Frameshift mutagenesis in bacteria by 8-methoxypsoralen (methoxalen) in the dark

Cell killing and mutagenesis by 8-methoxypsoralen in mammalian (rodent) cells

“…The negative effect of 8-MOP in the dark on this system might be a consequence of either a specific failure to mutate the genes involved at this locus or a weak potential for mutation. However the combination of positive mutation in the HGPRT system and the absence of mutation with the Oua system is consistent with the molecular mechanism of dark 8-MOP mutagenesis inferred from studies with bacteria which suggest that it is a frameshift mutagen (Clarke & Wade, 1975;Bridges & Mottershead, 1977). The HGPRT system, being concerned with a non-vital function, is susceptible to a large array of mutational events including deletions or frameshift mutations which appear operationally as deletions.…”

“…The 8-MOP used throughout this study was obtained from Upjohn and is the same batch as that used by Bridges & Mottershead (1977). It was dissolved in Aristar grade ethanol (British Drug Houses) and pre-warmed to 37 C before direct addition to the medium immediately after removing cultures from the incubator.…”

Possible long-term hazards of photochemotherapy with psoralens and near ultraviolet light

“…This interaction may have biological consequences. It results in protection against UVB damage in bacteria (Bridges, 1971) and apparently, in mice (Griffin, 1959) it may also inhibit DNA repair in bacteria and is mutagenic both in bacteria (where it causes frameshift mutations (Clarke & Wade, 1975;Bridges & Mottershead, 1977)) and in cultured Chinese hamster cells (Arlett & Broughton, personal communication). An estimate of genetic effect due to psoralen in the gonads, suggests, however, that the risk to man is relatively small.…”

Photobiological Properties of a Novel, Naturally Occurring Furoisocoumarin, Coriandrin

“…Reversion to prototrophy can occur by true reversions at the tryptophan locus or by induction of ochre suppressors (Igali et al, 1970). Frame shift mutagenesis was measured in E. coli lac-, thiamineas described by Bridges and Mottershead (1977) and Ashwood-Smith (1978).…”