia. I. Isolation and Purification by Continuous or Discontinuous Sucrose Gradient Centrifugation and Biochemical Composition.*

Ploidy and biochemical characteristics of liver cell nuclei in rats fed different amounts of dietary protein

Ploidy and biochemical characteristics of liver cell nuclei in rats fed different amounts of dietary protein

Contact Info

Product

Resources

About